Laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals

Lone, Shabir Ahmad; Mohanty, T.K.; Kumaresan, A.; Bhakat, Mukesh

doi:10.12980/apjr.6.20170301

Cited by 7 publications

(6 citation statements)

References 66 publications

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“…The presence of opsins in the spermatozoa, the modification of the mitochondrial activity, and activation of plasma membrane receptors from the transient receptor potential (TRP) family of proteins have been suggested as possible mechanisms of action of the illumination on the sperm functionality [21][22][23][24][25]. However, in this study, these reported changes in the sperm parameters had no effect on the subsequent reproductive outcomes, measured in terms of farrowing rate and litter size under commercial conditions.…”

Section: Discussionmentioning

confidence: 59%

Photo Stimulation of Seminal Doses with Red LED Light from Duroc Boars and Resultant Fertility in Iberian Sows

Crespo

Martínez²,

Gadea

2021

Animals

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In pigs, it has been reported that increased farrowing rates and litter size have been induced by photostimulating the seminal doses for artificial insemination with red LED light. As the reproductive characteristics, production system, and outcome parameters of Iberian breed pigs are different from other commercial breeds, the aim of this study was to evaluate the possible effect of illuminating seminal doses from Duroc boars with red LED light and the fertility outcomes of Iberian females. Semen samples were obtained from 38 fertile Duroc boars. Photostimulation of the artificial insemination (AI) seminal doses was carried out by illuminating the samples with a red LED for 10 min, followed by 10 min of darkness, and finally 10 additional minutes of red light. The fertility study was conducted on two commercial farms using multiparous Iberian sows (farm A, n = 824; farm B, n = 2131), that were randomly assigned to LED (L) or control (C) groups. No differences were found between L and C groups in both farms (p > 0.05) for parity, pregnancy rate, duration of pregnancy, farrowing rate, and litter size (total, alive, and stillborn piglets). Farrowing rates in farm A were 88.8% (n = 383) for control and 89.6% (n = 441, p = 0.67) for the LED group. In farm B, farrowing rates were C:90.5% (n = 1030) and L: 90.1% (n = 1101, p = 0.48). In farm A, total born piglets were 8.69 ± 0.11 for C and 8.71 ± 0.11 for L (p = 0.87). In farm B, the results were 8.72 ± 0.7 for C and 8.70 ± 0.06 (p = 0.82) for L. Under the production conditions for the Iberian breed, the photostimulation with red LED light using Duroc pig seminal doses was not effective in improving the fertility of Iberian sows.

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Section: Discussionmentioning

confidence: 59%

Photo Stimulation of Seminal Doses with Red LED Light from Duroc Boars and Resultant Fertility in Iberian Sows

Crespo

Martínez²,

Gadea

2021

Animals

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“…The most pronounced effect according to (30) was observed at energy dose of D = 6120 mJ cm À2 . It is important to note that the energy doses used by us to ensure the preservation of sturgeon sperm are about 34 times lower, which may be due to different photosensitivity of sperm cells from different species (3)(4)(5)29,36).…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, due to several reasons it is not possible to draw a conclusion from above data on the optimal wavelength of radiation, which would have the highest stimulatory effect on the sperm. First of all, the dependence of the photobiological effect on the origin of sperm among species is well known-when the radiation with equal parameters has a distinct effect on the spermatozoa of various animals or birds (3)(4)(5)29,36). Secondly, the data on the magnitudes of the photobiological effects obtained in various research groups are difficult to compare because of the individual characteristics of sperm cells, differing conditions of their storage, irradiation and estimates of the magnitude of the effect.…”

Section: •-mentioning

confidence: 99%

“…However, despite the actual absence of comparative studies on the effect of radiation with different wavelengths on the functional characteristics of spermatozoa under equal experimental conditions, it is increasingly believed in the literature (3)(4)(5)25,54) that the short-wavelength radiation can be more effective than radiation in the red spectral region as the short-wavelength radiation is better absorbed by cellular chromophores and more efficiently generates ROS (7,29,46,47,54). At the same time, studying the dependence of the biomodulatory effect on the wavelength of radiation is of interest both for elucidating the contribution of various photoacceptor molecules to the realization of the photobiological effect and for the practical application of photobiomodulation approaches in reproductive biotechnologies to increase the probability of oocyte fertilization by improving the preservation and motility of spermatozoa.…”

Section: •-mentioning

confidence: 99%

“…35 years after the publication of the first research results (1) concerning the effect of laser radiation in red spectral region on motility and velocity of human spermatozoa, the ability of lowintensity laser radiation in visible and near-infrared spectral regions to have a modulatory effect on the functional characteristics of human and animal sperm is practically not called into question (2)(3)(4)(5)(6)(7). Data indicating the absence of the effect of optical radiation on the functional characteristics of spermatozoa are single (8).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

Plavskiĭ

Микулич

Barulin

et al. 2020

Photochem & Photobiology

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A comparative study of the effect of low‐intensity laser radiation in green (λ = 532 nm) and red (λ = 632.8 nm) spectral regions at equal average irradiance (3 mW cm−2) on functional characteristics of Siberian sturgeon spermatozoa is carried out. Confirmation of the photobiomodulation effect of the radiation is obtained by analyzing spermatozoa motility, percentage of motile spermatozoa and fertilization rate. It is shown that, depending on the energy dose, the laser radiation in red and green spectral regions can have both stimulatory and inhibitory effects on spermatozoa motility. Contrary to popular belief that the short‐wavelength radiation has great prospects in reproductive biotechnologies (due to more efficient absorption of radiation by cellular chromophores and increased generation of ROS), convincing evidence of a more pronounced stimulatory effect of radiation in the red spectral region was obtained. For the first time, metal‐free porphyrins capable of acting as endogenous photosensitizers generating ROS were detected and identified in animal sperm. Using luminol‐dependent chemiluminescence, it is shown that the increased production of ROS capable of exerting an inhibitory effect on biological systems at high concentrations is among the possible reasons for reduction in the stimulatory effect of radiation when moving from red to green spectral region.

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Photobiomodulation therapy for male infertility

Zupin¹,

Pascolo²,

Luppi³

et al. 2020

Lasers Med Sci

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Laser irradiation effects and its possible mechanisms of action on spermatozoa functions in domestic animals

Cited by 7 publications

References 66 publications

Photo Stimulation of Seminal Doses with Red LED Light from Duroc Boars and Resultant Fertility in Iberian Sows

Photo Stimulation of Seminal Doses with Red LED Light from Duroc Boars and Resultant Fertility in Iberian Sows

Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

Photobiomodulation therapy for male infertility

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