Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

Abstract

BackgroundThe present study was focused on the optimization of yield of the essential oil extraction from leaves of Lawsonia inermis, and the determination of chemical composition, antioxidant activities, and lipid peroxydation and antiproliferative effects.MethodsHenna essential oil (HeEO) were extracted by hydrodistillation; the identification of the chemical composition were done by GC/MS method. HeEO was analyzed for antioxidant power in: (1) chemical system by the DPPH test, the ABTS test and the total antioxidant activity test; and (2) in biological system by lipid peroxydation tests (MDA and DC) in cells culture. The cytotoxicity effects of HeEO were assessed using MTT assay against Raji and HeLa cell lines.ResultsThe optimal extraction yield was 6.8 g/100 g d.b. HeEO showed a remarkable anti-oxidant activities including DDPH (42%), ABTS (87%) and the power of ammonium phosphomolybdate (2992 ± 230 mg of HeEO by equivalent to 1 mg of vitamin C in terms of total antioxidant power).ConclusionBeyond notable antioxidant activities of the HeEo, our results showed a significant decrease in the production of ERO in the Raji cell line. The anti-tumor power of the Henna essential oil shows an interesting cytotoxicity effect (IC50 at 0.26 μg/mL for Raji and at 1.43 μg/mL for HeLa) with a total mortality percentage reaching 60%, for both.

Optimization of Cinnamon (Cinnamomum zeylanicum Blume) Essential Oil Extraction: Evaluation of Antioxidant and Antiproliferative Effects

“…is behavior was observed previously in essential oil extraction from lemongrass (Cymbopogon flexuosus) [10], from Carex meyeriana [30], and from Lawsonia inermis [11] using hydrodistillation.…”

“…Actually, medicinal plants can be used in the treatment of several diseases [8][9][10][11], and a considerable number of essential oils are considered as promising natural compounds, thanks to their different biological activities (e.g., antibacterial, antifungal, antiviral, antioxidant, anticancer, immunomodulatory, analgesic, and anti-inflammatory actions) [12,13].…”

Essential Oils and Mono/bi/tri-Metallic Nanocomposites as Alternative Sources of Antimicrobial Agents to Combat Multidrug-Resistant Pathogenic Microorganisms: An Overview

“…On the other hand, natural products present in medicinal plants like EOs shows significant antioxidants performance from different plant source. The EOs obtained from Zanthoxylum alatum, Ammodaucus leucotrichus, Marrubium globosum, Citrus sinensis and Citrus latifolia, Lawsonia inermis, Thymus fontanesii, Artemisia herba-alba and Rosmarinus officinalis, Syzygium aromaticum L., Origanum vulgare L., Mentha spicata L., and Eremanthus erythropappus M showed promising antioxidant activity [132][133][134][135][136][137].…”

Exploring the Potential of Natural Product-Based Nanomedicine for Maintaining Oral Health

“…Considering the toxicity of anticancer agents coupled with the emergence of resistance, it has become imperative to search for low-risk therapies for cancer treatment [ 32 ]. Studies have suggested that Lawsonia inermis essential oil has the potential to be used as an adjuvant in cancer treatment [ 33 ]. In another study, a cocktail of extracts of Ganoderma lucidum , Antrodia camphorata , and Antler showed an IC50 of 15 mg in 72 h during an MTT assay.…”