Layer II of placental syncytiotrophoblasts expresses MDR1 and BCRP at the apical membrane in rodents

Akashi, Tomoya; Nishimura, Tomohiro; Takaki, Yoshiya; Takahashi, Masaya; Shin, Bo‐Chul; Tomi, Masatoshi; Nakashima, Emi

doi:10.1016/j.reprotox.2016.09.002

Cited by 12 publications

(9 citation statements)

References 30 publications

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“…It is now generally recognized that P-gp and BCRP in the placenta across species play important protective roles for the fetus from exposure to drugs and xenobiotics. Although the placental structure varies from species to species, P-gp and BCRP are all primarily localized on the apical membrane of syncytiotrophoblasts facing the maternal blood (Figures 1–3) [16,22,35–37]. The expression levels of P-gp and BCRP in the placenta change with gestational age in all species; however, the patterns of changes differ from species to species [22,31,35,58].…”

Section: Resultsmentioning

confidence: 99%

“…Earlier studies showed that P-gp was predominantly expressed on the apical membrane of the syncytiotrophoblast I layer in rat and mouse placentas [33,34]. However, recent studies [36,37] found that P-gp was mainly localized on the apical membrane of the syncytiotrophoblast II layer (Figure 2), but not the syncytiotrophoblast I layer. These studies suggest that both the syncytiotrophoblast I and II layers could play supportive and defensive roles.…”

Section: P-gp and Bcrp In The Placentamentioning

confidence: 93%

“…In mouse [68,69] or rat [68,70] placenta, Bcrp is also mainly expressed in the syncytiotrophoblast layers in the labyrinth and some vascular structures within the myometrium. Like P-gp, immunostaining analyses showed that Bcrp was on the apical membrane of the placental syncytiotrophoblast II layer, but not the syncytiotrophoblast I layer in rodents [36,37].…”

Section: P-gp and Bcrp In The Placentamentioning

confidence: 99%

“…Therefore, the direction of transport of Bcrp in fetal capillary endothelium is unknown. [32–37, 68–70]…”

Section: Figurementioning

confidence: 99%

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An update on expression and function of P-gp/ABCB1 and BCRP/ABCG2 in the placenta and fetus

Han

Gao

Mao

2018

Expert Opinion on Drug Metabolism & Toxicology

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P-glycoprotein (P-gp)/ABCB1 and breast cancer resistance protein (BCRP)/ABCG2 are highly expressed in the placenta and fetus throughout gestation and can modulate exposure and toxicity of drugs and xenobiotics to the vulnerable fetus during the sensitive times of growth and development. We aim to provide an update on current knowledge on placental and fetal expressions of the two transporters in different species, and to provide insight on interpreting transporter expression and fetal exposure relative to the concept of fraction of drug transported. Areas covered: Comprehensive literature review through PubMed (primarily from July 2010 to February 2018) on P-gp and BCRP expression and function in the placenta and fetus of primarily human, mouse, rat, and guinea pig. Expert opinion: While there are many commonalities in the expression and function of P-gp and BCRP in the placenta and fetal tissues across species, there are distinct differences in expression levels and temporal changes. Further studies are needed to quantify protein abundance of these transporters and functionally assess their activities at various gestational stages. Combining the knowledge of interspecies differences and the concept of fraction of drug transported, we may better predict the magnitude of impact these transporters have on fetal drug exposure.

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Section: Resultsmentioning

confidence: 99%

Section: P-gp and Bcrp In The Placentamentioning

confidence: 93%

Section: P-gp and Bcrp In The Placentamentioning

confidence: 99%

“…Therefore, the direction of transport of Bcrp in fetal capillary endothelium is unknown. [32–37, 68–70]…”

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

An update on expression and function of P-gp/ABCB1 and BCRP/ABCG2 in the placenta and fetus

Han

Gao

Mao

2018

Expert Opinion on Drug Metabolism & Toxicology

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“…Studies have indicated that syncytin-1 and estrogen stimulation induction is closely related, and which is in the cytotrophoblast fusion into the process of syncytium trophoblast key action [15]. It has been con rmed that the expression level of syncytin-1 is related to clinical manifestations in organ cancer patients [16], acute myeloid leukemia [17], breast cancer [18], endometrial carcinomas [19], ovarian cancer and colorectal cancer [20,21]. In this study, we con rmed the role of syncytin-1 in the carcinogenesis of PANC-1 and BxPC-3 cell lines, sow in 96 well plates for 24 h. Then, at different time points, each well is added with analytical reagent (beyotime, China), and then incubated.…”

Section: Introductionmentioning

confidence: 99%

Decreased miR- 31 Suppress Cell Migration and Proliferation By Targeting Syncytin-1 in Pancreatic Carcinoma

Yang¹,

Luo

Wang

et al. 2020

Preprint

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Background: Pancreatic cancer(PC) is seriously harmful to human health, and the pathogenesis is not clear. The present study aimed to explore the functional role of syncytin-1 in PC.Methods: Syncytin-1 and miR-31 expression was analyzed by qRT-PCR and Western blot analysis in both human PC cell lines and tissuse. The prognostic significance of syncytin-1 was investigated using the immunohistochemistry(IHC) and Kaplan-Meier survival. The CCK-8 assay and transwell assays were used to determine the role of syncytin-1 and miR-31 in cell proliferation, migration and invasion. Luciferase reporter assays was used to identify possible miRNA targets in tumorigenesis.Results: The results showed that the syncytin-1 level was significantly decreased in PC cell lines and tissues than normal(P < 0.05), while miR-31 was markedly higher than normal(P < 0.05), and low expression of syncytin-1 have a poor prognosis than high expression(P < 0.05). Overexpression of syncytin-1 significantly reduced the PC cell proliferation and invasion ability in PANC-1 and BxPC-3 cells(P < 0.05), and miR-31showed contrary results. The Dual-Luciferase reporter gene assay demonstrated that miR-31 binded directly to 3’UTR of syncytin-1 and resulting in the inhibition of syncytin-1. The overexpression of miR-31 promoted migration and proliferation of PC cells through down-regulating the expression of syncytin-1.Conclusion: We verified that syncytin-1 can inhibit proliferation and invasion of PC cell lines by targeting miR-31.

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Contributions of Drug Transporters to Blood-Placental Barrier

Liu

2019

Advances in Experimental Medicine and Biology

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Layer II of placental syncytiotrophoblasts expresses MDR1 and BCRP at the apical membrane in rodents

Cited by 12 publications

References 30 publications

An update on expression and function of P-gp/ABCB1 and BCRP/ABCG2 in the placenta and fetus

An update on expression and function of P-gp/ABCB1 and BCRP/ABCG2 in the placenta and fetus

Decreased miR- 31 Suppress Cell Migration and Proliferation By Targeting Syncytin-1 in Pancreatic Carcinoma

Contributions of Drug Transporters to Blood-Placental Barrier

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