Lateral fluid flow fractionation using dielectrophoresis (LFFF-DEP) for size-independent, label-free isolation of circulating tumor cells

Waheed, Waqas; Alazzam, Anas; Mathew, Bobby; Christoforou, Nicolas; Abu‐Nada, Eiyad

doi:10.1016/j.jchromb.2018.04.046

Cited by 58 publications

(52 citation statements)

References 17 publications

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“…For example, the flow rate was about 0.3 μL/min for a work using electrodes embedded on a channel side wall [20] or the throughput was 8.4 × 10 4 cells/min for contactless separation [31], which also needed rather high voltages for operation. The throughput of device was limited due to the low flow rate [32,33], which was due to channel dimensions. It is worth noting that with embedded electrodes on a side wall of a microchannel, the channel width is restricted by the region of nonuniform field, which is defined by the electrode gap.…”

Section: Introductionmentioning

confidence: 99%

Study on the discrete dielectrophoresis for particle–cell separation

et al. 2020

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Study on the discrete dielectrophoresis for particle-cell separationThis paper presents the application of the discrete dielectrophoretic force to separate polystyrene particles from red blood cells. The separation process employs a simple microfluidic device that is composed of interdigitated electrodes and a microchannel. The discrete dielectrophoretic force is generated by adjusting the duty cycle of the applied voltage. The electrodes make a tilt angle with the microchannel to change the moving direction of the red blood cells. By adjusting the voltage magnitude and duty cycle, we investigate the deflection of red blood cells and the variation of cell velocity along electrode edge under positive dielectrophoresis. The experiments with polystyrene particles show that the enrichment of the particles is greater than 150 times. The maximum separation efficiency is 97% for particle-to-cell number ratio equal to 1:2000 in the sample having high cell concentration. Using the appropriate applied voltage magnitude and duty cycle, the discrete dielectrophoretic force can prevent the clogging of microchannel while successfully separating the particles from the cells with high enrichment and efficiency. The proposed principle can be readily applied to dielectrophoresis-based devices for biomedical sample preparation or diagnosis such as the separation of rare or infected cells from a blood sample.

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Section: Introductionmentioning

confidence: 99%

Study on the discrete dielectrophoresis for particle–cell separation

et al. 2020

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“…Additionally, DEP may be successfully combined with other separation approaches like inertial force microfluidic separation [223], fluid flow fractionation [224], and field-modulated electroosmotic flow separation [225].…”

Section: Electrokinetic Cell Separationmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…The first practical method to counteract the size-dependence of DEP was demonstrated by Wang, et al [12], and used gravity to perform a combination of DEP and field flow fractionation (FFF). Subsequently, the process has been refined and separations of differentiated stem cells, [13], circulating tumor cells [14,15] and bacteria [16] have been demonstrated.…”

Section: Gravity-based Systems: Dep-fffmentioning

confidence: 99%

High-Sensitivity in Dielectrophoresis Separations

2020

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The applications of dielectrophoretic (DEP) techniques for the manipulation of cells in a label-free fashion within microfluidic systems continue to grow. However, a limited number of methods exist for making highly sensitive separations that can isolate subtle phenotypic differences within a population of cells. This paper explores efforts to leverage that most compelling aspect of DEP—an actuation force that depends on particle electrical properties—in the background of phenotypic variations in cell size. Several promising approaches, centering around the application of multiple electric fields with spatially mapped magnitude and/or frequencies, are expanding the capability of DEP cell separation.

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Lateral fluid flow fractionation using dielectrophoresis (LFFF-DEP) for size-independent, label-free isolation of circulating tumor cells

Cited by 58 publications

References 17 publications

Study on the discrete dielectrophoresis for particle–cell separation

Study on the discrete dielectrophoresis for particle–cell separation

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

High-Sensitivity in Dielectrophoresis Separations

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