LC-ESI-MS analysis, antioxidant, anti-diabetic and molecular docking studies on Corchorus depressus (L.) C.Chr

Abstract

The present study encompasses the ethnomedicinal consumption of Corchorus depressus (L.) C.Chr. (C. depressus) for diabetes. Plant samples were subjected to LC-ESI-MS analyses. The n-hexane, methanolic and water extracts were screened for -glucosidase inhibition and in vivo anti-diabetic studies. Further, antioxidant (DPPH) and antiinflammatory study was performed via luminol-enhanced chemiluminescence assay. The identified compounds were docked against the target enzymes of diabetes. The n-hexane fraction (CD-J1) showed IC 50 of 8.4 ± 0.1 µg mL -1 against-glucosidase enzyme. The subfractions CD-12 and CD-13 of CD-J1 obtained after flash column chromatography displayed further reduced IC 50 values of 4.3  0.1 and 6.3  0.1, respectively, as compared with standard drug acarbose (IC 50 values of 37.5 ± 0.2 µg mL -1 ). Simultaneously, dereplication of the most active sub-fraction CD-12 by LC-ESI-MS led to the identification of strophanthidin and some other active metabolites responsible for anti-diabetic activity. Molecular docking of strophanthidin with -glucosidase and -amylase revealed high affinity for these target enzymes.

Chemical and Nutritional Profiling of the Seaweed Dictyota dichotoma and Evaluation of Its Antioxidant, Antimicrobial and Hypoglycemic Potentials

“…The usage of herbal medicines for medical treatment has grown, and it is now crucial to screen medicinal plants for bioactive components [ 18 ]. Secondary metabolites are abundant in herbal products [ 19 ]. GC-MS is typically used to gather information and examine medicinal plants to identify their bioactive components.…”