Laser-ultraviolet-A-induced ultraweak photon emission in mammalian cells

Abstract

Photobiological research in the last 30 yr has shown the existence of ultraweak photon emission in biological tissue, which can be detected with sophisticated photomultiplier systems. Although the emission of this ultraweak radiation, often termed biophotons, is extremely low in mammalian cells, it can be efficiently increased by ultraviolet light. Most recently it was shown that UV-A (330 to 380 nm) releases such very weak cell radiation in differentiated human skin fibroblasts. Based on these findings, a new and powerful tool in the form of UV-A-laser-induced biophotonic emission of cultured cells was developed with the intention to detect biophysical changes between carcinogenic and normal cells. With suspension densities ranging from 1 to 8 x 10(6) cells/mL, it was evident that an increase of the UV-A-laser-light induced photon emission intensity could be observed in normal as well as melanoma cells. Using this new detection procedure of ultraweak light emission, photons in cell suspensions as low as 100 microL could be determined, which is a factor of 100 lower compared to previous procedures. Moreover, the detection procedure has been further refined by turning off the photomultiplier system electronically during irradiation leading to the first measurements of induced light emission in the cells after less than 10 micros instead of 150 ms, as reported in previous procedures. This improvement leads to measurements of light bursts up 10(7) photons/s instead of several hundred as found with classical designs. Overall, we find decreasing induction ratings between normal and melanoma cells as well as cancer-prone and melanoma cells. Therefore, it turns out that this highly sensitive and noninvasive device enables us to detect high levels of ultraweak photon emission following UV-A-laser-induced light stimulation within the cells, which enables future development of new biophysical strategies in cell research.

Biophotons: Ultraweak Light Impulses Regulate Life Processes in Aging

“…Furthermore, in the case of cancer cells, the cellular toxins accumulated during the years, and often leading to deposits of cellular debris in the tissues (e.g. arteriosclerosis in blood vessels), induce the increased emission of biophotons [27,28].…”

“…Furthermore, in the case of cancer cells, the cellular toxins accumulated during the years, and often leading to deposits of cellular debris in the tissues (e.g. arteriosclerosis in blood vessels), induce the increased emission of biophotons [27,28]. [28,29] developed a new highly sensitive analytic photomultiplier system allowing for the first time to measure the spectrum of ultraweak photon emission in individual human cells.…”

“…arteriosclerosis in blood vessels), induce the increased emission of biophotons [27,28]. [28,29] developed a new highly sensitive analytic photomultiplier system allowing for the first time to measure the spectrum of ultraweak photon emission in individual human cells. These researchers achieved a crucial improvement of the technique of ultra-weak light measurement [29].…”

“…Furthermore, in the case of cancer cells, the cellular toxins accumulated during the years, and often leading to deposits of cellular debris in the tissues (e.g. arteriosclerosis in blood vessels), induce the increased emission of biophotons [27,28].Karl-Heinrich Müller who died in 2007, is the founder and initiator of the Island of Museum and Art "Hombroich", a paradisiacal garden and dreamland of wonderful beauty. Fritz-Albert Popp and Karl-Heinrich Müller established in the mid-nineties a Center of Biophotonics at a former rocket station close to this island of art (in Neuss nearby Düsseldorf, Germany).…”

Subliminal Afterimages Via Ocular Delayed Luminescence: Transsaccade Stability of the Visual Perception and Color Illusion

“…The DL intensity is considerably lower than the known fluorescence or phosphorescence. Although externally measured intensity of spontaneous biophoton emission from mammalian is extremely low intensity (from a few to up to some hundred photons/(cm 2 x s), it can be increased via external illumination (Kim et al, 2005;Niggli et al, 2005). The decay time of delayed luminescence is dependent on the physiological conditions of the samples and kinds of the tissues as well as intensity, duration, and spectral distribution of illumination (Niggli et al, 2005).…”