Lasers, dermal fibroblasts and psoriasis

Saïag, P.; Rowland‐Payne, Christopher; Dubertret, Louis; Touraine, R

doi:10.1111/j.1365-2133.1986.tb06662.x

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…7 Reproducibility of this finding depends on the anatomical location of the fibroblast (upper versus deeper dermis), the type of fibroblast culture (fibroblasts incorporated in collagen lattice versus monoculture) and epidermal cell culture (biopsy versus monolayer) used in the fibroblast-keratinocyte co-culture model. 5,8,9 All together, studies suggest the involvement of fibroblastderived cytokines in the pathogenesis of psoriasis. For instance, human dermal fibroblasts are able to produce interleukin(IL-)1, IL-6 and IL-8, [10][11][12] which are not only actively involved in inflammation but are also mitogenic for epidermal cells.…”

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Expression of cytokines and their receptors by psoriatic fibroblasts I. Altered IL-6 synthesis

et al. 1996

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Interleukin(IL-)6 is overproduced in psoriatic lesions. We investigated the contribution of dermal fibroblasts to the local IL-6 production. Fibroblasts (passageno. 2 to 6) derived from lesional psoriatic (PP) and normal human (NN) skin were used to analyse the secretion of IL-6, and the related cytokines IL-1, IL-8 and TNF-alpha, and the expression of their corresponding mRNA by bioassay, ELISA and Northern hybridization, respectively. PP fibroblasts cultured under serum-free conditions produced increased amounts of bioactive IL-6 when compared to NN fibroblasts. Differences were partially restored in the presence of growth factors or serum. The serum-induced IL-6 production reached a maximum within 24 h after seeding and remained unchanged in PP fibroblasts, whereas comparable amounts of IL-6 were produced only 6 days later in NN fibroblasts. There was a clear expression of IL-6 mRNA in both types of fibroblasts under serum-free conditions. Unexpectedly, fetal calf serum, inactivated fetal calf serum as well as human serum completely inhibited the expression of IL-6 mRNA in all the PP fibroblast cultures investigated. NN fibroblasts were clearly less sensitive to this inhibiting effect of serum. Furthermore, medium supplemented with serum-free component or calcium also repressed IL-6 mRNA expression in PP fibroblasts in contrast to NN fibroblasts. Cycloheximide fully restored the repressing effect of serum indicating that serum induced a labile repressor protein. PP and NN fibroblasts produced negligible amounts of IL-1 and TNF-alpha, but the production of IL-8, however, was comparable to that of IL-6. Our results show a differently regulated IL-6 synthesis in PP fibroblasts in vitro, suggesting an active contribution of dermal fibroblasts to the local IL-6 production in psoriasis.

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Expression of cytokines and their receptors by psoriatic fibroblasts I. Altered IL-6 synthesis

et al. 1996

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“… 12 Saiag et al believe that superficial dermal fibroblasts are responsible in psoriasis. 13 This laser has a potential for penetrating deep in the dermis and affecting papillary and reticular dermal fibroblasts. A biomodulatory effect in these cells is possible, either stimulating or inhibiting cell activity.…”

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confidence: 99%