Layer-by-Layer Assembled Polypeptide Capsules for Platinum-Based Pro-Drug Delivery

Abstract

Platinum(IV), a pro-drug of platinum(II), was conjugated to poly(l-lysine) (PLL), and then assembled with poly(glutamic acid) (PGA) through a layer-by-layer (LbL) approach on colloidal silica templates. After removal of the templates, biodegradable PGA/PLL-Pt(IV) multilayer capsules (diameter = 0.5 μm) with 10 μg of platinum incorporated into each bilayer were obtained. Under acidic and/or reductive conditions, the amount and rate of platinum released from the capsules were increased, which are desirable traits for platinum-based anticancer drug delivery systems. Furthermore, in vitro evaluation showed that the PGA/PLL-Pt(IV) multilayer microcapsules displayed higher cytotoxicity (IC(50Pt) = 3.5 μg/mL) against colon cancer cells CT-26 than that of free cisplatin (IC(50Pt) = 8.6 μg/mL). This enhanced cytotoxicity was attributed to the effective internalization of the capsules by the cancer cells, which was observed by confocal laser scanning microscopy (CLSM) imaging.

Recent Advances in Platinum (IV) Complex‐Based Delivery Systems to Improve Platinum (II) Anticancer Therapy

“…). After removal of the templates, PGA/PLL‐Pt (IV) multilayer capsules with 10 μg of platinum incorporated into each bilayer were obtained . The release profiles of platinum from the capsules depended on pH and reducing conditions.…”

“…The release profiles of platinum from the capsules depended on pH and reducing conditions. Furthermore, in vitro cytotoxicity study showed that PGA/PLL‐Pt (IV) multilayer microcapsules were more effective than cisplatin in decreasing viability of CT‐26 colon cancer cells …”

“…After removal of the templates, PGA/PLL-Pt (IV) multilayer capsules with 10 μg of platinum incorporated into each bilayer were obtained. 137 The release profiles of platinum from the capsules depended on pH and reducing conditions. Furthermore, in vitro cytotoxicity study showed that PGA/PLL-Pt (IV) multilayer microcapsules were more effective than cisplatin in decreasing viability of CT-26 colon cancer cells.…”

Functional fluorescent nonporous silica nanoparticles as carriers for Pt(IV) anticancer prodrugs

“…Pt(IV) prodrugs have been tethered to carbon nanotubes [15,33], micelles [34,35], polylactide or poly(D,L-lactic-co-glycolic acid) derivatives [36][37][38], poly(L-lysine) and poly(glutamic acid) derivatives [39]. Fluorescent MSNPs (having hemispherical shape with size 70 × 100 nm) functionalized with (3-aminopropyl)triethoxysilane, have been recently used to carry (OC-6-33)-diamminebis(4-carboxybutanoato) dichloridoplatinum(IV), 1, containing the equatorial arrangement of cisplatin (i.e.…”

Sistema lipossomal de ftalocianina de cloro-alumínio, contendo ácido fólico, aplicada à terapia fotodinâmica

“…Estes vetores podem estar ligados covalentemente à molécula ou associados aos seus respectivos sistemas de veiculação (DDS) ou ainda podem ser introduzidos através de modificações no tecido alvo (ZHOU et al, 2012;XIN et al, 2012;ASAI, 2012).…”