Laser-induced fluorescence detection of lead atoms in a laser-induced plasma: An experimental analytical optimization study

Laville, S.; Goueguel, Christian L.; Loudyi, H.; Vidal, François; Chaker, Mohamed; Sabsabi, Mohamad

doi:10.1016/j.sab.2009.03.021

Cited by 53 publications

(37 citation statements)

References 34 publications

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“…Consequently, the current combination of tissue preparation and a specific LIBS microscope configuration satisfies the requirements for the quantification of elements in most of biomedical applications, especially in terms of resolution and sensitivity. For specific application requiring higher sensitivity, the use of a second laser pulse tuned to excite specifically the element of interest would allow to improve significantly the performances in terms of sensitivity 44 . The major drawback of LIBS imaging is undeniably its invasive nature, although only a very small amount of biological material (<1 ng) is sampled during the measurement.…”

Section: Discussionmentioning

confidence: 99%

Laser spectrometry for multi-elemental imaging of biological tissues

Sancey

Motto-Ros

Busser

et al. 2014

Sci Rep

127

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An increasing interest has arisen in research focused on metallic and organic ions that play crucial roles in both physiological and pathological metabolic processes. Current methods for the observation of trace elements in biological tissues at microscopic spatial resolution often require equipment with high complexity. We demonstrate a novel approach with an all-optical design and multi-elemental scanning imaging, which is unique among methods of elemental detection because of its full compatibility with standard optical microscopy. This approach is based on laser-induced breakdown spectroscopy (LIBS), which allows the elements in a tissue sample to be directly detected and quantified under atmospheric pressure. We successfully applied this method to murine kidneys with 10 µm resolution and a ppm-level detection limit to analyze the renal clearance of nanoparticles. These results offer new insight into the use of laser spectrometry in biomedical applications in the field of label-free elemental mapping of biological tissues.

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Section: Discussionmentioning

confidence: 99%

Laser spectrometry for multi-elemental imaging of biological tissues

Sancey

Motto-Ros

Busser

et al. 2014

Sci Rep

127

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“…Optical and mass spectrometric methods have been developed for the direct determination of trace elements in solids. For optical methods, the representative techniques are X‐ray fluorescence (XRF), glow discharge optical emission spectrometry (GD‐OES), laser‐induced fluorescence (LIF), and laser‐induced breakdown spectrometry (LIBS) . Some drawbacks related to these optical techniques are intricate spectral interference, high background level, and relatively high limit of detection (LOD).…”

Section: Introductionmentioning

confidence: 99%

Improved detection sensitivity of elements in solids via laser postionization in laser desorption time‐of‐flight mass spectrometry

et al. 2018

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A newly constructed laser desorption (532 nm, 5 ns) and laser postionization (266 nm, 5 ns) time-of-flight mass spectrometer (LD-LPI-TOFMS) has been applied for improving the detection sensitivity of elements in solid samples. This method affords to acquire the information of the elemental impurities in solid standards as well as limit of detection (LOD) down to 10 g/g for some elements. Neutral atoms of solids are generated by low-irradiance laser desorption (< 10 W/cm ), followed by high-irradiance laser postionization (~ 10 W/cm ) of the desorbed atoms, facilitating to decouple the desorption and ionization processes in spatial and temporal domain. This non-interacting feature overcomes the discrimination between deteriorating spectral resolution at high irradiance (10 -10 W/cm ) and limited detectable elemental species and high LOD at low or medium irradiance (below 10 W/cm ). The utilization of originally "wasted" neutral atoms by laser postionization will help improve atom utilization and instrumental sensitivity. In this work, getting the utmost out of the consumed neutral atoms instead of an increment in sampling amounts is given attention with high priority for achieving high sensitivity and low LOD, which is especially useful on the occasions where very low sample consumption is desired.

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“…Most of the previous studies related to LIF of LA plumes were performed using pulsed lasers that only provide a fixed wavelength resonance excitation [16,22,23]. Pulsed laser excitation is typically delayed for some time after the initial LA pulse and will lead to preferential excitation of the lower energy state population at a specific time in the plasma evolution; however, it is possible for LIF signal saturation to occur due to depletion of the lower energy state atoms [24]. It has to be mentioned that, similar to the population density of an excited state, the lower energy state of any transition is also defined by a Boltzmann distribution.…”

Section: Introductionmentioning

confidence: 99%