Lateral flow test for visual detection of multiple MicroRNAs

Zheng, Wanli; Li, Yao; Teng, Jun; Yan, Chao; Qin, Panzhu; Liu, Guodong; Chen, Wei

doi:10.1016/j.snb.2018.02.159

Cited by 88 publications

(48 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Up to now, no perfect method can overcome all shortcomings. However, probe-based lateral flow assay in combination with SDA could provide robust multiplexing detection higher than their peer antibodies [84]. For example, our group [15,24,36] and other researchers [3,100,101] demonstrated that SDA can be used to amplify short target recognition sequences, for example, aptamers, after target binding and then integrated with lateral flow biosensors for analysis.…”

Section: Discussion Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Lateral flow biosensor is the most commonly used technology for the point of care testing [81][82][83][84]. A test strip consists of four parts: a sample pad, a conjugate pad, a nitrocellulose membrane, and an absorption pad.…”

Section: Lateral Flow Biosensormentioning

confidence: 99%

“…This method is fast, specific, sensitive, and cost effective. With different targeting aptamers and corresponding test zones on the test strip, multiplexing assay can be developed to detect multiple pathogens [84].…”

Section: Lateral Flow Biosensormentioning

confidence: 99%

See 2 more Smart Citations

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Zeng¹,

Mukama²,

Lu³

et al. 2019

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

View full text Add to dashboard Cite

The identification of various targets such as bacteria, viruses, and other cells remains a prerequisite for point-of-care diagnostics and biotechnological applications. Nucleic acids, as encoding information for all forms of life, are excellent biomarkers for detecting pathogens, hereditary diseases, and cancers. To date, many techniques have been developed to detect nucleic acids. However, most of them are based on polymerase chain reaction (PCR) technology. These methods are sensitive and robust, but they require expensive instruments and trained personnel. DNA strand displacement amplification is carried out under isothermal conditions and therefore does not need expensive instruments. It is simple, fast, sensitive, specific, and inexpensive. In this chapter, we introduce the principles, methods, and updated applications of DNA strand displacement technology in the detection of infectious diseases. We also discuss how robust, sensitive, and specific nucleic acid detection could be obtained when combined with the novel CRISPR/Cas system.

show abstract

Section: Discussion Conclusion and Future Perspectivesmentioning

confidence: 99%

Section: Lateral Flow Biosensormentioning

confidence: 99%

See 1 more Smart Citation

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Zeng¹,

Mukama²,

Lu³

et al. 2019

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

View full text Add to dashboard Cite

show abstract

“…S13 and Table 1). However, there was not any signal in either test zone or control zone when 50 μL of pure serum samples with miR-210 mimic (up to 50 pmol) was loaded with direct sampling method, possibly because the binding ability between nucleotides is poor in pure serum on the condition that the sample pad has not been pre-treated by buffer solution before usage 31 . In both sandwich-like and competitive formats, LODs obtained by pre-enrichment at 1:1 dilution were equivalent to those obtained in running buffers, indicating that the proposed test-zone pre-enrichment sampling strategy had promising analytical applications in real biological samples.…”

Section: Analysis Of Mock Clinical Samplesmentioning

confidence: 99%

Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment

Zhang

Liu

Wang

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Lateral flow assay (LFA) is one of the most prevalent commercially available techniques for point-of-care tests due to its simplicity, celerity, low cost and robust operation. However, conventional colorimetric LFAs have inferior limits of detection (LODs) compared to sophisticated laboratory-based assays. Here, we report a simple strategy of test-zone pre-enrichment to improve the LoD of LfA by loading samples before the conjugate pad assembly. The developed method enables visual LODs of miR-210 mimic and human chorionic gonadotropin protein, to be improved by 10-100 fold compared with a conventional LfA setup without introducing any additional instrument and reagent except for phosphate running buffer, while no obvious difference occurred for Aflatoxin B1 (AFB1). It takes about 6-8 min to enrich every 50 μL of sample diluted with phosphate running buffer, therefore we can get visual results within 20 min. We identified a parameter by modeling the entire process, the concentration of probe-analyte conjugate at test zone when signaling unit being loaded, to be important for the improvement of visual limit of detection. In addition, the test-zone pre-enrichment did not impair the selectivity when miR-210 mimic was adopted as target. Integrated with other optimization, amplification and modification of LfAs, the developed test-zone pre-enrichment method can be applied to further improve LoD of LfAs. Lateral flow assay (LFA), a paper-based in-situ detection platform, has been widely used in diverse fields such as biomedicine, environmental health, quality control and food safety due to its rapid, inexpensive, portable measurements 1. Basing on affinity interactions such as hybridization of oligonucleotide or aptamer-target, antibody-antigen or biotin-streptavidin, the signal of the test zone on the LFA test strip changes with the concentration of a particular analyte of interest when a liquid sample is applied 2,3. There are two basic formats of LFA: sandwich format and competitive format. In addition, the competitive format could be designed in the following two ways: fixing the capture probe of target at test zone (competitive format I) or fixing the competitive analogue at test zone (competitive format II). Generally, there are two strategies to load signaling unit in the conventional LFA. The first one is the direct sampling method with drying signaling unit on the conjugate pad fixed between the nitrocellulose (NC) membrane and the sample pad before sampling. This strategy is the most widely used and easily handled one and the results usually show significant concentration dependence. However, the limit of detection (LOD) is usually poor, which is the main drawback of this conventional LFA. The other strategy is the pre-mixing/pre-incubation sampling method with mixing signaling unit with sample solution to load together onto the sample pad 4. Signaling unit is greatly diluted by sample solution in this strategy, resulting in a prolonged interaction between signaling unit and capture molecules at test zone and ...

show abstract

“…In light of this, numerous groups have developed strip-based biosensors for miRNA detection (Hou et al, 2012;Gao et al, 2014;Deng et al, 2017a). Given most medical diagnoses are made after testing a panel of two or more biomarkers, Zheng et al (2018) developed a multiplexed lateral flow assay for the simultaneous detection of miR-21, miR-155 and miR-210 in human serum samples at concentrations as low as 68, 7, and 17 pM, respectively, within 10 min ( Figure 3A). Deng et al (2017a) sought to overcome the limited sensitivity of previous lateral flow based biosensors by incorporating a target recycling amplification strategy, whereby two sequence specific hairpins are used to amplify the signal without the need for added enzymes.…”

Section: New and Emerging Mirna Detection Platformsmentioning

confidence: 99%

MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

et al. 2020

View full text Add to dashboard Cite

In the pursuit of improved diagnostic tests for infectious diseases, several classes of molecules have been scrutinized as prospective biomarkers. Small (18-22 nucleotide), non-coding RNA transcripts called microRNAs (miRNAs) have emerged as promising candidates with extensive diagnostic potential, due to their role in numerous diseases, previously established methods for quantitation and their stability within biofluids. Despite efforts to identify, characterize and apply miRNA signatures as diagnostic markers in a range of non-infectious diseases, their application in infectious disease has advanced relatively slowly. Here, we outline the benefits that miRNA biomarkers offer to the diagnosis, management, and treatment of infectious diseases. Investigation of these novel biomarkers could advance the use of personalized medicine in infectious disease treatment, which raises important considerations for validating their use as diagnostic or prognostic markers. Finally, we discuss new and emerging miRNA detection platforms, with a focus on rapid, point-of-care testing, to evaluate the benefits and obstacles of miRNA biomarkers for infectious disease.

show abstract

Lateral flow test for visual detection of multiple MicroRNAs

Cited by 88 publications

References 41 publications

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment

MicroRNA Biomarkers for Infectious Diseases: From Basic Research to Biosensing

Contact Info

Product

Resources

About