Laser scanning confocal microscopy of cervical tissue before and after application of acetic acid

Drezek, Rebekah A.; Collier, Tom; Brookner, Carrie; Malpica, Anaís; Lotan, Reuben; Richards‐Kortum, Rebecca; Follen, Michele

doi:10.1067/mob.2000.104844

Cited by 116 publications

(90 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The effects of acetic acid, commonly used to aid in the detection of precancerous changes in the cervix, have been well-established from reflectance confocal microscopy [16,17]. In particular, it has been shown that acetic acid increases scattering from cell nuclei due to nuclear coagulation.…”

Section: Animal Preparationsmentioning

confidence: 99%

Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast

Leahy

Radhakrishnan

Srinivasan

2013

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:We present volumetric imaging and computational techniques to quantify neuronal and myelin architecture with intrinsic scattering contrast. Using spectral / Fourier domain Optical Coherence Microscopy (OCM) and software focus-tracking we validate imaging of neuronal cytoarchitecture and demonstrate quantification in the rodent cortex in vivo. Additionally, by ex vivo imaging in conjunction with optical clearing techniques, we demonstrate that intrinsic scattering contrast is preserved in the brain, even after sacrifice and fixation. We volumetrically image cytoarchitecture and myeloarchitecture ex vivo across the entire depth of the rodent cortex. Cellular-level imaging up to the working distance of our objective (~3 mm) is demonstrated ex vivo. Architectonic features show the expected laminar characteristics; moreover, changes in contrast after the application of acetic acid suggest that entire neuronal cell bodies are responsible for the "negative contrast" present in the images. Clearing and imaging techniques that preserve tissue architectural integrity have the potential to enable noninvasive studies of the brain during development, disease, and remodeling, even in samples where exogenous labeling is impractical.

show abstract

Section: Animal Preparationsmentioning

confidence: 99%

Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast

Leahy

Radhakrishnan

Srinivasan

2013

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…Pilot cervical tissue optical spectroscopy studies have been reviewed by Thekkek et al in 2008 [13] and the review shows that this technology can achieve sensitivities and specificities in the range of 83 -92% and 80 -90%, respectively, thus having the potential to address the limited specificity of VIA or VIAM. The primary sources of intrinsic absorption and scattering contrast in cervical neoplasia are increased absorption associated with neovascularization in the stroma [15][16][17], increased epithelial scattering with cell proliferation, and decreased stromal scattering with collagen degradation [18][19][20] (dominant source of multiply backscattered light in the visible wavelengths [21]). Using immunohistochemical stains for vascular endothelial cells, Abulafia et al [22] and Dellas et al [15] have demonstrated that neovascularization, quantified through microvessel density, is positively correlated with increasing cervical dysplastic grades.…”

Section: Introductionmentioning

confidence: 99%

Towards a field-compatible optical Spectroscopic device for cervical cancer screening in resource-limited settings: effects of calibration and pressure

Chang¹,

Merisier²,

Yu³

et al. 2011

Opt. Express

View full text Add to dashboard Cite

Abstract:Quantitative optical spectroscopy has the potential to provide an effective low cost, and portable solution for cervical pre-cancer screening in resource-limited communities. However, clinical studies to validate the use of this technology in resource-limited settings require low power consumption and good quality control that is minimally influenced by the operator or variable environmental conditions in the field. The goal of this study was to evaluate the effects of two sources of potential error: calibration and pressure on the extraction of absorption and scattering properties of normal cervical tissues in a resource-limited setting in Leogane, Haiti. Our results show that self-calibrated measurements improved scattering measurements through real-time correction of system drift, in addition to minimizing the time required for post-calibration. Variations in pressure (tested without the potential confounding effects of calibration error) caused local changes in vasculature and scatterer density that significantly impacted the tissue absorption and scattering properties Future spectroscopic systems intended for clinical use, particularly where operator training is not viable and environmental conditions unpredictable, should incorporate a real-time self-calibration channel and collect diffuse reflectance spectra at a consistent pressure to maximize data integrity. ©2011 Optical Society of America

show abstract

“…Because of the characteristic in the absorption spectrum of hemoglobin, optical absorption around the wavelengths of 420 nm , 542 nm and 577 nm is increased remarkably. The application of acetic acid elevates the mean scattering coefficient of precancerous tissue approximately three times that of normal epithelium, making abnormal tissue appear whiter than normal, which is a consequence of the increased nucleus density and size, as well as the potential change of the chromatin characters [8,9]. The decreased stromal scattering, which is associated with a degradation of collagen fibers, also can be observed in precancerous cervical tissue [10].…”

Section: Introductionmentioning

confidence: 81%

Detection of cervical cancer based on photoacoustic imaging—the in-vitro results

Peng

Wang

et al. 2014

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:In current clinical practice, the diagnosis of cervical cancer (CC) is mainly through the cervical screening followed by a necessary biopsy, but this method is labor consuming and expensive, and can only detect superficial lesions around the external cervical orifice. In contrast, photoacoustic imaging (PAI) is sensitive to the abnormal angiogenesis deep in the biological tissue, and may be capable for the intact scanning both around the external orifice and in cervical canal. In this paper, we for the first time put forward the photoacoustic diagnosis of CC. A total of 30 invitro experiments were carried out in this study, and the obtained depth maximum amplitude projection (DMAP) images were analyzed to evaluate the extent of the angiogenesis for different clinical stages of CC. Stronger absorption from the cervical lesions is observed relative to that of normal tissue. Paired t-test indicates that the difference in mean optical absorption (MOA) between normal tissue and cervical lesion has statistical significance with a confidential coefficient of 0.05. Statistical results also show that the MOAs of the cervical lesions are closely related to the severity of CC. These results imply that PAI may have great utility in the clinical diagnosis of CC. Phys. 39(11), 6895-6899 (2012).

show abstract

Laser scanning confocal microscopy of cervical tissue before and after application of acetic acid

Cited by 116 publications

References 17 publications

Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast

Volumetric imaging and quantification of cytoarchitecture and myeloarchitecture with intrinsic scattering contrast

Towards a field-compatible optical Spectroscopic device for cervical cancer screening in resource-limited settings: effects of calibration and pressure

Detection of cervical cancer based on photoacoustic imaging—the in-vitro results

Contact Info

Product

Resources

About