Laser-induced forward transfer of biomolecules

Fernández-Pradas, J.M.; Colina, M.; Serra, P.; Domínguez, Jon Eunan Quinlivan; Morenza, J.L.

doi:10.1016/j.tsf.2003.11.154

Cited by 116 publications

(57 citation statements)

References 17 publications

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“…9 In a different work we showed that doublestranded DNA could be transferred from a liquid film by means of a conventional nanosecond laser with good adhesion onto treated glass substrates. 10 However, these results do not demonstrate the viability of LIFT for DNA microarray fabrication, since they do not grant that the amount of undamaged material adhered in each spot can provide enough signal intensity to be detected in a hybridization assay. In fact, a functional DNA microarray fabricated through LIFT allowing gene discrimination by means of conventional hybridization assays has not been reported yet.…”

mentioning

confidence: 89%

Preparation of functional DNA microarrays through laser-induced forward transfer

Serra

Colina

Fernández-Pradas

et al. 2004

Applied Physics Letters

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166

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A functional DNAmicroarray was prepared through the laser-induced forward transfer (LIFT) technique. In a first experiment, droplets of a buffer solution were spotted onto a substrate at different laser pulse energies. This allowed one to determine that uniform spots with a diameter as small as 40μm could be obtained. In a second experiment, a microarray containing two different human cDNAs and a negative control was spotted through LIFT and submitted to a hybridization assay. The obtained results demonstrated the full functionality of the microarray, which allowed us to prove the viability of LIFT for the production of DNAmicroarrays.Postprint (published version

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mentioning

confidence: 89%

Preparation of functional DNA microarrays through laser-induced forward transfer

Serra

Colina

Fernández-Pradas

et al. 2004

Applied Physics Letters

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166

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“…Since many solutions of interest are transparent to the laser radiation, an absorbing layer is usually placed between the donor film and its support. 7,8 The translation of both donor and receptor with respect to the laser beam allows printing micropatterns of any predefined two-dimensional geometry. The technique has also a high degree of spatial resolution: under appropriate irradiation conditions the material can be deposited in the form of single microdroplets, 9 which can have diameters as small as a few microns.…”

Section: Introductionmentioning

confidence: 99%

“…10 Moreover, LIFT can be used for printing different complex materials, such as inorganic inks or pastes, 11 organic polymers, 12 and even biological solutions. 8,[13][14][15] The feasibility of the technique for depositing these materials has been proved through the fabrication of diverse functional devices such as microbatteries, 11 solar cells, 16 organic light-emitting diodes, 17 or biosensors. 18,19 Such interesting features prompted several studies on the transfer process which takes place during the LIFT of liquids.…”

Section: Introductionmentioning

confidence: 99%

Time-resolved imaging of the laser forward transfer of liquids

Duocastella¹,

Fernández-Pradas²,

Morenza³

et al. 2009

Journal of Applied Physics

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136

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Time-resolved imaging is carried out to study the dynamics of the laser-induced forward transfer of an aqueous solution at different laser fluences. The transfer mechanisms are elucidated, and directly correlated with the material deposited at the analyzed irradiation conditions. It is found that there exists a fluence range in which regular and well-defined droplets are deposited. In this case, laser pulse energy absorption results in the formation of a plasma, which expansion originates a cavitation bubble in the liquid. After the further expansion and collapse of the bubble, a long and uniform jet is developed, which advances at a constant velocity until it reaches the receptor substrate. On the other hand, for lower fluences no material is deposited. In this case, although a jet can be also generated, it recoils before reaching the substrate. For higher fluences, splashing is observed on the receptor substrate due to the bursting of the cavitation bubble. Finally, a discussion of the possible mechanisms which lead to such singular dynamics is also provided.

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“…The feasibility of LIFT to print patterns of individual droplets has been extensively demonstrated [8,9,[12][13][14][15][16][17][18], and in addition, the mechanisms responsible for droplet formation have been widely investigated by time resolved imaging studies [19][20][21][22][23]. Moreover, the next step forward with respect to droplet deposition is the printing of defect-free continuous, uniform, and stable lines.…”

Section: Introductionmentioning

confidence: 99%

Deposition and characterization of lines printed through laser-induced forward transfer

et al. 2012

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The possibility of printing two-dimensional micro patterns of biomolecule solutions is of great interest in many fields of research in biomedicine, from cell-growth and development studies, to the investigation of the mechanisms of communication between cells. Although laser-induced forward transfer (LIFT) has been extensively used to print micrometric droplets of biological solutions, the fabrication of complex patterns depends on the feasibility of the technique to print micron-sized lines of aqueous solutions.In this study we investigate such a possibility through the analysis of the influence of droplet spacing of a water and glycerol solution on the morphology of the features printed by LIFT. We prove that it is indeed possible to print long and uniform continuous lines by controlling the overlap between adjacent droplets. We show how depending on droplet spacing, several printed morphologies are generated, and we offer, in addition, a simple explanation of the observed behavior based on the jetting dynamics characteristic of the LIFT of liquids.

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Laser-induced forward transfer of biomolecules

Cited by 116 publications

References 17 publications

Preparation of functional DNA microarrays through laser-induced forward transfer

Preparation of functional DNA microarrays through laser-induced forward transfer

Time-resolved imaging of the laser forward transfer of liquids

Deposition and characterization of lines printed through laser-induced forward transfer

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