Classification of Chinese herbs based on the cluster analysis of delayed luminescence

Cryopreservation, the most common method of preserving stem cells, requires post-processing because it produces trauma to the cells. Post-thawing trauma typically induces cell death, elevates reactive oxygen species (ROS) concentration, and lowers mitochondrial membrane potential (MMP). Although this trauma has been solved using antioxidants, we attempted to use photobiomodulation (PBM) instead of chemical treatment. We used a 950-nm near-infrared LED to create a PBM device and chose a pulsed-wave mode of 30 Hz and a 30% duty cycle. Near-infrared radiation (NIR) at 950 nm was effective in reducing cell death caused by hydrogen peroxide induced-oxidative stress. Cryodamage also leads to apoptosis of cells, which can be avoided by irradiation at 950 nm NIR. Irradiation as post-processing for cryopreservation had an antioxidant effect that reduced both cellular and mitochondrial ROS. It also increased mitochondrial mass and activated mitochondrial activity, resulting in increased MMP, ATP generation, and increased cytochrome c oxidase activity. In addition, NIR increased alkaline phosphatase (ALP) activity, a biomarker of differentiation. As a result, we identified that 950 nm NIR PBM solves cryodamage in human stem cells from the apical papilla, indicating its potential as an alternative to antioxidants for treatment of post-thawing trauma, and further estimated its mechanism.

show abstract

“…Delayed luminescence follows Eq. ( 1 ) which is the hyperbolic function 51 . The curves of NIR intensity were fitted in Matlab.…”

Section: Methodsmentioning

confidence: 99%

Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla

Choi

Baik

Jeong

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The DL decay curve for each sample, measured over a 60-s period, was fitted to the following hyperbolic function [ 22 ]: where I 0 is the initial intensity of the DL curve, Beta is an index factor associated with the rate of DL decay, and Tau and T represents the DL characteristics and decay time, respectively [ 22 ]. The R package nnet (version 3.2.2) was used to perform the DL curve-fitting [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Application of delayed luminescence method on measuring of the processing of Chinese herbal materials

Sun

Chang

Wijk³

et al. 2018

Chin Med

Self Cite

View full text Add to dashboard Cite

BackgroundBased on the principle of tradition Chinese medicine, the processing refers to various techniques that alter the overall properties of herbal materials to meet the requirements of therapeutic applications. However, the standards of quality control and scientific standard operation protocol for processing manufacturing are largely unknown and there is a huge demand for the development of scientific tools for evaluating the quality during and after the processing. The key challenge in evidence-based medicine is to characterize the processing of herbal materials from system-based perspective.MethodsDelayed luminescence (DL) as a rapid, direct, systemic tool was used to characterize the properties of raw and processed materials of Rehmanniae radix and Ginseng radix et rhizome. Hyperbolic function was used to extract four parameters from DL curves of herbal materials. Statistical tools, including one-way analysis of variance and principal component analysis, were used to differentiate raw and processed herbal materials.ResultsOur results showed DL properties were able to reliably identify raw and processed materials of Rehmanniae radix and Ginseng radix et rhizoma, respectively. In addition, the results indicated that after four cycles of processing for Rehmanniae radix, there was no much significant change in DL parameters which resembles the results obtained from chemical analyses (after five cycles) using 1HNMR and gas chromatography–mass spectrometry in previous studies.ConclusionDL may serve as a fast, robust and sensitive tool for evaluating processing on herbs and may be used as part of a comprehensive platform for assessing the quality of herbal materials.Electronic supplementary materialThe online version of this article (10.1186/s13020-018-0202-0) contains supplementary material, which is available to authorized users.

show abstract

“…This hyperbolic function is a general formula for fitting the DL decay curve of samples. The four parameters (I0, Beta, Tau and T) obtained by this hyperbolic function can well express the characteristics of the DL decay curve [24,28]. I0 is the initial intensity of the DL curve, Beta is an index factor associated with the rate of DL decay, and Tau and T represent the DL characteristics and decay time, respectively.…”

Section: Statistical Analysis Of DL Propertiesmentioning

confidence: 99%

A comparative study of aged and contemporary Chinese herbal materials by using delayed luminescence technique

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: Investigation of aged Chinese herbal materials will help us to understand their use and sources in ancient time and broaden the historical perspective of Chinese material medica. To reach this aim, the basic understanding of aged herbal materials, including physical and chemical characters, is of great importance. Delayed luminescence (DL) technique was developed as a rapid, direct, systemic, objective and sample loss-free tool to characterize the properties of Chinese herbal materials. In this study, we measured DL values in aged Chinese herbal materials that were transported from Asia to Europe during the 20th century and stored in Naturalis Biodiversity Center and the Utrecht University museum, and compared these with modern material of the same species. Methods: A hyperbolic function was used to extract four properties from the DL curves of Chinese herbal material from 1900, the 1950s and recently harvested products. Statistical tools, including the Student's t test, One-way analysis of variance and Principal Component Analysis, were used to differentiate the DL properties of aged and contemporary collections of Glycyrrhiza spp. Curcuma aromatica Salisb., Zingiber officinale Roscoe, Alpinia officinarum Hance and Acorus calamus L. Results: Our results showed that DL properties were significantly different between historical and contemporary Chinese herbal materials. Changes in DL values were species-dependent: the effects of storage time of DL properties were specific for each species. These outcomes help us not only in the identification of historical Chinese medicine products but also provides valuable data of the effect of storage time on herbal materials. Conclusion: The simple, direct, rapid, and inexpensive measurements offered by DL provide a novel tool to assess the taxonomic identity of Chinese and other herbal materials and assess the differences in chemical properties with increasing storage time. Our results contribute to the further development of novel digital tools for the quality control of herbal materials.

show abstract

Classification of Chinese herbs based on the cluster analysis of delayed luminescence

Cited by 9 publications

References 35 publications

Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla

Photobiomodulation as an antioxidant substitute in post-thawing trauma of human stem cells from the apical papilla

Application of delayed luminescence method on measuring of the processing of Chinese herbal materials

A comparative study of aged and contemporary Chinese herbal materials by using delayed luminescence technique

Contact Info

Product

Resources

About