Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement amplification

Fang, Zhiyuan; Wu, Wei; Lu, Xuewen; Zeng, Lingwen

doi:10.1016/j.bios.2014.01.015

Cited by 161 publications

(94 citation statements)

References 36 publications

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“…But compared with antibodies, aptamers are easier to modify, faster to synthesize, more stable and cheaper. Moreover, aptamers can capture both small molecules (e.g., nucleic acids, nucleotides, peptides, protein) [15] and whole cells by covalent bonding and spatial conformation recognition [16]. So, aptamer-based biosensors, known as aptasensors, possess great advantages with high productivity and selectivity and exhibit remarkable prospects for application in food safety and biological diagnostics [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

et al. 2015

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We describe a Salmonella biosensor that was obtained by electrochemical immobilization of a nanocomposite consisting of reduced graphene oxide (rGO) and carboxymodified multi-walled carbon nanotubes (MWCNTs) directly on the surface of a glassy carbon electrode (GCE). An aminomodified aptamer specific for Salmonella was covalently bound to the rGO-MWCNT composite via amide bonds. The morphology of the rGO-MWCNT nanocomposite was characterized by transmission electron microscopy and scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy were used to monitor all steps during assembly. When exposed to samples containing Salmonella, the anti-Salmonella aptamer on the electrode captures its target. Hence, electron transfer is blocked, and this results in a large increase in impedance. Salmonella can be quantified by this aptasensor, typically operated at a working voltage of 0.2 V (vs. Ag/AgCl), in the range from 75 to 7.5×10 5 cfu⋅mL −1 and detection limit of 25 cfu⋅mL −1 (at an S/N of 3). The method is perceived to have a wide scope in that other bacteria may be detected by analogy to this approach and with very low limits of detection by applying respective analyte-specific aptamers.

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Section: Introductionmentioning

confidence: 99%

Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

et al. 2015

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“…Among the foodborne pathogens related to poisoning, Salmonella is reported to be the leading cause of poisoning in many countries including China [3,4]. Salmonella is a genus of Gram-negative bacteria that has more than 2000 pathogenic serotypes including the well-known serotypes S. typhimurium and S. enteritidis [5,6]. The main antigens of Salmonella are lipopolysaccharide (O antigen), flagellin (H antigen), and capsular polysaccharide (Vi antigen).…”

Section: Introductionmentioning

confidence: 99%

“…However, the dependence on a relatively high temperature (63°C) and DNA extraction step still limit the practical application of these methods [11,12]. Due to the specificity of the selected aptamer, aptamer-based sensors are mainly used for rapid detection of common serotypes such as S. typhimurium and S. enteritidis, rather than detection of the genus [5,13,14]. Immunoassays based on the specific antigen and antibody reaction have been widely used in Salmonella detection either with the classic enzyme-linked immunosorbent assay (ELISA) [15][16][17][18] or novel nanomaterials-based biosensors [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based strip sensor for multiple detection of twelve Salmonella strains with a genus-specific lipopolysaccharide antibody

et al. 2016

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In this study, an innovative competitive immunochromatographic strip sensor was developed for rapid detection of Salmonella based on a genus-specific antilipopolysaccharide (LPS) monoclonal antibody (mAb) and the heterogeneous coating antigen of a LPS-bovine serum albumin conjugate. Gold nanoparticles labeled anti-LPS mAb specifically reacted with the conserved outer core of the Salmonella LPS in the sample and the color formed on the T line was negatively correlated with the number of Salmonella cells. The sensitivity of Ra mutant LPS (without O-specific chains but has the conserved outer core) was 25 ng mL -1 , which explained the detection of Salmonella at the genus level. Based on the gray values on the test line, the limit of detection of Salmonella was 10 3 colony-forming unit (CFU) for all twelve typical strains of Salmonella. The analysis of common Gram-negative and Gram-positive bacteria demonstrated that the strip assay was specific to Salmonella. A milk sample test showed that Salmonella at a low level (1-5 CFU mL -1 ) was detected without complex biochemical confirmation steps, sophisticated instruments and professional training.

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“…This offers high accuracy and sensitivity but is poorly suited for implementation in low-resource settings due to the complicated sample pretreatment, the need for trained technicians, and the three to four week turnaround time (Crannell et al, 2014;Fang et al, 2014;Gao et al, 2014;Rohrman and Richards-Kortum, 2012). Furthermore, false-negative results may occur because the PCR involves a thermo-cyclic amplification process that utilizes minute quantities of DNA.…”

Section: Introductionmentioning

confidence: 99%

“…LF strips are considered to be one of the most promising techniques due to their simplicity, rapid analysis, relatively low interference due to chromatographic separation, low costs, long-term stability over a wide range of climates, and their ability to be easily used with no requirements for skilled technicians (Chen et al, 2013;Mao et al, 2009b;Shen et al, 2013). Until now, several different types of LF strip have been developed to detect nucleic acids including DNA or miRNA (Fang et al, 2014;Gao et al, 2014;He et al, 2010;Hu et al, 2013;Mao et al, 2009b). For example, a dry-reagent strip biosensor based on a molecular beaconfunctionalized gold nanoparticle probe has been developed for rapid and quantitative detection of specific nucleic acids (Mao et al, 2009b).…”

Section: Introductionmentioning

confidence: 99%

A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

Cheng

et al. 2016

Biosensors and Bioelectronics

325

173

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a b s t r a c tUser-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity.

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Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement amplification

Cited by 161 publications

References 36 publications

Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes

Gold nanoparticle-based strip sensor for multiple detection of twelve Salmonella strains with a genus-specific lipopolysaccharide antibody

A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

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