LC-APCI+-MS/MS method for the analysis of ten hormones and two endocannabinoids in plasma and hair from the mice with different gut microbiota

Chu, Liuxi; Li, Na; Deng, Jia; Wu, Yan; Yang, H.; Wang, Wei; Zhou, Dongrui; Deng, Huihua

doi:10.1016/j.jpba.2020.113223

Cited by 20 publications

(13 citation statements)

References 40 publications

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“…In the murine species, steroids assessment in hair was previously reported in a very small number of studies and using different methodologies [28][29][30][31]51]. Overall, the results of the quantifications reported in this study are in agreement with existing literature, with the only exception of CORT that shows higher values when compared to some of the previous studies.…”

Section: Discussionsupporting

confidence: 90%

Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice

Elmi

Galligioni

Govoni

et al. 2020

Animals

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Steroids, providing information regarding several biological patterns including stress and sexual behavior, have been investigated in different matrices in laboratory mice. Data regarding hair quantification, indicative of longer timespans when compared to blood and saliva, are lacking. The aim of the work was to analyze the hormonal hair profile of laboratory male mice and to investigate potential relationships with age and housing, as a potential tool for welfare assessment. Fifty-six adult male C57BL/6J and C57BL/6OlaHsd substrain mice were included in the study, housed in pairs or groups. Testosterone (T) and dehydroepiandrosterone (DHEA) were quantified by radioimmunoassay, corticosterone (CORT) by ELISA. Mean hormone levels were 6.42 pg/mg for T, 23.16 pg/mg for DHEA and 502.1 pg/mg for CORT. Age influenced all hormones by significantly increasing T and DHEA levels and decreasing CORT; only DHEA, significantly higher in grouped mice, was influenced by housing conditions. The influence of age indicates the need for accurate age-related reference intervals, while the higher levels of DHEA in grouped animals suggests that such housing practice may be beneficial for social interactions. In conclusion, it seems that hair hormones quantification may be a good tool for welfare assessment in laboratory mice and may help in refining husbandry.

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Section: Discussionsupporting

confidence: 90%

Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice

Elmi

Galligioni

Govoni

et al. 2020

Animals

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“…Liquid chromatography (Agilent 1200 HPLC system; Agilent, Waldbronn, Germany) and tandem mass spectrometry (ABI 3200 Qtrap; ABI, Foster City, CA, USA) (liquid chromatography–mass spectrometry/mass spectrometry) was used to detect the concentrations of cortisol, cortisone, DHEA, testosterone, and progesterone in plasma. [ 5 6 ] The method showed good linearity ( R 2 > 0.99) in the range of 0.03–500 ng/mL, and the limit-of-quantitation were 0.1, 0.4, 0.6, 0.1, and 0.1 ng/mL for cortisol, cortisone, DHEA, testosterone, and progesterone, respectively. The coefficients of variation for intra-day and inter-day (n = 5) assays were less than 15%.…”

mentioning

confidence: 98%

Abnormal levels of cortisol and cortisone in patients with prurigo nodularis

Chu

Yang

et al. 2021

Indian J Dermatol

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“…blood via the portal system, affecting host systemic homeostasis. 94,95 In healthy humans, the gut mainly consists of intestinal mucosal epithelium, intestinal mucus, and intestinal flora and serves a mucosal barrier function. The normal intestinal barrier could effectively limit the access of bacterial compounds.…”

Section: Changes In the Gut Microbiota Associated With Liver Diseasementioning

confidence: 99%

“…During this process, in addition to liver and intestinal metabolites, endotoxins enter the liver with the blood via the portal system, affecting host systemic homeostasis. 94 95 …”

Section: Connection Between Gut Microbiota and Liver Diseasesmentioning

confidence: 99%

Clinical Application and Progress of Fecal Microbiota Transplantation in Liver Diseases: A Review

Qin

Zhang

et al. 2021

Semin Liver Dis

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The human gut harbors a dense and highly diverse microbiota of approximately 1,000 bacterial species. The interaction between the host and gut bacteria strongly influences human health. Numerous evidence suggest that intestinal flora imbalance is closely associated with the development and treatment of liver diseases, including acute liver injury and chronic liver diseases (cirrhosis, autoimmune liver disease, and fatty liver). Therefore, regulating the gut microbiota is expected to be a new method for the adjuvant treatment of liver diseases. Fecal microbiota transplantation (FMT) is defined as the transplantation of gut microbiota from healthy donors to sick patients via the upper or lower gastrointestinal route to restore the normal intestinal balance. In this study, we briefly review the current research on the gut microbiota and its link to liver diseases and then summarize the evidence to elucidate the clinical application and development of FMT in liver disease treatment.

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LC-APCI+-MS/MS method for the analysis of ten hormones and two endocannabinoids in plasma and hair from the mice with different gut microbiota

Cited by 20 publications

References 40 publications

Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice

Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice

Abnormal levels of cortisol and cortisone in patients with prurigo nodularis

Clinical Application and Progress of Fecal Microbiota Transplantation in Liver Diseases: A Review

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