LC-HRMS Profiling and Antidiabetic, Anticholinergic, and Antioxidant Activities of Aerial Parts of Kınkor (Ferulago stellata)

Abstract

Kınkor (Ferulago stellata) is Turkish medicinal plant species and used in folk medicine against some diseases. As far as we know, the data are not available on the biological activities and chemical composition of this medicinal plant. In this study, the phytochemical composition; some metabolic enzyme inhibition; and antidiabetic, anticholinergic, and antioxidant activities of this plant were assessed. In order to evaluate the antioxidant activity of evaporated ethanolic extract (EEFS) and lyophilized water extract (WEFS) of kınkor (Ferulago stellata), some putative antioxidant methods such as DPPH· scavenging activity, ABTS•+ scavenging activity, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+)-binding activities were separately performed. Furthermore, ascorbic acid, BHT, and α-tocopherol were used as the standard compounds. Additionally, the main phenolic compounds that are responsible for antioxidant abilities of ethanol and water extracts of kınkor (Ferulago stellata) were determined by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). Ethanol and water extracts of kınkor (Ferulago stellata) demonstrated effective antioxidant abilities when compared to standards. Moreover, ethanol extract of kınkor (Ferulago stellata) demonstrated IC50 values of 1.772 μg/mL against acetylcholinesterase (AChE), 33.56 ± 2.96 μg/mL against α-glycosidase, and 0.639 μg/mL against α-amylase enzyme respectively.

Polyphenol Contents, Potential Antioxidant, Anticholinergic and Antidiabetic Properties of Mountain Mint (Cyclotrichium leucotrichum)

“…Recently, it was shown that 6.7 and 4.9 mg of QE flavonoids were found in g lyophilized water and evaporated ethanolic extracts of Ferula orientalis. [2] Also, 27.30 and 11.75 mg QE of flavonoids was found for ethanolic and water extracts Convulvulus betonicifolia, respectively. [3] Similarly, it was shown that 1.480 and 0.328 mg of QE flavonoids per 1 g dried extracts were found in ethanol and water extracts of avocado.…”

“…However, Turkey has a wide plant biodiversity for use in these areas. [2,3] Also, biologically active secondary metabolites are a large group of chemicals with small molecular weights and widely used in folk medicine for their well-known potential for biological, chemical and physiological abilities including anti-inflammatory, antidiabetic, neuroprotective and antioxidant features. [4,5] The Lamiaceae family is a large plant family with hundreds of biologically active secondary plant metabolites.…”

Benzimidazolium salts bearing the trifluoromethyl group as organofluorine compounds: Synthesis, characterization, crystal structure, in silico study, and inhibitory profiles against acetylcholinesterase and α‐glycosidase

“…This enzyme hydrolyzes carbohydrates by acting on 1,4-α bonds and releases D-glucose from the nonreducing end of the sugar. [37][38][39] It plays a key role in carbohydrate metabolism in plants, animals, and microorganisms. It is quite common in microorganisms and has been characterized by purifying its extracellular, intracellular, or cell-bound forms from various bacteria, yeasts, and molds.…”

Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol

“…Antioxidants have been shown to play a crucial role in delaying or avoiding T2DM and AD [ 9 ]. One of the main goals in the treatment of T2DM is to provide inhibition studies of α-glycosidase, which is one of the most important digestive enzymes and catalyzes the breakdown of dietary polysaccharides [ 10 ].…”