Layer‐by‐Layer Buildup of Poly(<scp>L</scp>‐glutamic acid)/Chitosan Film for Biologically Active Coating

Song, Zhijiang; Yin, Jingbo; Luo, Kun; Zheng, Yan‐Zhen; Yang, Yan; Li, Qiong; Yan, Shifeng; Chen, Xuesi

doi:10.1002/mabi.200800164

Cited by 71 publications

(61 citation statements)

References 55 publications

(75 reference statements)

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“…In contrast, (chitosan/PGA-TriEG-Diclof) n films were considerably thinner with no observed induction period, with 11.0 ± 0.3 nm (R 2 = 0.9994) deposited per bilayer. The growth behavior of a (chitosan/PGA) n film can range from linear to exponential depending on the polyelectrolyte's charge density during film deposition (51), which contributes significantly to the thickness of film per bilayer (52). When chitosan [pK a ∼6.5 (53)] is well ionized and attains a flat and extended conformation, it demonstrates linear film growth (51), as we observed.…”

Section: Resultssupporting

confidence: 50%

Multimonth controlled small molecule release from biodegradable thin films

Hsu

Park

Hagerman

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Long-term, localized delivery of small molecules from a biodegradable thin film is challenging owing to their low molecular weight and poor charge density. Accomplishing highly extended controlled release can facilitate high therapeutic levels in specific regions of the body while significantly reducing the toxicity to vital organs typically caused by systemic administration and decreasing the need for medical intervention because of its longlasting release. Also important is the ability to achieve high drug loadings in thin film coatings to allow incorporation of significant drug amounts on implant surfaces. Here we report a sustained release formulation for small molecules based on a soluble charged polymer-drug conjugate that is immobilized into nanoscale, conformal, layer-by-layer assembled films applicable to a variety of substrate surfaces. We measured a highly predictable sustained drug release from a polymer thin film coating of 0.5-2.7 μm that continued for more than 14 mo with physiologically relevant drug concentrations, providing an important drug delivery advance. We demonstrated this effect with a potent small molecule nonsteroidal anti-inflammatory drug, diclofenac, because this drug can be used to address chronic pain, osteoarthritis, and a range of other critical medical issues.NSAID | polyelectrolyte multilayers | polymer prodrug

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Section: Resultssupporting

confidence: 50%

Multimonth controlled small molecule release from biodegradable thin films

Hsu

Park

Hagerman

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…(CHI/PGA) films promoted the attachment and growth of C2C12 myoblast cells. [180] So far, it appears that there is limited understanding of the detailed molecular mechanisms of cell adhesion on to these biomimetic films. In particular, there is only a study that aimed to characterize the types of integrins involved in cell adhesion, PSS/ PAH films being taken as substrate.…”

Section: Extracellular Matrix Components As Building Blocksmentioning

confidence: 99%

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Boudou¹,

Crouzier²,

Ren³

et al. 2010

Advanced Materials

679

701

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The design of advanced functional materials with nanometer- and micrometer-scale control over their properties is of considerable interest for both fundamental and applied studies because of the many potential applications for these materials in the fields of biomedical materials, tissue engineering, and regenerative medicine. The layer-by-layer deposition technique introduced in the early 1990s by Decher, Moehwald, and Lvov is a versatile technique, which has attracted an increasing number of researchers in recent years due to its wide range of advantages for biomedical applications: ease of preparation under "mild" conditions compatible with physiological media, capability of incorporating bioactive molecules, extra-cellular matrix components and biopolymers in the films, tunable mechanical properties, and spatio-temporal control over film organization. The last few years have seen a significant increase in reports exploring the possibilities offered by diffusing molecules into films to control their internal structures or design "reservoirs," as well as control their mechanical properties. Such properties, associated with the chemical properties of films, are particularly important for designing biomedical devices that contain bioactive molecules. In this review, we highlight recent work on designing and controlling film properties at the nanometer and micrometer scales with a view to developing new biomaterial coatings, tissue engineered constructs that could mimic in vivo cellular microenvironments, and stem cell "niches."

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“…21 Likewise, the surface properties of ordinary materials can be converted by coating. For example, the surface wettability of materials can be converted by coating with polyelectrolyte complexes, 22 suitable environments for living cells can be created by the polyelectrolyte complex coating for cell culture, 23,24 and protein adsorption can be regulated by the coatings. [25][26][27] Electrostatic interaction between membranes and proteins influences the degree of adsorption of the protein; electrostatic attraction causes an increase in adsorption, 25 whereas electrostatic repulsion decreases the protein adsorption on the membrane.…”

Section: ·2·2 Coating Materialsmentioning

confidence: 99%

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Yabuki

2011

ANAL. SCI.

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The immobilization of biomolecules is an important technique for bio-analysis, and can be applied to biosensors with both high selectivity and high sensitivity. Many researchers have developed immobilization techniques to optimize these characteristics. In the last two decades, an immobilization technique that meets the desired requirements was developed by using polyelectrolytes to form complexes, based on the electrostatic binding between polycations and polyanions. This review summarizes the techniques used for the immobilization of biomolecules by polyelectrolyte complexes; it also discusses related subjects.

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Layer‐by‐Layer Buildup of Poly(L‐glutamic acid)/Chitosan Film for Biologically Active Coating

Cited by 71 publications

References 55 publications

Multimonth controlled small molecule release from biodegradable thin films

Multimonth controlled small molecule release from biodegradable thin films

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

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