Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Yabuki, Soichi

doi:10.2116/analsci.27.695

Cited by 33 publications

(30 citation statements)

References 82 publications

(81 reference statements)

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“…56 Yabuki has recently discussed the size exclusion effects of polyion complex (PIC) films that were prepared by mixing polycation and polyanion solutions. 58 The PIC films composed of poly(L-lysine) and PSS exhibited size exclusion for organic compounds with a molecular weight higher than ~100, and it was H2O2-permeable. 59 Recently, polysaccharide-based LbL films have attracted attention in the development of biosensors and drug-delivery systems because of their biocompatibility and high swellability.…”

Section: ·1 Electrostatic Bonded Lbl Filmsmentioning

confidence: 96%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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We present an overview of the preparation and properties of layer-by-layer (LbL) deposited thin films and microcapsules in relation to their use in the development of biosensors and controlled release systems. Enzyme biosensors can be constructed by immobilizing enzymes on the surface of electrodes by LbL deposition without loss of their catalytic activity. In addition to synthetic polymers, binding proteins, such as avidin and lectin, are also used for constructing LbL films through avidin-biotin and lectin-sugar interactions. The performance characteristics of LbL film-based biosensors can be tuned by controlling the number of layers and by the choice of film components. The permeability of polyelectrolyte LbL films to ions and molecules is discussed in relation to the use of the films for eliminating interference in biosensors. The possible use of polysaccharide LbL film-coated electrodes for the construction of biosensors is highlighted, and examples of LbL film-and microcapsule-based optical sensors are described. We then focus on the use of LbL films and microcapsules as vehicles for controlled release, in particular on recent progress in the controlled release of insulin from LbL films and microcapsules. LbL films composed of insulin and polymers are sensitive to environmental pH, and release insulin in response to pH changes, suggesting that insulin LbL films can be used in the development of orally administered insulin. The construction of glucose-dependent insulin release systems using LbL insulin microcapsules functionalized with phenylboronic acid, lectin, and glucose oxidase is also examined.

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Section: ·1 Electrostatic Bonded Lbl Filmsmentioning

confidence: 96%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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“…24,25 Our group has applied the technique of complex formation to prepare enzyme membranes (polyion complex membrane). The detailed preparation method of enzyme membranes and unique permselective characteristics of the membranes were presented in a previous review, and items related to our previous studies were cited in this review.…”

Section: Polyion Complex Membranesmentioning

confidence: 99%

“…The detailed preparation method of enzyme membranes and unique permselective characteristics of the membranes were presented in a previous review, and items related to our previous studies were cited in this review. 24 One of the advantages of polyion complex membranes is that once the complex is formed, the membrane is very stable. 26 This is because it is formed between polyanions and polycations molecular chains; if the electrostatic binding were composed of only one site, the binding would be easily reversed by association and dissociation, which would alternately occur at one site of the electrostatic binding.…”

Section: Polyion Complex Membranesmentioning

confidence: 99%

Supporting Materials That Improve the Stability of Enzyme Membranes

Yabuki

2014

ANAL. SCI.

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Long-term stability is a key property of enzyme membranes that can be used for biosensors, bioreactors, and bio-fuel cells. This review discusses factors that decrease the stability, and provides two examples of enzyme membranes, a polyion complex membrane and a cellulose membrane, with which stability loss can be avoided. By using these materials, long-term stability was improved. These supporting materials could be applied to construct biosensors, bioreactors, and bio-fuel cells.

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“…Polyelectrolyte complex membranes are divided into two groups based on structure: layer-bylayer films [103,104] and polyion complex membranes [105]. Membranes obtained by the layer-bylayer method are prepared by stacking layers of cationic polyelectrolyte and anionic polyelectrolyte, alternately.…”

Section: Prevention Of Fouling By Supporting Materialsmentioning

confidence: 99%

How to Lengthen the Long-Term Stability of Enzyme Membranes: Trends and Strategies

Yabuki

2017

Catalysts

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Abstract:In this review, factors that contribute to enhancing the stability of immobilized enzyme membranes have been indicated, and the solutions to each factor, based on examples, are discussed. The factors are divided into two categories: one is dependent on the improvement of enzyme properties, and the other, on the development of supporting materials. Improvement of an enzyme itself would effectively improve its properties. However, some novel materials or novel preparation methods are required for improving the properties of supporting materials. Examples have been provided principally aimed at improvements in membrane stability.

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Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Cited by 33 publications

References 82 publications

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Supporting Materials That Improve the Stability of Enzyme Membranes

How to Lengthen the Long-Term Stability of Enzyme Membranes: Trends and Strategies

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