Laser nanostructuring 3-D bioconstruction based on carbon nanotubes in a water matrix of albumin

Gerasimenko, Alexander Yu.; Ичкитидзе, Л. П.; Podgaetsky, Vitaly M.; Savelyev, Mikhail S.; Селищев, С. В.

doi:10.1117/12.2219929

Cited by 10 publications

(6 citation statements)

References 10 publications

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“…The first step was the preparation of an aqueous colloidal solution of SWCNTs (dispersing medium). 22 To do this, SWCNTs with the concentration of 0.1 g∕L were added to pure water to research the cells. The obtained solution was subjected to the powerful ultrasonic probe homogenizer (time, 30 to 40 min; power, 40 W) in order to eliminate discontinuities associated with the conglomeration of SWCNTs in bundles.…”

Section: Methodsmentioning

confidence: 99%

“…Besides, defective areas will heat up most noticeably under heating tubes by a laser beam, as the thermal conductivity is markedly reduced in these areas. 23 We considered two tubes with the chiral indices (22,22) and (24,24) as their diameters are the closest to the data yielded by the experiment-1.4 and 1.6 nm, respectively. We simulated the most common structural defects: single vacancy (1V) defect, double vacancy (2V) defect, and Stone-Wales (SW) defect.…”

Section: Creation Of the Composite Biostructuresmentioning

confidence: 99%

“…shows the results of the numerical experiments.Figure 3(a)shows "the splice" of an open end of the nanotube(22,22) with the defective area of the nanotube(24,24). The defective area contains two 2V defects and one SW defect.…”

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confidence: 99%

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Laser structuring of carbon nanotubes in the albumin matrix for the creation of composite biostructures

et al. 2017

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This paper presents the composite biostructures created by laser structuring of the single-walled carbon nanotubes (SWCNTs) in an albumin matrix. Under the exposure of femtosecond laser radiation, the heating of the albumin aqueous solution causes liquid water to evaporate. As a result, we obtained a solid-state composite in the bulk or film form. Using the molecular dynamic method, we showed the formation of a framework from SWCNTs by the example of splicing of the open end of one nanotube with the defect region of another nanotube under the action of the laser heating. Laser heating of SWCNTs up to a temperature of 80°C to 100°C causes the C ? C bond formation. Raman spectra measured for the composite biostructures allowed us to describe the binding of oxygen atoms of amino acid residues of the albumin with the carbon atoms of the SWCNTs. It is found that the interaction energy of the nanotube atoms and albumin atoms amounts up to 580 ?? kJ / mol . We used atomic force microscopy to investigate the surface of the composite biostructures. The pore size is in the range of 30 to 120 nm. It is proved that the proliferation of the fibroblasts occurred on the surface of the composite biostructures during 72 h of incubation.

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Section: Methodsmentioning

confidence: 99%

Section: Creation Of the Composite Biostructuresmentioning

confidence: 99%

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Laser structuring of carbon nanotubes in the albumin matrix for the creation of composite biostructures

et al. 2017

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“…MWCNTs were synthesized by a low-temperature thermo-catalytic method on a Ni catalyst and consisted of a black powder. The external diameter of MWCNTs was 40-120 nm, length was 1-10 lm, and internal diameter was 10-30 nm [3]. As the technology for coatings making requires thermal heating of the dispersion, bovine serum albumin (BSA) was selected as a protein with maximal resistance to temperatures.…”

Section: Methodsmentioning

confidence: 99%

Laser Technology of Designing Nanocomposite Implants of the Knee Ligaments

Gerasimenko

Kurilova

Мезенцева

et al. 2017

Proceedings of the Scientific-Practical Conference "Research and Development - 2016"

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We describe a laser method for constructing a biocompatible implant of the knee ligaments based on synthetic-braided fiber structure of polyethylene terephthalate (PET) coated with a nanocomposite coating. A coating based on albumin aqueous dispersion of carbon nanotubes (CNTs) was applied to the synthetic fibers using ultrasound and then formed by laser evaporation of the aqueous dispersion component. The structure of the nanocomposite implants was studied by optical and atomic force microscopy. Composite implant based on single-walled CNTs (SWCNTs) contains pores with a diameter of 10-20 nm, and based on multi-walled CNTs (MWCNTs)-40-60 nm. We conducted in vitro studies of proliferative activity of human fibroblast cells (HFb) during their colonization on the surface of the implant and into the space between synthetic fibers. The highest value of the HFb proliferation was observed on the implant based on MWCNTs with a large pore size and amounted to 55.435 pcs., in contrast to the implant based on SWCNTs (54.931 pcs.) and control one (54.715 pcs.), as shown by fluorescence microscopy and MTT test. A histological study of the interaction of the nanocomposite implant implanted into rabbit knee joint with bone canal was carried out. The bone germination in the implantation area at 2, 4 and 8 weeks after surgery was shown.

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“…The average SWCNTs diameter was ~ 1.4-1.6 nm, its length was -0.5-1.5 μm, its surface area was ~ 400 m 2 /g. The MWCNTs diameter was ~ 50-60 nm, length -3-7 μm, the wall thickness -15-20 nm, the core space diameter -10-20 nm, the specific surface area -90-120 m 2 /g [4]. The intense radiation absorption followed by heating of single CNTs and its conglomerates occurs during the laser radiation of the water-albumin CNTs dispersion.…”

Section: Methodsmentioning

confidence: 99%

Study of the structure of 3-D composites based on carbon nanotubes in bovine serum albumin matrix by X-ray microtomography

Ignatov¹,

Zhurbina²,

Gerasimenko

2017

J. Phys.: Conf. Ser.

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Laser nanostructuring 3-D bioconstruction based on carbon nanotubes in a water matrix of albumin

Cited by 10 publications

References 10 publications

Laser structuring of carbon nanotubes in the albumin matrix for the creation of composite biostructures

Laser structuring of carbon nanotubes in the albumin matrix for the creation of composite biostructures

Laser Technology of Designing Nanocomposite Implants of the Knee Ligaments

Study of the structure of 3-D composites based on carbon nanotubes in bovine serum albumin matrix by X-ray microtomography

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