Laser speckle dynamic for monitoring fruits maturation

Nassif, Rana; Pellen, Fabrice; Magné, Christian; Jeune, Bernard; Brun, Guy Le; Abboud, Maher

doi:10.1117/12.975782

Cited by 2 publications

(1 citation statement)

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“…The grain size is one important parameter, generally is used in speckle images to characterize the surface roughness and smoothness. It is defined as the width of the autocorrelation of tissue images [23]. In our work, we use it to determinate if there are significant changes in retinal and lens tissues that normally cannot be detected in microscopy images.…”

Section: Introductionmentioning

confidence: 99%

Alteration of lens and retina textures from mice embryos with folic acid deficiency: image processing analysis

Sijilmassi

López-Alonso²,

Asensio

et al. 2018

Graefes Arch Clin Exp Ophthalmol

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Purpose: Folic Acid (FA) is an essential vitamin for embryonic development. It plays particularly a critical role in RNA, DNA, and protein synthesis. On the other hand, the collagen IV and laminin-1 are important proteins during embryonic development. This study was done to find if FA-deficiency at a short and a long-term in mothers could alter the tissue texture of retina and lens of the progeny. Methods: Collagen IV and laminin-1 were localized by immunohistochemistry in the lens and retina of the FA-deficient embryos. To carry out the image processing, texture segmentation was performed through canny edge detection and Fourier transform (FT). We defined a parameter, the grain size, to describe the texture of the lens and retina. A bootstrap method to estimate the distribution and confidence intervals of the mean, standard deviation, skewness and kurtosis of the grain size has been developed. Results: Analysis through image processing using Matlab showed changes in the grain size between control and FA deficient groups in both studied molecules. Measures of texture based on FT exhibited changes in the directionality and arrangements of type IV collagen and laminin-1. Conclusions: Changes introduced by FA deficiency were visible in the short-term (two weeks) and evident in the long term (eight weeks) in both grain size and orientation of fibre structures in the tissues analysed (lens and retina). This is the first work devoted to study the effect of FA deficit in the texture of eye tissues using image processing techniques.

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Section: Introductionmentioning

confidence: 99%