Latex agglutination using the periplasmic proteins antigen of Brucella melitensis is a successful, rapid, and specific serodiagnostic test for ovine brucellosis

Abstract

Brucellosis, especially caused by Brucella melitensis, is considered the most-widespread zoonosis in the world, particularly in developing countries. This study was planned to develop an accurate test for diagnosis of ovine brucellosis using a specific hot saline extracted soluble Brucella melitensis periplasmic proteins (SBPPs). The efficacy of the latex agglutination test (LAT) using SBPPs compared to the Rose Bengal test (RBT), buffered plate agglutination test (BPAT), serum agglutination test (SAT), and an indirect enzyme-linked immunosorbent assay (i-ELISA) was evaluated in the field diagnosis of ovine brucellosis. The test performance was evaluated by estimating sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), disease prevalence (DP), positive likelihood ratio (PLR), and negative likelihood ratio (NLR) using test agreement and bacteriological culture in 1777 samples. The false-positive result was significantly (P ⩽0.05) lower in LAT than RBT, BPAT, SAT, and i-ELISA. With reference to test agreement, the Se, Sp, PPV, and PLR were highest (P ⩽0.05) in LAT 99.33%, 99.88%, 98.68%, and 827.25%, respectively. With reference to bacteriological culture, the LAT and i-ELISA tests showed a significant difference in Se with SAT. However, no significant difference in specificity was detected. The DP was 8.44% in the five tests. In conclusion, LAT using SBPPs of B. melitensis could be a suitable serodiagnostic field test for ovine brucellosis, with high sensitivity and specificity.

A rapid direct-differential agglutination assay for Brucella detection using antibodies conjugated with functionalized gold nanoparticles

“…Frontiers in Nanotechnology frontiersin.org coated latex beads for Brucella canis identification and tube agglutinin testing (TAT) using antigenic solutions of safranin stained Brucella canis were reported (Kimura et al, 2008;Castillo et al, 2014). Latex agglutination testing (LAT) with soluble Brucella melitensis periplasmic proteins (SBPPs) for the detection of ovine brucellosis offered better efficacy over the buffered plate agglutination test (BPAT), RBT, SAT, and I-ELISA, but they are less specific with a high rate of false-positives (Ismael et al, 2016). A comparison between the sero-agglutination test (SAT), Coombs anti-Brucella test, and brucellaCapt test revealed high sensitivity with brucellaCapt, but it also lacks specificity at lower diagnostic titers (Orduna et al, 2000).…”

Pestivirus Infection Does Not Affect Prevalence of Brucella melitensis and Encephalitozoon cuniculi in Small Ruminants of the State of Nuevo Leon, Mexico

“…The hemagglutination Rose Bengal test was used to detect B. melitensis antibodies in serum [17]. Antigen stained with Rose Bengal was used and buffered at a low pH; 25 μl of serum samples were placed in a plate, along with 25 μl of antigen.…”