Laser spectroscopy in analytical chemistry:

Gooijer, C.; Mank, A.

doi:10.1016/s0003-2670(99)00656-x

Cited by 30 publications

(16 citation statements)

References 45 publications

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“…Among the detection modes commonly used in CE, the fluorescence detection as a highly sensitive and selective technology, are frequently used in routine biotechnological analysis. Lasers and discharge light sources are widely used as excitation sources in fluorescence detection [1,2]. The common limitations of these light sources are the bulky size, high power consumption and limited lifetime, which is one of the most expensive parts in CE equipment.…”

Section: Introductionmentioning

confidence: 99%

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Yang

Tan

Guan

2005

Talanta

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Section: Introductionmentioning

confidence: 99%

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Yang

Tan

Guan

2005

Talanta

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“…The stability of a HeCd laser is typically only 1%, 29 whereas that for diode lasers is 0.01%. 22 With our lasers and CE instrument, we observed a 1.4% fluctuation in fluorescence excited using the HeCd laser, and a 0.09% fluctuation with the violet diode laser. 30 Thus while the fluorescence signal is higher for the HeCd laser, so is the background noise.…”

Section: Hqs Detection Of Metalsmentioning

confidence: 69%

“…22 These properties suggest that diode lasers are ideally suited for LIF detection. In October of 1999, Nichia Corporation (Japan) launched the commercial sale of their InGaN-based violet laser diode.…”

mentioning

confidence: 99%

Violet Diode Laser for Metal Ion Determination by Capillary Electrophoresis-Laser Induced Fluorescence

Vos

Lucy

2001

ANAL. SCI.

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Since the work of Jorgenson and Lukacs in the early 1980s, 1,2 capillary electrophoresis (CE) has experienced rapid growth and been applied to a wide array of analytes. The dominant area of application of CE has been for biomolecules. However, there has been a steady and increasing interest in using CE for the determination of metal ions. 3The attractive features of CE for metal ion determination are the same as those for other separations: its exceptional separation efficiency; its rapid separations; and its low mass detection limits. However, as with other types of analytes, the primary limitation of CE is its poor concentration detection limits. Most (85%) publications of CE for metal ions have used absorbance detection. 3 Unfortunately, few hydrated metal ions absorb significantly above 185 nm. Greater sensitivity is achieved by complexing the metal ions with a metallochromic dye. Examples include PAR for transition metals, 4 Arsenazo I for alkaline earth metals 5 and Arsenate III for lanthanides and uranium. 6,7 Alternatively, up to 26 metal cations have been separated with parts-per-billion detection limits using indirect absorbance detection. [8][9][10][11] Regardless, the most sensitive detection scheme in CE is laser induced fluorescence (LIF). 12-14 CE-LIF determinations of metals typically use the 8-hydroxyquinoline-5-sulfonic acid (HQS) fluorescent ligand shown in Fig. 1 . Optimum excitation of the metal-HQS is at 393 nm with maximum emission in the 500 -520 nm range. 17 Fluorescence excitation of metal-HQS complexes separated by CE has been performed using the 325 nm HeCd laser line, 15,18,19 the 351.1 -364.8 nm lines of an argon ion laser 20 and with a broad band mercury-xenon lamp. 16 However, none of these excitation sources is ideal. They all are expensive and have limited lifetimes (∼5000 h). They are also relatively bulky, making them unattractive for miniaturized instruments. 19 Semiconductor or diode lasers are promising alternatives to conventional lasers. Diode lasers are cheap, require no maintenance, and have lifetimes approaching 50000 h. 21 These lasers are also extremely compact and can run off battery power.Further, the output stability of diode lasers is significantly better than that of conventional lasers (0.01% vs. 1% noise). 22 These properties suggest that diode lasers are ideally suited for LIF detection. In October of 1999, Nichia Corporation (Japan) launched the commercial sale of their InGaN-based violet laser diode.23-27 * The diode operates at 405 nm with a power output of 5 mW and has an estimated lifetime of 2000 to 5000 h. Such short wavelength semiconductor lasers have long been sought for applications such as full-color electroluminescent displays, laser printers, undersea optical communications and optical data storage. For instance, a violet diode laser would quadruple the data capacity of current DVD players.In addition to its applications in electronics and telecommunications, the violet diode laser offers tremendous potential for analytical chemistry. Recently, ...

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“…Since fluorescence intensity is dependent on both pathlength and incident power, the use of lasers as an excitation source greatly improves the detection sensitivity. Thus, one way to increase the sensitivity of CE has been LIFD detection [23,24].…”

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

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Laser spectroscopy in analytical chemistry:

Cited by 30 publications

References 45 publications

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Violet Diode Laser for Metal Ion Determination by Capillary Electrophoresis-Laser Induced Fluorescence

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

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