2004
DOI: 10.1063/1.1787614
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Preparation of functional DNA microarrays through laser-induced forward transfer

Abstract: A functional DNAmicroarray was prepared through the laser-induced forward transfer (LIFT) technique. In a first experiment, droplets of a buffer solution were spotted onto a substrate at different laser pulse energies. This allowed one to determine that uniform spots with a diameter as small as 40μm could be obtained. In a second experiment, a microarray containing two different human cDNAs and a negative control was spotted through LIFT and submitted to a hybridization assay. The obtained results demonstrated… Show more

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Cited by 166 publications
(98 citation statements)
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“…8,[13][14][15] The feasibility of the technique for depositing these materials has been proved through the fabrication of diverse functional devices such as microbatteries, 11 solar cells, 16 organic light-emitting diodes, 17 or biosensors. 18,19 Such interesting features prompted several studies on the transfer process which takes place during the LIFT of liquids. These studies 9,10,14,15,20 were mainly focused on the analysis of the effects that different technological parameters have on the morphology of the transferred material.…”
Section: Introductionmentioning
confidence: 99%
“…8,[13][14][15] The feasibility of the technique for depositing these materials has been proved through the fabrication of diverse functional devices such as microbatteries, 11 solar cells, 16 organic light-emitting diodes, 17 or biosensors. 18,19 Such interesting features prompted several studies on the transfer process which takes place during the LIFT of liquids. These studies 9,10,14,15,20 were mainly focused on the analysis of the effects that different technological parameters have on the morphology of the transferred material.…”
Section: Introductionmentioning
confidence: 99%
“…2 In recent years, significant study has been directed towards extending the range of materials that can be deposited using LIFT; metals, 3 oxides, 2 superconductors, 4 DNA, 5 proteins, 6 fungal spores, 7 polycrystalline Si, 8 and various important electronic and sensing materials 9 have all been transferred. In contrast, efforts to reduce the minimum achievable deposition dimensions have received comparatively little attention.…”
mentioning
confidence: 99%
“…Ce procédé a été largement étudié ces dernières années et a démontré en particulier son intérêt pour imprimer des matériaux de natures aussi différentes que des nanotubes [2], des céramiques [3], ou des matériaux biologiques particulièrement fragiles (cellules [4], ADN [5]). …”
Section: Introductionunclassified