Lateral resolution enhancement with standing evanescent waves

Cragg, George E.; So, Peter T. C.

doi:10.1364/ol.25.000046

Cited by 127 publications

(77 citation statements)

References 12 publications

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“…Remarkable advances in optical microscopy, including image deconvolution (1,2), multiphoton excitation (3), standingwave techniques (4)(5)(6)(7), and point-spread function shaping (8,9) have pushed the limits of resolution in ideal cases to 70 nm, substantially below the diffraction limit. This limit is still larger than the dimensions of many macromolecular assemblies.…”

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confidence: 99%

High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast

Ajo‐Franklin

Kam

Boxer

2001

Proc. Natl. Acad. Sci. U.S.A.

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Total internal reflection fluorescence microscopy is widely used to confine the excitation of a complex fluorescent sample very close to the material on which it is supported. By working with high refractive index solid supports, it is possible to confine even further the evanescent field, and by varying the angle of incidence, to obtain quantitative information on the distance of the fluorescent object from the surface. We report the fabrication of hybrid surfaces consisting of nm layers of SiO 2 on lithium niobate (LiNbO3, n ‫؍‬ 2.3). Supported lipid bilayer membranes can be assembled and patterned on these hybrid surfaces as on conventional glass. By varying the angle of incidence of the excitation light, we are able to obtain fluorescent contrast between 40-nm fluorescent beads tethered to a supported bilayer and fluorescently labeled protein printed on the surface, which differ in vertical position by only tens of nm. Preliminary experiments that test theoretical models for the fluorescence-collection factor near a high refractive index surface are presented, and this factor is incorporated into a semiquantitative model used to predict the contrast of the 40-nm bead͞ protein system. These results demonstrate that it should be possible to profile the vertical location of fluorophores on the nm distance scale in real time, opening the possibility of many experiments at the interface between supported membranes and living cells. Improvements in materials and optical techniques are outlined.

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confidence: 99%

High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast

Ajo‐Franklin

Kam

Boxer

2001

Proc. Natl. Acad. Sci. U.S.A.

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“…A first practical implementation of this concept was published by Heintzmann and Cremer (1999), and later Gustafsson (2000) and Frohn et al (2000) independently demonstrated microscope set-ups reaching twice the optical resolution. So and co-workers recognized the possibility to further extend the lateral resolution by applying standing wave illumination to total internal reflection fluorescence (TIRF) microscopy (Cragg and So 2000;Chung et al 2006Chung et al , 2007.…”

Section: Developments Toward Extended Resolutionmentioning

confidence: 99%

“…The benefits of combining TIRF microscopy with HELM (TIRF-HELM) have been early recognized by So and coworkers (Cragg and So 2000;Chung et al 2006Chung et al , 2007. In TIRF-HELM, the standing wave is formed by counterpropagating laser beams that experience total internal reflection inside the glass coverslip.…”

Section: Tirf-helmmentioning

confidence: 99%

Widefield fluorescence microscopy with extended resolution

Stemmer

Beck

Fiolka

2008

Histochem Cell Biol

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Widefield fluorescence microscopy is seeing dramatic improvements in resolution, reaching today 100 nm in all three dimensions. This gain in resolution is achieved by dispensing with uniform Köhler illumination. Instead, non-uniform excitation light patterns with sinusoidal intensity variations in one, two, or three dimensions are applied combined with powerful image reconstruction techniques. Taking advantage of non-linear fluorophore response to the excitation field, the resolution can be further improved down to several 10 nm. In this review article, we describe the image formation in the microscope and computational reconstruction of the high-resolution dataset when exciting the specimen with a harmonic light pattern conveniently generated by interfering laser beams forming standing waves. We will also discuss extensions to total internal reflection microscopy, non-linear microscopy, and three-dimensional imaging.

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“…Such fine, detailed information is lost because light emerges with these fine features as evanescent energy, which decays exponentially away from the object and is not carried by the propagating waves. Evanescent energy can be used for imaging to achieve a resolution that is higher than the Abbe limit (1,2). As an example, Lerosey et al (3) demonstrated that subwavelength (that is, super-resolution) imaging of the source location could be achieved by refocusing scattered microwave energy from near-field scatterers.…”

Section: Introductionmentioning

confidence: 99%

Far-field superresolution by imaging of resonant multiples

Schuster

Huang

2014

Geophysical Journal International

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We demonstrate for the first time that seismic resonant multiples, usually considered as noise, can be used for super-resolution imaging in the far-field region of sources and receivers. Tests with both synthetic data and field data show that resonant multiples can image reflector boundaries with resolutions more than twice the classical resolution limit. Resolution increases with the order of the resonant multiples. This procedure has important applications in earthquake and exploration seismology, radar, sonar, LIDAR (light detection and ranging), and ultrasound imaging, where the multiples can be used to make high-resolution images.

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Lateral resolution enhancement with standing evanescent waves

Cited by 127 publications

References 12 publications

High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast

High refractive index substrates for fluorescence microscopy of biological interfaces with high z contrast

Widefield fluorescence microscopy with extended resolution

Far-field superresolution by imaging of resonant multiples

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