High-Sensitivity in Dielectrophoresis Separations

Hawkins, Benjamin G.; Lai, Nelson; Clague, David S.

doi:10.3390/mi11040391

Cited by 12 publications

(6 citation statements)

References 61 publications

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“…However, in contrast to continuous-flow mode, DEP-FFF is typically operated in batch mode due to its reliance on a prior cell levitation step. Furthermore, separating cells that are equilibrated in multiple streams is challenging, as it is sensitive to variations in device depth and its orientation with respect to the gravity force [55]. Yan et al integrated DEP with hydrophoretic focusing enabled by crescent-shaped grooves and demonstrated highthroughput (~9 mL h −1 ) particles/cells focusing [56].…”

Section: Discussionmentioning

confidence: 99%

A High-Throughput Microfluidic Cell Sorter Using a Three-Dimensional Coupled Hydrodynamic-Dielectrophoretic Pre-Focusing Module

Aghaamoo,

Cardenas-Benitez,

Lee

2023

Micromachines

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Dielectrophoresis (DEP) is a powerful tool for label-free sorting of cells, even those with subtle differences in morphological and dielectric properties. Nevertheless, a major limitation is that most existing DEP techniques can efficiently sort cells only at low throughputs (<1 mL h−1). Here, we demonstrate that the integration of a three-dimensional (3D) coupled hydrodynamic-DEP cell pre-focusing module upstream of the main DEP sorting region enables cell sorting with a 10-fold increase in throughput compared to conventional DEP approaches. To better understand the key principles and requirements for high-throughput cell separation, we present a comprehensive theoretical model to study the scaling of hydrodynamic and electrostatic forces on cells at high flow rate regimes. Based on the model, we show that the critical cell-to-electrode distance needs to be ≤10 µm for efficient cell sorting in our proposed microfluidic platform, especially at flow rates ≥ 1 mL h−1. Based on those findings, a computational fluid dynamics model and particle tracking analysis were developed to find optimum operation parameters (e.g., flow rate ratios and electric fields) of the coupled hydrodynamic-DEP 3D focusing module. Using these optimum parameters, we experimentally demonstrate live/dead K562 cell sorting at rates as high as 10 mL h−1 (>150,000 cells min−1) with 90% separation purity, 85% cell recovery, and no negative impact on cell viability.

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Section: Discussionmentioning

confidence: 99%

A High-Throughput Microfluidic Cell Sorter Using a Three-Dimensional Coupled Hydrodynamic-Dielectrophoretic Pre-Focusing Module

Aghaamoo,

Cardenas-Benitez,

Lee

2023

Micromachines

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“…Solution conductivity is a determining factor for optimal sensitivity that depends on the DEP technique used, but usually low or high conductivity is favored which potentially puts biological samples in harmful conditions that can alter their physiological structure. 79…”

Section: Ev Isolation and Characterization Technologiesmentioning

confidence: 99%

Emerging micro-nanotechnologies for extracellular vesicles in immuno-oncology: from target specific isolations to immunomodulation

2022

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“…Some of the mainstream active methods are dielectrophoresis (DEP), electrophoresis, acoustic separation, optical tweezers, and magnetophoresis [16][17][18]. The DEP method applies an electric field to cells, allowing for their separation based on their electrical properties [19]. Cells with different dielectric properties experience different forces and are separated based on their response to the electric field.…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost Laser-Prototyped Microfluidic Device for Separating Cells and Bacteria

Gucluer,

Guler

2023

Applied Sciences

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Simple and rapid fabrication of microfluidic devices can enable widespread implementation of lab-on-chip devices in resource-limited environments. However, currently most of the microfluidic devices are fabricated in cleanroom facilities that are well-funded and not accessible to most of the researchers in developing countries. Herein, a simple, low-cost, and reliable method is shown to fabricate microfluidic devices for separating cells and bacteria-size microparticles. For this purpose, serpentine and spiral microfluidic channels are designed and fabricated using rapid laser prototyping. This single inlet microfluidic device is shown to successfully separate yeast cells and smaller microparticles with an efficiency of 85% which is very promising for many lab-on-chip applications including cell-based diagnostics and therapeutics.

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High-Sensitivity in Dielectrophoresis Separations

Cited by 12 publications

References 61 publications

A High-Throughput Microfluidic Cell Sorter Using a Three-Dimensional Coupled Hydrodynamic-Dielectrophoretic Pre-Focusing Module

A High-Throughput Microfluidic Cell Sorter Using a Three-Dimensional Coupled Hydrodynamic-Dielectrophoretic Pre-Focusing Module

Emerging micro-nanotechnologies for extracellular vesicles in immuno-oncology: from target specific isolations to immunomodulation

A Low-Cost Laser-Prototyped Microfluidic Device for Separating Cells and Bacteria

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