Late response to allergen is associated with increased concentrations of tumor necrosis factor-α and IL-5 in induced sputum

Keatings, Vera M.; O’Connor, Brian; Wright, Lyndon G.; Huston, David P.; Corrigan, Christopher; Barnes, Peter J.

doi:10.1016/s0091-6749(97)70032-0

Cited by 95 publications

(75 citation statements)

References 18 publications

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“…TNF␣, either newly synthesized or preformed and stored constitutively within the mast cell granules, is coreleased with other potent mediators such as histamine and tryptase via the IgE-dependent mechanism (9, 21, 47). TNF␣ was detected in bronchoalveolar lavage fluid, sputum, exhaled breath condensate, and serum of asthmatic patients during asthmatic attack or following antigen inhalation challenge (12,33,39). Furthermore, it has been shown that TNF␣ is released from sensitized human and mouse lung tissue in response to antigen challenge (21,33,47).…”

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confidence: 99%

“…TNF␣ was detected in bronchoalveolar lavage fluid, sputum, exhaled breath condensate, and serum of asthmatic patients during asthmatic attack or following antigen inhalation challenge (12,33,39). Furthermore, it has been shown that TNF␣ is released from sensitized human and mouse lung tissue in response to antigen challenge (21,33,47). TNF␣ is known to exert multiple potent effects on a number of effector cells in the airways; for example, it is a chemoattractant for neutrophils and eosinophils, upregulates the leukocyte-endothelial cell adhesive molecule E-selectin, enhances the production of TH2 cytokines, and increases microvascular permeability (8,34,48).…”

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confidence: 99%

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Calcium transient evoked by TRPV1 activators is enhanced by tumor necrosis factor-α in rat pulmonary sensory neurons

Gu²,

Lin³

et al. 2010

American Journal of Physiology-Lung Cellular and Molecular Phys

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Hu Y, Gu Q, Lin RL, Kryscio R, Lee LY. Calcium transient evoked by TRPV1 activators is enhanced by tumor necrosis factor-␣ in rat pulmonary sensory neurons. Am J Physiol Lung Cell Mol Physiol 299: L483-L492, 2010. First published July 16, 2010 doi:10.1152/ajplung.00111.2010.-TNF␣, a proinflammatory cytokine known to be involved in the pathogenesis of allergic asthma, has been shown to induce hyperalgesia in somatic tissue via a sensitizing effect on dorsal root ganglion neurons expressing transient receptor potential vanilloid type 1 receptor (TRPV1). Because TRPV1-expressing pulmonary sensory neurons play an important role in regulating airway function, this study was carried out to determine whether TNF␣ alters the sensitivity of these neurons to chemical activators. Responses of isolated nodose and jugular ganglion neurons innervating the rat lungs were determined by measuring the transient increase in intracellular Ca 2ϩ concentration ([Ca 2ϩ ]i). Our results showed the following. 1) A pretreatment with TNF␣ (50 ng/ml) for ϳ24 h increased significantly the peak ⌬[Ca 2ϩ ]i evoked by capsaicin (Cap) in these neurons. A pretreatment with the same concentration of TNF␣ for a longer duration (ϳ48 h) did not further increase the response, but pretreatment for a shorter duration (1 h) or with a lower concentration (25 ng/ml, 24 h) failed to enhance the Cap sensitivity.2) The same TNF␣ pretreatment also induced similar but less pronounced and less uniform increases in the responses to acid (pH 6.5-5.5), 2-aminoethoxydiphenyl borate (2-APB), a common activator of TRPV1, V2, and V3 channels, and allyl isothiocyanate (AITC), a selective activator of TRPA1 channel. 3) In sharp contrast, the responses to ATP, ACh, and KCl were not affected by TNF␣. 4) The TNF␣-induced hypersensitivity to Cap was not prevented by pretreatment with indomethacin (30 M). 5) The immunoreactivity to both TNF receptor types 1 and 2 were detected in rat vagal pulmonary sensory neurons. In conclusion, prolonged treatment with TNF␣ induces a pronounced potentiating effect on the responses of isolated pulmonary sensory neurons to TRPV1 activators. This action of TNF␣ may contribute in part to the airway hyperresponsiveness induced by this cytokine. intracellular calcium; pulmonary afferent; transient receptor potential vanilloid type 1; airway inflammation; asthma AN IMPORTANT ROLE OF TNF␣ in the pathogenesis of allergic asthma has been extensively documented (6,9,28,47). TNF␣ can be released from a variety of cell types in the airways, particularly macrophages, monocytes, and mast cells. TNF␣, either newly synthesized or preformed and stored constitutively within the mast cell granules, is coreleased with other potent mediators such as histamine and tryptase via the IgE-dependent mechanism (9, 21, 47). TNF␣ was detected in bronchoalveolar lavage fluid, sputum, exhaled breath condensate, and serum of asthmatic patients during asthmatic attack or following antigen inhalation challenge (12,33,39). Furthermore, it has been shown that TNF␣ is releas...

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confidence: 99%

mentioning

confidence: 99%

Calcium transient evoked by TRPV1 activators is enhanced by tumor necrosis factor-α in rat pulmonary sensory neurons

Gu²,

Lin³

et al. 2010

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…In recent years, there has been increasing interest in the role of lung inflammation in the development and progression of a variety of lung diseases as diverse as pulmonary fibrosis [1], cystic fibrosis [2], idiopathic bronchiectasis [3], chronic obstructive pulmonary disease (COPD) [4] and asthma [5]. These studies have been facilitated by the widespread availability of commercial assays to measure the inflammatory mediators of interest.…”

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Validation of assays for inflammatory mediators in sputum

Stockley¹,

Bayley²

2000

Eur Respir J

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Commercially available immune assays are being used with increasing frequency in the study of lung inflammation. However, their performance in complex biological fluids is rarely assessed. The authors wished to assess their reliability to determine whether the results obtained in sputum samples can be easily interpreted.The reproducibility of several such assays was therefore determined together with their ability to recover known amounts of pure reagent. Sputum sol phase was obtained from several patients with chronic lung disease and used together with the reagents in a series of "spiking" and dilutional experiments.Results confirmed that the enzyme assay for myeloperoxidase and the immune assays for interleukin-8, leukotriene B 4 and secretory leukoproteinase inhibitor were all reproducible (intra-assay coefficient of variation 3.8±7.7%). Furthermore, each of these assays gave >85% recovery of a "spike" with pure reagent. However, the immune assay for myeloperoxidase (although reproducible) gave poor recovery and was dependent on the degree of sample dilution and elastase content.These studies confirm that the reliabilities of fluid phase measurements should be assessed before being widely applied to complex biological samples. Eur Respir J 2000; 15: 778±781.

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“…29) In the event, stimulation of HPBMC with lipopolysaccharide leads to production of TNF-a, which was nicely suppressed by our new rolipram analogues (Fig. 2).…”

Section: Resultsmentioning

confidence: 68%

Synthesis and Structure-Activity Relationship of N-Arylrolipram Derivatives as Inhibitors of PDE4 Isozymes.

Keller

Bray‐French

Demnitz

et al. 2001

CHEMICAL & PHARMACEUTICAL BULLETIN

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Late response to allergen is associated with increased concentrations of tumor necrosis factor-α and IL-5 in induced sputum

Cited by 95 publications

References 18 publications

Calcium transient evoked by TRPV1 activators is enhanced by tumor necrosis factor-α in rat pulmonary sensory neurons

Calcium transient evoked by TRPV1 activators is enhanced by tumor necrosis factor-α in rat pulmonary sensory neurons

Validation of assays for inflammatory mediators in sputum

Synthesis and Structure-Activity Relationship of N-Arylrolipram Derivatives as Inhibitors of PDE4 Isozymes.

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