Lateral Hypothalamic GABAergic neurons encode and potentiate sucrose’s palatability

Garcia, Aketzali; Coss, Alam; Luis-Islas, Jorge; Purón-Sierra, Liliana; Luna, Monica; Villavicencio, Miguel; Gutiérrez, Ranier

doi:10.1101/2020.06.30.180646

Cited by 3 publications

(4 citation statements)

References 62 publications

(99 reference statements)

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“…In addition to the operant conditioning experiments, our system can facilitate multi-solution briefaccess experiments for studying consummatory behavior. In our task, mice show consumption of a gradient of sucrose, quinine, and NaCl concentrations that closely matches behavior with the freely moving version of the task (Corbit and Luschei, 1969;Coss et al, 2022;Garcia et al, 2021;Glendinning et al, 2002;John et al, 1994;Loney and Meyer, 2018;Smith et al, 1992;Villavicencio et al, 2018).…”

Section: Discussionsupporting

confidence: 61%

“…In our task, mice show consumption of a gradient of sucrose, quinine, and NaCl concentrations that closely matches behavior with the freely moving version of the task (Corbit and Luschei, 1969; Coss et al, 2022; Garcia et al, 2021; Glendinning et al, 2002; John et al, 1994; Loney and Meyer, 2018; Smith et al, 1992; Villavicencio et al, 2018). Licking increased monotonically with increased concentrations of sucrose across all homeostatic states (Garcia et al, 2021; Glendinning et al, 2002; Smith et al, 1992; Spector et al, 1998). However, homeostatic demand states produced pronounced differences in the range of consumption behavior across sucrose concentration, as food-restriction produced a substantially larger range of licking behavior compared to water-restriction.…”

Section: Discussionsupporting

confidence: 54%

“…Licking increased monotonically with increased concentrations of sucrose across all homeostatic states (Garcia et al, 2021;Glendinning et al, 2002;Smith et al, 1992;Spector et al, 1998). However, homeostatic demand states produced pronounced differences in the range of consumption behavior across sucrose concentration, as food-restriction produced a substantially larger range of licking behavior compared to water-restriction.…”

Section: Discussionmentioning

confidence: 99%

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An Open-Source Platform for Head-Fixed Operant and Consummatory Behavior

Gordon-Fennell

Barbakh

Utley

et al. 2023

Preprint

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Head-fixed behavioral experiments in rodents permit unparalleled experimental control, precise measurement of behavior, and concurrent modulation and measurement of neural activity. Here we present OHRBETS (Open-Source Head-fixed Rodent Behavioral Experimental Training System; pronounced Orbitz), a low-cost, open-source ecosystem of hardware and software to flexibly pursue the neural basis of a variety of motivated behaviors. Head-fixed mice tested with OHRBETS displayed operant conditioning for caloric reward that replicates core behavioral phenotypes observed during freely moving conditions. OHRBETS also permits for optogenetic intracranial self-stimulation under positive or negative operant conditioning procedures and real-time place preference behavior, like that observed in freely moving assays. In a multi-spout brief-access consumption task, mice displayed licking as a function of concentration of sucrose, quinine, and sodium chloride, with licking modulated by homeostatic or circadian influences. Finally, to highlight the functionality of OHRBETS, we measured mesolimbic dopamine signals during the multi-spout brief-access task that display strong correlations with relative solution value and magnitude of consumption. All designs, programs, and instructions are provided freely online. This customizable ecosystem enables replicable operant and consummatory behaviors and can be incorporated with methods to perturb and record neural dynamics in vivo.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

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An Open-Source Platform for Head-Fixed Operant and Consummatory Behavior

Gordon-Fennell

Barbakh

Utley

et al. 2023

Preprint

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“…We also thank Ricardo Gaxiola, Victor Manuel García Gómez, and Fabiola Hernandez Olvera for invaluable animal care. This manuscript has been released as a preprint at Biorxiv (Garcia et al, 2020 ).…”

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confidence: 99%

Lateral Hypothalamic GABAergic Neurons Encode and Potentiate Sucrose's Palatability

et al. 2021

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Sucrose is attractive to most species in the animal kingdom, not only because it induces a sweet taste sensation but also for its positive palatability (i.e., oromotor responses elicited by increasing sucrose concentrations). Although palatability is such an important sensory attribute, it is currently unknown which cell types encode and modulate sucrose's palatability. Studies in mice have shown that activation of GABAergic LHAVgat+ neurons evokes voracious eating; however, it is not known whether these neurons would be driving consumption by increasing palatability. Using optrode recordings, we measured sucrose's palatability while VGAT-ChR2 transgenic mice performed a brief access sucrose test. We found that a subpopulation of LHAVgat+ neurons encodes palatability by increasing (or decreasing) their activity as a function of the increment in licking responses evoked by sucrose concentrations. Optogenetic gain of function experiments, where mice were able to choose among available water, 3% and 18% sucrose solutions, uncovered that opto-stimulation of LHAVgat+ neurons consistently promoted higher intake of the most palatable stimulus (18% sucrose). In contrast, if they self-stimulated near the less palatable stimulus, some VGAT-ChR2 mice preferred water over 18% sucrose. Unexpectedly, activation of LHAVgat+ neurons increased quinine intake but only during water deprivation, since in sated animals, they failed to promote quinine intake or tolerate an aversive stimulus. Conversely, these neurons promoted overconsumption of sucrose when it was the nearest stimulus. Also, experiments with solid foods further confirmed that these neurons increased food interaction time with the most palatable food available. We conclude that LHAVgat+ neurons increase the drive to consume, but it is potentiated by the palatability and proximity of the tastant.

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Lateral Hypothalamic GABAergic neurons encode and potentiate sucrose’s palatability

Garcia

Coss

Luis-Islas

et al. 2020

Preprint

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AbstractOverconsumption of calorie-dense palatable foods, especially those with added sugars, is a contributing factor in the obesity epidemic. Although previous studies in mice have shown that GABAergic LHAVgat+ neurons evoke voracious eating, it is not known whether these neurons would be driving consumption by increasing palatability. Using optrode recordings, we measure sucrose’s palatability while VGAT-ChR2 transgenic mice performed a brief access sucrose test. We found that a subpopulation of LHAVgat+ neurons encodes palatability responses elicited by sucrose and that opto-stimulation of these neurons bias the intake towards the most palatable available tastant. In contrast, opto-self-stimulation of LHAVgat+ neurons convey a rewarding signal capable of enhancing the hedonic value of proximal stimuli. We conclude that LHAVgat+ neurons increase consumption by potentiating the palatability of the most proximal tastant available. These findings suggest that LHA GABAergic neurons could be a pharmacological target to modulate the palatability of caloric foods that produce obesity.Impact StatementSucrose is attractive due to its sweet taste and positive palatability. LHA GABAergic neurons encode sucrose’s palatability and act by increasing consumption of the most palatable and proximal tastant available.

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Lateral Hypothalamic GABAergic neurons encode and potentiate sucrose’s palatability

Cited by 3 publications

References 62 publications

An Open-Source Platform for Head-Fixed Operant and Consummatory Behavior

An Open-Source Platform for Head-Fixed Operant and Consummatory Behavior

Lateral Hypothalamic GABAergic Neurons Encode and Potentiate Sucrose's Palatability

Lateral Hypothalamic GABAergic neurons encode and potentiate sucrose’s palatability

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