Lateral flow assays

Koczula, Katarzyna M.; Gallotta, Andrea

doi:10.1042/ebc20150012

Cited by 894 publications

(803 citation statements)

References 52 publications

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“…Moreover, without additional hardware, like POCScan reader, semiquantitative or quantitative analysis is not possible. As a variety of biological samples such as serum, plasma, saliva, urine and whole blood can be analysed by LFIA, the assay has been already widely used in the human and veterinary medical diagnostics . The current assay principle is already utilized for the serological detection of platelet factor 4/heparin specific antibodies in thrombocytopenia and the detection of the pro‐inflammatory cytokine interleukin 6 in various body fluids …”

Section: Discussionmentioning

confidence: 99%

A novel lateral flow immunoassay for the rapid detection of anti‐Dsg3 IgG serum autoantibodies in pemphigus vulgaris

Schmidt

Mauracher

Bender

et al. 2018

Experimental Dermatology

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Pemphigus vulgaris (PV) is a severe autoimmune blistering disease of the skin and mucous membranes. As autoantibodies play an essential role in the disease pathogenesis, the serological detection of anti-desmoglein 3 IgG represents a central tool in the diagnosis of the disease. In this study, we show the validation of a novel lateral flow immunoassay (LFIA) which rapidly detects anti-desmoglein 3 (Dsg3) IgG in human serum. In contrast to other diagnostic procedures, the assay is compact and simple to perform and delivers a fast "yes" or "no" answer within 10 minutes without additional hardware requirements for test evaluation. For validation, a blinded collection of 200 sera including 100 sera from 14 PV patients, 75 sera from 24 bullous pemphigoid patients and 25 sera from 6 patients with pemphigus foliaceus collected at different time points during disease was used. Presence or non-presence of anti-Dsg3 IgG within sera was confirmed using a commercially available Dsg3-ELISA. For qualitative evaluation, Dsg3-LFIA test results were assessed by two independent groups of human observers. Furthermore, quantitative evaluation using POCScan reader was applied. The Dsg3-LFIA demonstrated reliable test results with a sensitivity and specificity of 78.1% and 97.1%, respectively. Test results from POCScan and human observers showed a substantial agreement. The Dsg3-LFIA represents a new diagnostic tool for the immediate and reliable detection of anti-desmoglein 3 serum IgG autoantibodies that does not require additional hardware. Further prospective trials are warranted to validate the Dsg3 LFIA in pemphigus.

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Section: Discussionmentioning

confidence: 99%

A novel lateral flow immunoassay for the rapid detection of anti‐Dsg3 IgG serum autoantibodies in pemphigus vulgaris

Schmidt

Mauracher

Bender

et al. 2018

Experimental Dermatology

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“…[109] A liquid sample is distributed by capillary force onto a paper that has zone-specific sectors where the virus can interact, leading to the generation of color zones. [110,111] 10. [110,111] 10.…”

Section: Nanomaterials-based Bioreceptorsmentioning

confidence: 99%

Viral Detection: Past, Present, and Future

et al. 2019

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Viruses are essentially composed of a nucleic acid (segmented or not, DNA, or RNA) and a protein coat. Despite their simplicity, these small pathogens are responsible for significant economic and humanitarian losses that have had dramatic consequences in the course of human history. Since their discovery, scientists have developed different strategies to efficiently detect viruses, using all possible viral features. Viruses shape, proteins, and nucleic acid are used in viral detection. In this review, the development of these techniques, especially for plant and mammalian viruses, their strengths and weaknesses as well as the latest cutting‐edge technologies that may be playing important roles in the years to come are described.

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“…Las intervenciones deben adaptarse a las condiciones locales y considerar la posibilidad de detección y erradicación de H. pylori, en el contexto de evaluación de la viabilidad, la eficacia y las consecuencias adversas (17) .…”

Section: Introductionunclassified

Perfil de valores de estudiantes de carreras de salud del sur de Chile

et al. 2017

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RESUMENObjetivo: Conocer la seroprevalencia de infección por Helicobacter pylori en población adulta de Lima, Perú 2017. Materiales y métodos: Estudio descriptivo, prospectivo, transversal. Población conformada por voluntarios mayores de 18 años, de ambos sexos, con o sin molestias gastroenterológicas generales. Campaña de despistaje realizada en los distritos de Magdalena y Chorrillos de la ciudad de Lima, Perú en el mes de enero del 2017. Para el diagnóstico se utilizó la prueba rápida OnSite H. pylori Ab Combo Rapid Test CE de CTK Biotech. Resultados: Se evaluó a 140 pacientes, edad media 36.6 años, 22.1% de sexo masculino y 77.9% de sexo femenino. La seroprevalencia para Helicobacter pylori fue 63.6%. Conclusiones: Nosotros concluimos que la infección por Helicobacter pylori es frecuente en el área de la ciudad de Lima, sin diferencia entre género y edad.Palabras clave: Helicobacter pylori; cáncer de estómago; gastritis; úlcera gástrica. Seroprevalence of Helicobacter pylori infection among an adult population of Lima, Peru 2017ABSTRACT Objective: To know the seroprevalence of Helicobacter pylori infection among an adult population of Lima, Peru 2017. Materials and methods: Descriptive, prospective, cross-sectional study. Population of volunteers older than 18 years, of both sexes, with or without general gastrointestinal discomfort. A screening campaign was carried out in the districts of Magdalena and Chorrillos in the city of Lima, Peru, in January 2017. For the diagnosis, CTK Biotech's OnSite H. pylori Ab Combo Rapid Test CE was used. Results: One hundred forty (140) patients were evaluated, with a mean age of 36.6 years old, being 22.1% male and 77.9% female. The seroprevalence of Helicobacter pylori infection was 63.6%. Conclusions: We conclude that Helicobacter pylori infection is common in the city of Lima, with no difference between gender and age.

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Lateral flow assays

Cited by 894 publications

References 52 publications

A novel lateral flow immunoassay for the rapid detection of anti‐Dsg3 IgG serum autoantibodies in pemphigus vulgaris

A novel lateral flow immunoassay for the rapid detection of anti‐Dsg3 IgG serum autoantibodies in pemphigus vulgaris

Viral Detection: Past, Present, and Future

Perfil de valores de estudiantes de carreras de salud del sur de Chile

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