The efficiency of devices for biomedical applications, including tissue engineering and neuronal stimulation, heavily depends on their biocompatibility and performance level. Therefore, it is important to find adequate materials that meet the necessary requirements such as (i) being intrinsically compatible with biological systems, (ii) providing a sufficient electronic conductivity that promotes efficient signal transduction, (iii) having “soft” mechanical properties comparable to biological structures, and (iv) being degradable in physiological solution. We have developed organic conducting biocompatible single-walled carbon nanotubes (SWCNT) composites based on bovine serum albumin, carboxymethylcellulose, and acrylic polymer and investigated their properties, which are relevant for biomedical applications. This includes ζ-potential measurements, conductivity analyses, and SEM micrographs, the latter providing a local analysis of SWCNT distribution in the base material. We observed the development of the electrical conductivity of the SWCNT composites exposed to 1 mM KCl electrolyte for 40 days, representing a high stability of the samples. The conductivity of samples reaches 1300 S/m for 0.45 wt.% nanotubes. Moreover, we demonstrated the biocompatibility of the composites via cultivating fibroblast cell culture. Finally, we showed that composite coating results in the longer lifespan of cells on the surface. Overall, the SWCNT-based conductive composites might be a promising material for extended biomedical applications.
Purpose: to study the cytological, immunological and antiviral effects of the HILOPARIN-KOMOD® drug in vitro.Material and methods.We used transplantable cultures of normal cells of the human Chang conjunctiva, and the kidney cells of the Vero monkeys. The cytotoxic effect of the HILOPARIN-KOMOD® was determined by the effect on the cell viability, and by optical density (OP) of the monolayer of the Chang conjunctiva cell culture using the enzyme immunoassay (ELISA) with MTT. The effect of the drug on the functional activity of conjunctival cells was evaluated by the production of cytokines at the level of their in vitro transcription. The antiviral effect of the drug HILOPARIN-KOMOD® was studied on the Vero cell line infected with herpes simplex virus type 1 (HSV-1) and HSV type 2 (HSV-2). The viral activity of HSV-1 and HSV-2 and the antiherpetic effect after the drug was evaluated by polymerase chain reaction (PCR). Results.No significant cytotoxic effect on the metabolism of conjunctival cells of the preparation HILOPARINKOMOD ® in dilutions from 1/2 to 1/2048 was revealed (in comparison with the control). The effect of the drug in dilutions of 1/40 and 1/1000 on the culture of the conjunctival cells resulted in suppression of the production of interferon λ-1 mRNA (IFNλ-1) and IFNλ-2 mRNA compared to the control. In the dilution of preparation 1/1000, the production of mRNA of interleukin-6 (IL-6) was not revealed with simultaneous presence of IL-10 mRNA. In different dilutions of the drug, the number of copies of HSV-1 virus DNA decreased in comparison with the control in all cases, the greatest antiviral effect was achieved in a 1: 2 dilution. The effect of the preparation HILOPARIN-KOMOD® on the culture of cells infected with HSV-2 in dilutions of 1: 2 and 1: 5 led to a decrease in the level of viral replication by 300 and 40 times, respectively.The conclusion.The drug HILOPARIN-KOMOD® has an anti-inflammatory effect that manifests itself in suppressing the synthesis of the mRNA of the acute phase proinflammatory cytokine IL-6 and stimulating the production of anti-inflammatory IL-10 mRNA, without having a cytotoxic effect on conjunctival cells. The proven antiviral effect of the drug makes it possible to recommend the inclusion of HILOPARIN-KOMOD® in the scheme of therapy of patients with viral ophthalmopathology.
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