Last performance with VIAGRA®: post-mortem identification of sildenafil and its metabolites in biological specimens including hair sample

Dumestre-Toulet, Véronique; Cirimele, Vincent; Gromb, S.; Belooussoff, T.; Lavault, D.; Ludes, Bertrand; Kintz, Pascal

doi:10.1016/s0379-0738(02)00012-9

Cited by 37 publications

(23 citation statements)

References 9 publications

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“…Several high-performance liquid chromatographic (HPLC) methods have been reported for the determination of sildenafil and/or N-desmethyl sildenafil in biological samples. Gas chromatography-mass spectrometry (GC/MS) [2], micellar electrokinetic chromatography [3], liquid chromatographymass spectrometry (LC/MS) [4,5] as well as liquid chromatography-tandem mass spectrometry (LC/MS/MS) [6][7][8] methods have been reported. Liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry (LC/MS/MS) are expensive and thus unavailable in many laboratories.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sildenafil and N-desmethyl sildenafil in human plasma by high-performance liquid chromatography method using electrochemical detection with application to a pharmacokinetic study

Al-Ghazawi

Tutunji

AbuRuz

2007

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sildenafil and N-desmethyl sildenafil in human plasma by high-performance liquid chromatography method using electrochemical detection with application to a pharmacokinetic study

Al-Ghazawi

Tutunji

AbuRuz

2007

Journal of Pharmaceutical and Biomedical Analysis

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“…10,14,15 Sildenafil citrate is a novel, prototype drug for erectile dysfunction, which inhibits PDE-5 selectively. 16 Inhibition of PDE-5 is shown to enhance penile erection by preventing cGMP degradation. PDE inhibitors have been demonstrated to achieve improvement in erectile dysfunction.…”

mentioning

confidence: 99%

Inhibitory Effects of Sildenafil Citrate on the Tonus of Isolated Dog Internal Anal Sphincter

Aygen

Camcı

Durmus

et al. 2005

Diseases of the Colon &Amp; Rectum

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This experimental in vitro study showed that sildenafil citrate relaxes acetylcholine stimulated contractions of isolated dog internal anal sphincter. This may be of importance for raising the possibility that sildenafil cit-rate may have future potential in the treatment of chronic anal fissure. Further studies are needed for a conclusive decision on possible usefulness of sildenafil citrate in patients with chronic anal fissure.

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“…The lower limits of detection under the scan mode were 0.05 ng for vardenaˆl (as HCl･ 3H 2 O salt), 0.1 ng for both sildenaˆl (as citrate salt) and hydroxyhomosildenaˆl and 0.25 ng for tadalaˆl in terms of on-column amount (S/N＞ 5). Under the selected ion monitoring of their protonated molecules, the sensitivities were enhanced about 10 times, which was comparable to those required to analyze biological samples 12,20,21) . Quantitative analysis of the compounds was carried out using HPLC (UV 230 nm).…”

Section: Resultsmentioning

confidence: 95%

Chemical Profile of Sildenafil and Related Compounds

Inoue

Kanamori

Kuwayama

et al. 2008

Jpn. J. Forensic. Sci. Tech.

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Sildenaˆland related compounds, vardenaˆl, tadalaˆl and hydroxyhomosildenaˆl, in dietary supplements were characterized using thin layer chromatography (TLC), infrared (IR) spectroscopy, gas chromatography/mass spectrometry (GC/MS), high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). TLC with DragendorŠ reagent gave a lower detection limit than 1 mg for all 4 compounds. IR spectra of the compounds were so characteristic as to discriminate one from another easily. After TMS derivatization, all the peaks of these compounds appeared and well-separated on the GC/MS chromatograms at a high column temperature (320°C), although hydroxyhomosildenaˆl was not detected without derivatization. In HPLC and LC/ MS analyses, an ODS column with a mobile phase composed of a mixture of 20 mM ammonium acetate (pH 5.0) and methanol (4060) allowed separation of the 4 compounds. Simple electrospray-ionization mass spectra with only protonated molecules were obtained except for tadalaˆl, which was cleaved easier than the other compounds, so cone voltage should be lower for tadalaˆl. Quantitative analysis of the 4 compounds was carried out using HPLC (UV at 230 nm). The lower limit of detection for each compound was 50 ng/ml at a 10-ml injection volume (0.5 ng on the column, S/N＞3). A calibration curve was linear in the range of 0.01 1.0 mg/ml for each compound (r 2 ＞0.997). Our methods were applied to casework samples (conˆscated tablets), and disclosed that those tablets contained varying amounts of sildenaˆl and/or tadalaˆl and were counterfeit products.

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Last performance with VIAGRA®: post-mortem identification of sildenafil and its metabolites in biological specimens including hair sample

Cited by 37 publications

References 9 publications

Simultaneous determination of sildenafil and N-desmethyl sildenafil in human plasma by high-performance liquid chromatography method using electrochemical detection with application to a pharmacokinetic study

Simultaneous determination of sildenafil and N-desmethyl sildenafil in human plasma by high-performance liquid chromatography method using electrochemical detection with application to a pharmacokinetic study

Inhibitory Effects of Sildenafil Citrate on the Tonus of Isolated Dog Internal Anal Sphincter

Chemical Profile of Sildenafil and Related Compounds

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