Silver nanoparticles (AgNPs) are popular consumer product additives due to their well-known antimicrobial properties. Nowadays, their usage has increased after the emergence of green synthesis method with less toxicity than conventional methods. In this study, we aimed to reveal the antimicrobial potential of phytosynthesized AgNP by Laurus nobilis L. extracts against different Gram-positive, Gram-negative bacteria as well as Candida albicans ATCC 10231. Phyto-synthesized AgNPs were characterized by scanning electron microscopy, zeta size-potential analysis, UV-Visible spectroscopy and FT-IR analysis. The results showed that the size of AgNP was 50 nm and coated by phyto-constituents such as phenolic compounds according to FT-IR results. The antimicrobial activity of AgNP was determined by MIC and MBC tests. The results pointed that green synthesized AgNPs are effective against various microorganisms.