Laying Performance, Fat Digestibility and Liver Condition of Laying Hens Supplemented With Vitamin B12 or Biotin and/ or Bile Acids in Diet

Abstract

For 8 weeks feeding trial, two hundred and seventy, 53 weeks old laying hens were used to investigate the effects of dietary supplementation of vitamin B12 or biotin and/or bile acids on performance, egg quality, fat digestibility and liver composition and histopathology. Birds were randomly divided into 6 groups (three replicates) and fed the experimental diets; group1 (G1) fed on the basal diet without additives as control while G2 and G3 supplemented with 0.02 ppm vitamin B12 and 0.3 mg biotin/kg diet respectively, groups 4-6 fed as the previous detailed design of G1-G3 with the addition of 400 g of dried bile acid (DBA) /tone feed. Biotin supplementation significantly (P˂0.05) increased body weight losses of laying hens, both vitamins significantly (P˂0.05) decreased daily feed intake (FI) and improved FCR. DBA addition alone or with biotin reduced these weight losses along with significant (P˂0.05) increase in daily FI. Vitamin B12 supplementation alone or with DBA increased average egg production (P˂0.05) while was reduced with biotin supplementation. Fat digestibility was non-significantly improved (P≥0.05) with both vitamins supplementation without or with DBA. Biotin significantly (P˂0.05) reduced the average yolk relative weight, which was increased when mixed with DBA, while significantly increased average egg albumin relative weight. Vitamin B12 or biotin addition without or with DBA non-significantly increased blood serum GOT and GPT activities, non-significantly reduced (P≥0.05) fat content of liver tissue (on dry matter or fresh basis) and serum lipid profile parameters except serum HDL concentration, was increased, with no histopathological changes in hepatic tissue. It could be summarized that vitamin B12 supplementation without or with DBA is recommended in layer diet as it gave the best performance, reduced serum lipid profile and improved fat digestibility and the hepatic health.

Effects of rumen-protected biotin on the growth performance, nitrogen utilization and blood parameters of yearling Liaoning cashmere doelings

“…For the physiological function of biotin is mainly related to the intermediary metabolism, including a series of carboxylation processes, et., PC, PCC, and M-CoA carboxylase [19]. This is consistent with biotin supplementa-tion research in non-ruminant species, that biotin significantly increased BW losses in laying hens [20]. The weight gain of mink kits also was better in fed a balanced diet with 0.1 mg/kg DM biotin than that of animals fed a low-biotin diet without biotin [7].…”

Effects of Processed Tomato (Lycopersicon esculentum) Wastes in the Diet on the Serum Lipid Profile of Laying Hens

“…The HDL level in this study was lower than the report from Mahata et al (2016), namely the HDL content in laying hens fed with boiled tomato powder to a level of 12% ranging from 37.75-48.67 Mg/dL. Blood HDL levels of laying hens supplemented with vitamin B12 and bile salts in the ration were 47.17-51.63 Mg/dL (El-Katcha et al, 2019). Basmacioglu and Ergul (2005) also stated that normal HDL levels in laying hens were >22 Mg/dL.…”

Dietary Fiber and Lysolecithin Supplementation in Growing Ducks: Effect on Performance, Immune Response, Intestinal Morphology and Lipid Metabolism-Regulating Genes

“…Serum lipid profile parameters including TG, LDL, and HDL are considered as the main indicators of lipid metabolism [40]. In the present study, DF% with or without lysolecithin non-significantly reduced TG and LDL and increased HDL serum concentrations.…”