Lateral flow immunoassay for progesterone detection

Safronova, V.A.; Samsonova, J.V.; Grigorenko, G.M.; Осипов, А. И.

doi:10.3103/s0027131412050045

Cited by 13 publications

(10 citation statements)

References 17 publications

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“…As it was shown in our previous article colloidal gold label in LFIA of P4 did not provide required assay sensitivity [22]. So the approach called LFEIA (lateral flow enzyme immunoassay) consisting of the combination of the sensitivity of enzyme immunoassay with the rapidity of lateral flow assay was proposed.…”

Section: Resultsmentioning

confidence: 98%

“…Polyclonal rabbit antiserum obtained against 11α-hydroxyprogesterone hemisuccinate and the synthesis of a conjugate of 3-O-carboxymethyl oxime progesterone (3-CMO-P4) with HRP were described elsewhere [22].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work the main approaches to a new P4 test based of lateral flow principle and utilized an enzyme as a label were established [22]. The objective of the present study was to develop quick and sensitive lateral flow enzyme immunoassay (LFEIA) for P4 determination in whole cows' milk without preliminary sample preparation.…”

Section: Introductionmentioning

confidence: 99%

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Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows’ milk

Samsonova

Safronova

Осипов

2015

Talanta

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows’ milk

Samsonova

Safronova

Осипов

2015

Talanta

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“…In these conditions, the luciferase activity did not decrease during 120 min incubation of the reaction mixture at 0°C (under the conjugation conditions). Previously, horseradish peroxidase was conjugated with progesterone in 0.01 m borate buffer (pH 8.6) . However, a significant loss of the luciferase activity was observed during the conjugation performed in these conditions.…”

Section: Resultsmentioning

confidence: 99%

The Bioluminescence Resonance Energy Transfer from Firefly Luciferase to a Synthetic Dye and its Application for the Rapid Homogeneous Immunoassay of Progesterone

Smirnova

Samsonova

Ugarova

2015

Photochem & Photobiology

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The sensitive BRET system for the homogeneous immunoassay of a low-molecular weight antigen was developed using progesterone as an example. Two thermostable mutants of the Luciola mingrelica firefly luciferase (Luc)-the "red" mutant with λmax.em = 590 nm (RedLuc) and the "green" mutant with λmax.em = 550 nm (GreenLuc)-were tested as the donors. The water-soluble Alexa Fluor 610× (AF) dye was selected as the acceptor because its two absorption maxima, located at 550 and 610 nm, are close to the bioluminescence maxima of the GreenLuc and RedLuc, respectively. The methods for the synthesis of the luciferase-progesterone (Luc-Pg) conjugate and the conjugate of the dye and the polyclonal antiprogesterone antibody (AF-Ab) were developed. Both conjugates retained their functional properties, had high antigen-antibody binding activity, and demonstrated a high BRET signal. The homogeneous immunoassay system based on the BRET from the firefly luciferase to the synthetic dye was established to assay progesterone as a model antigen. Optimization of the assay conditions, the composition of the reaction mixture, and the concentrations of the donor and the acceptor made it possible to reach the minimum detectable progesterone concentration of 0.5 ng mL(-1) .

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“…It normally ranges in level from less than 1 ng/ml in serum during the pre-ovulation phase of the menstrual cycle up to 20 ng/mL in mid cycle and to 300+ ng/mL in pregnancy. Immunoassays such as ELISA 8 , electrophoresis-chemiluminescence detection 9 , and non-competitive idiometric or fluoroimmunoassays 10 , have been the most widely used methods for the detection of progesterone in different matrices. These tests require very carefully controlled procedures, skilled experts to carry out the analysis, and labeled probes to detect the steroid.…”

mentioning

confidence: 99%

Nano-SPRi Aptasensor for the Detection of Progesterone in Buffer

Zeidan

Shivaji

Henrich

et al. 2016

Sci Rep

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Progesterone is a steroid hormone that plays a central role in the female reproductive processes such as ovulation and pregnancy with possible effects on other organs as well. The measurement of progesterone levels in bodily fluids can assist in early pregnancy diagnosis and can provide insight for other reproductive functions. In this work, the detection of progesterone was examined by integrating novel aptamer development with a nanoEnhanced surface plasmon resonance imaging sensor. First, we developed X-aptamers and selected them for binding to progesterone. Then, we took advantage of the multi-array feature of SPRi to develop an optimized biosensor capable of simultaneously screening the 9 X-aptamers developed to determine the binding capabilities of each aptamer. The sensor surface design conditions were further optimized for the sandwich assay, which employed nanoEnhancers (NIR-streptavidin coated quantum dots) for ultrasensitive detection of progesterone molecules. The assay designed was examined over a concentration range of 1.575 ng/mL to 126 μg/mL resulting in a limit of detection (LOD) of 1.575 ng/mL (5 nM) in phosphate buffer.

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Lateral flow immunoassay for progesterone detection

Cited by 13 publications

References 17 publications

Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows’ milk

Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows’ milk

The Bioluminescence Resonance Energy Transfer from Firefly Luciferase to a Synthetic Dye and its Application for the Rapid Homogeneous Immunoassay of Progesterone

Nano-SPRi Aptasensor for the Detection of Progesterone in Buffer

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