Laser microdissection-based expression analysis of key genes involved in muscle regeneration in mdx mice

Marotta, Mario; Sarria, Yaris; Ruiz-Roig, Clàudia; Munell, Francina; Roig-Quilis, Manuel

doi:10.1016/j.nmd.2007.05.007

Cited by 23 publications

(29 citation statements)

References 63 publications

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“…These findings concur with previous studies, where the mRNA expression of MyoD1 showed basal levels in recently regenerated mdx hind-limb muscles [25,[27][28]. These studies, as well as our findings, permit the suggestion that the mRNA expression of MyoD1 may be elevated in the early stages of regeneration during myoblast determination [31], and during active regeneration, and that it decreases to its basal levels when regeneration is terminated.…”

Section: Discussionsupporting

confidence: 93%

“…However, these factors have been shown to increase during regeneration. In many recent studies, increased myogenin mRNA levels in mdx gastrocnemius muscles [25] or in the tibialis anterior [27], and increased myogenin expression on the protein level in soleus muscles [28] (90-to 100-day old mdx mice) were detected in the regeneration stages. Interestingly, the functional myogenic gene seems to be essential for survival.…”

Section: Discussionmentioning

confidence: 99%

“…It was also found that these changes were accompanied by intensive regeneration. Normal skeletal muscles, where there is no intensive regeneration, do not show increases in growth regulatory factors [25][26]. However, these factors have been shown to increase during regeneration.…”

Section: Discussionmentioning

confidence: 99%

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The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

Spassov

Gredes

Gedrange

et al. 2011

Cellular and Molecular Biology Letters

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Abstract:The activities of myogenic regulatory factors (MRF) and muscle growth factors increase in muscle that is undergoing regeneration, and may correspond to some specific changes. Little is known about the role of MRFs in masticatory muscles in mdx mice (the model of Duchenne muscular dystrophy) and particularly about their mRNA expression during the process of muscle regeneration. Using Taqman RT-PCR, we examined the mRNA expression of the MRFs myogenin and MyoD1 (myogenic differentiation 1), and of the muscle growth factors myostatin, IGF1 (insulin-like growth factor) and MGF (mechanogrowth factor) in the masseter, temporal and tongue masticatory muscles of mdx mice (n = 6 to 10 per group). The myogenin mRNA expression in the mdx masseter and temporal muscle was found to have increased (P < 0.05), whereas the myostatin mRNA expressions in the mdx masseter (P < 0.005) and tongue (P < 0.05) were found to have diminished compared to those for the controls. The IGF and MGF mRNA amounts in the mdx mice remained unchanged. Inside the mdx animal group, gender-related differences in the mRNA expressions were also found. A higher mRNA expression of myogenin and MyoD1 in the mdx massterer and temporal muscles was found in females in comparison to males, and the level of myostatin was higher in the masseter and tongue muscle (P < 0.001 for all comparisons). Similar gender-related differences were also found within the control groups. This study reveals the intermuscular differences in the mRNA expression pattern of myogenin and myostatin in mdx mice. The existence of these differences implies that dystrophinopathy affects the skeletal muscles differentially. The finding of gender-related differences in the mRNA expression of the examined factors may indicate the importance of hormonal influences on muscle regeneration.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

Spassov

Gredes

Gedrange

et al. 2011

Cellular and Molecular Biology Letters

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“…Together with our histological findings [22], the results indicate that mdx masseter and temporal muscles must be fully regenerated, because MyoD1 and myogenin expression in adult muscle increases only during regeneration or disuse [20]. Our findings concur with recent studies which revealed that MyoD1 and myogenin were expressed at basal levels in completely regenerated fibers of mdx gastrocnemius [13] and mdx soleus muscle [7].…”

Section: Ergebnissesupporting

confidence: 91%

“…Since the evidence suggests that MyoD1 and myogenin are extensively expressed during active regeneration in mdx hindlimb muscles [1,7,13], further experiments are being performed in our laboratory to examine their expression in 30-and 60-day-old mdx mice.…”

Section: Ergebnissementioning

confidence: 99%

Myogenic differentiation factor 1 and myogenin expression not elevated in regenerated masticatory muscles of dystrophic (mdx) mice

et al. 2011

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The Genetics and Development of Scoliosis

2010

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Laser microdissection-based expression analysis of key genes involved in muscle regeneration in mdx mice

Cited by 23 publications

References 63 publications

The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

Myogenic differentiation factor 1 and myogenin expression not elevated in regenerated masticatory muscles of dystrophic (mdx) mice

The Genetics and Development of Scoliosis

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