Laser interference microscopy of amphibian erythrocytes: impact of cell volume and refractive index

Yusipovich, A. I.; Zagubizhenko, M. V.; Левин, Г. Г.; Platonova, A. A.; Parshina, E. Yu.; Grygorzcyk, R.; Максимов, Г. В.; Rubin, Andrey B.; Orlov, Sergei N.

doi:10.1111/j.1365-2818.2011.03516.x

Cited by 18 publications

(10 citation statements)

References 37 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, data obtained by this method might be also impacted by changes in the hydrophobic properties of intracellular milieu detected in apoptotic cells (55). Three-dimensional (3D) reconstruction of single-cell images by laser interference or holographic microscopy (109) cannot be employed for volume measurement because the average refractive index of the cytoplasm is affected by cell shrinkage (131). The calceinquenching technique assumes its homogenous distribution within intracellular milieu (117).…”

Section: Cell Volume Changes Triggered By Death Stimulimentioning

confidence: 99%

Cell volume and monovalent ion transporters: their role in cell death machinery triggering and progression

Orlov

Platonova

Hamet

et al. 2013

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Cell death is accompanied by the dissipation of electrochemical gradients of monovalent ions across the plasma membrane that, in turn, affects cell volume via modulation of intracellular osmolyte content. In numerous cell types, apoptotic and necrotic stimuli caused cell shrinkage and swelling, respectively. Thermodynamics predicts a cell type-specific rather than an ubiquitous impact of monovalent ion transporters on volume perturbations in dying cells, suggesting their diverse roles in the cell death machinery. Indeed, recent data showed that apoptotic collapse may occur in the absence of cell volume changes and even follow cell swelling rather than shrinkage. Moreover, side-by-side with cell volume adjustment, monovalent ion transporters contribute to cell death machinery engagement independently of volume regulation via cell type-specific signaling pathways. Thus, inhibition of Na(+)-K(+)-ATPase by cardiotonic steroids (CTS) rescues rat vascular smooth muscle cells from apoptosis via a novel Na(+)i-K(+)i-mediated, Ca(2+)i-independent mechanism of excitation-transcription coupling. In contrast, CTS kill renal epithelial cells independently of Na(+)-K(+)-ATPase inhibition and increased [Na(+)]i/[K(+)]i ratio. The molecular origin of [Na(+)]i/[K(+)]i sensors involved in the inhibition of apoptosis as well as upstream intermediates of Na(+)i/K(+)i-independent death signaling triggered by CTS remain unknown.

show abstract

Section: Cell Volume Changes Triggered By Death Stimulimentioning

confidence: 99%

Cell volume and monovalent ion transporters: their role in cell death machinery triggering and progression

Orlov

Platonova

Hamet

et al. 2013

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

“…was evaluated by recording the average phase height (average OPD-optical path difference), surface area and density [16][17][18][19][20][21][22][23][24][25][26].…”

Section: Methodsmentioning

confidence: 99%

Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

et al. 2015

View full text Add to dashboard Cite

We investigated human blood erythrocytes under oxidative stress in vitro and established a correlation between composition and state of lipids and changes in erythrocytes structure under induced oxidative stress. These changes may serve as an indicator of not only the erythrocyte state but of systemic processes that occur at the level of the whole organism, including various pathologies as well. We found that a pyrimidine derivative xymedon used in the present study is an effective inhibitor of oxidative processes. Xymedon may be useful as an antioxidant for preserving the structural and functional characteristics of the erythrocytes in the treatment of organisms exposed to physical and toxic factors causing oxidative stress.

show abstract

“…Further constraints include photodynamic damage to cells during longer exposure to laser light. For comprehensive review, see [20,21].…”

Section: Introductionmentioning

confidence: 99%

Role of cytoskeleton network in anisosmotic volume changes of intact and permeabilized A549 cells

Platonova

Ponomarchuk

Boudreault

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Recently we found that cytoplasm of permeabilized mammalian cells behaves as a hydrogel displaying intrinsic osmosensitivity. This study examined the role of microfilaments and microtubules in the regulation of hydrogel osmosensitivity, volume-sensitive ion transporters, and their contribution to volume modulation of intact cells. We found that intact and digitonin-permeabilized A549 cells displayed similar rate of shrinkage triggered by hyperosmotic medium. It was significantly slowed-down in both cell preparations after disruption of actin microfilaments by cytochalasin B, suggesting that rapid water release by intact cytoplasmic hydrogel contributes to hyperosmotic shrinkage. In hyposmotic swelling experiments, disruption of microtubules by vinblastine attenuated the maximal amplitude of swelling in intact cells and completely abolished it in permeabilized cells. The swelling of intact cells also triggered ~10-fold elevation of furosemide-resistant (86)Rb+ (K+) permeability and the regulatory volume decrease (RVD), both of which were abolished by Ba2+. Interestingly, RVD and K+ permeability remained unaffected in cytocholasin/vinblastine treated cells demonstrating that cytoskeleton disruption has no direct impact on Ba2+-sensitive K+-channels involved in RVD. Our results show, for the first time, that the cytoskeleton network contributes directly to passive cell volume adjustments in anisosmotic media via the modulation of the water retained by the cytoplasmic hydrogel.

show abstract

Laser interference microscopy of amphibian erythrocytes: impact of cell volume and refractive index

Cited by 18 publications

References 37 publications

Cell volume and monovalent ion transporters: their role in cell death machinery triggering and progression

Cell volume and monovalent ion transporters: their role in cell death machinery triggering and progression

Study of correlation between state and composition of lipid phase and change in erythrocytes structure under induction of oxidative processes

Role of cytoskeleton network in anisosmotic volume changes of intact and permeabilized A549 cells

Contact Info

Product

Resources

About