Laser spectrometry for multi-elemental imaging of biological tissues

Sancey, Lucie; Motto-Ros, Vincent; Busser, Benoît; Kotb, Shady; Benoit, Jean‐Michel; Piednoir, Agnès; Lux, François; Tillement, Olivier; Panczer, G.; Yu, Jin

doi:10.1038/srep06065

Cited by 127 publications

(91 citation statements)

References 46 publications

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“…The instrument used to provide the LIBS images has been described in [12] and [13] (laser source was a Nd:YAG emitting 8 ns pulses at 1064 nm with a 100 Hz repetition rate; laser energy was reduced at typically 1.5 mJ; laser pulses were focused onto the sample by a 15× magnification objective resulting in a crater size close to 8 µm). The spatial distributions of Mg and Sr were found to be rather similar in the external layer (Figure 8a and 8c).…”

Section: Photonic Methods and Resultsmentioning

confidence: 99%

Photons and electrons for the study of a white veil covering some walls in prehistoric caves

et al. 2017

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Our research deals with the evolution of wall surfaces in prehistoric caves. The focus of this paper is dedicated to the structural and chemical characterization of a white concretion partially covering some walls in caves. In one of the caves, a non-ornate one which became a laboratory-cave (the Leye cave at Marquay, Dordogne, France) located in the Vézère valley, a set of physical methods has been proposed and tested on the first samples taken: SEM-EDXS, cathodoluminescence and laser-based techniques such as Raman spectroscopy and Laser Induced Breakdown Spectroscopy and LIBS. Thus two facies mainly composed of calcium carbonate crystals have been determined: moonmilk and coralloids. The identification of theses crystalline phases is the first step of an ambitious research project that plans to understand the development of unexpected layers on the cave walls of the famous ornate caves listed as part of the UNESCO cultural heritage sites. This first set of data provides good insight to the structure and the physico-chemical composition of the involved materials. Future works will be dedicated to bring knowledge about the facies chronology, the climatic conditions of environment (temperature, CO<sub>2 </sub>rate and air velocities) over a long period.

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Section: Photonic Methods and Resultsmentioning

confidence: 99%

Photons and electrons for the study of a white veil covering some walls in prehistoric caves

et al. 2017

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“…LIBS imaging, a novel advanced technique to specifically detect heavy metals/elements showed heterogeneous distribution of gold (RGD:AuNP) in the tumor tissue. 55,56 The spectral read-outs resonated with corresponding peaks for gold in the treated samples vs nontreated samples ( Figure 3I Other reported studies have shown that ≈10 nm-sized gold nanoparticles that accumulate in the liver (kupffer cells) are eventually cleared by the hepatobiliary pathways. 57 In addition to this, the collimated radiation setup that we employed largely reduces RGD:AuNP activation in off-target organs such as the liver.…”

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confidence: 80%

Nanoparticle-Mediated Tumor Vascular Disruption: A Novel Strategy in Radiation Therapy

Kunjachan

Detappe

Kumar

et al. 2016

International Journal of Radiation Oncology*Biology*Physics

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More than 50% of all cancer patients receive radiation therapy. The clinical delivery of curative radiation dose is strictly restricted by the proximal healthy tissues. We propose a dual-targeting strategy using vessel-targeted-radio-sensitizing gold nanoparticles and conformal-image guided radiation therapy to specifically amplify damage in the tumor neoendothelium. The resulting tumor vascular disruption substantially improved the therapeutic outcome and subsidized the radiation/nanoparticle toxicity, extending its utility to intransigent or nonresectable tumors that barely respond to standard therapies. Graphical abstract KeywordsGold nanoparticles; image-guided radiation therapy; endothelial radiation damage; tumor vascular disruption Nanoparticles for radiation therapy have been an active area of research for several decades in oncology. More than 50% of cancer patients receive therapeutic radiation at some stage of their treatment course. Although highly effective for inflicting cellular damage, the specificity of radiation therapy is mainly derived from the geometric restriction of radiation beams. Sparing of healthy tissues and organs from radiation can be particularly challenging when treating tumors that are located in deep-seated anatomical locations. A strategy to intensify the tumor damage without adding additional risk to the healthy tissue is extremely advantageous in the clinic.Multifunctional metallic nanoparticles have excellent potential as radiosensitizing agents primarily due to the superior interaction cross-section for high-z-elements when irradiated with low-energy X-rays. 1 This interaction results in the emission of short-range photoelectrons and Auger electrons, which can impose damage to the tumor cellular or subcellular structures. 2,3 Gold nanoparticles (AuNP) are of interest in radiation therapy due to its high k-edge (≈81 keV) and biocompatibility. In contrast to i.v. administered radioactive probes, gold is nontoxic in moderate quantities. [4][5][6] By specifically targeting AuNP to malignant tumor cells using "enhanced permeability and retention" (EPR) driven passive or peptide/antibody-mediated active tumor targeting, radiation damage can be invoked to the tumor cells upon irradiation. [7][8][9][10][11][12][13][14][15][16] Several preclinical studies have demonstrated this in different preclinical tumors, including prostate, breast, head and neck, cervical, sarcoma, glioblastoma, colorectal, and melanoma. 14,[17][18][19][20][21][22][23][24][25] Previous studies have examined the potential for a classic clonogenic effect in cancer cells leading to improved radiotherapeutic efficacy. To this end, EPR-mediated passive targeting has been used to attain high AuNP deposition in the tumor cells. Although there are several benefits attributed to passive tumor targeting, recent preclinical and clinical studies have shown that EPR-mediated passive tumor targeting was significantly less efficient (≈2-fold) in slow-growing animal pancreatic adenocarcinoma (PDAC) models versus fast...

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“…The imaging capability of Laser-induced breakdown spectroscopy (LIBS) represents an attractive way of development for the technique, with innumerable applications in various fields, such as geology [1][2][3], industry [4][5][6][7][8], surface science [9,10] and biology [11][12][13][14][15]. In LIBS imaging, laser-induced plasma are generated continuously while scanning the sample surface over the region of interest.…”

Section: Introductionmentioning

confidence: 99%

“…This approach has many advantages such as multielemental capability, ease in use, and operation at atmospheric pressure. This is furthermore the only all-optical technique providing space-resolved elemental information with a ppm-scale sensitivity and a µm-range resolution [12]. Another asset of the technique lies in its acquisition speed.…”

Section: Introductionmentioning

confidence: 99%

Quantitative elemental imaging of heterogeneous catalysts using laser-induced breakdown spectroscopy

Trichard

Sorbier

Moncayo

et al. 2017

Spectrochimica Acta Part B: Atomic Spectroscopy

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Nowadays, the use of catalysis is implied in almost all industrial process. Their use allows improving productivity, synthesis yields and waste treatment as well as decreasing the energy costs. The increasingly stringent requirements in terms of reaction selectivity and environmental standards impose an ever more accurate knowledge and control of their operations. However, the characterization techniques struggle to develop and often require equipment with high complexity.In this paper, we demonstrate a novel elemental approach with an all-optical design allowing quantitative space-resolved analysis to be performed with ppm-scale limit of quantification and µm-scale resolution. This approach, based on laser-induced breakdown spectroscopy (LIBS), is distinguished by its simplicity of use, all-optical design, and speed of operation. This work was conducted on palladium-based porous alumina catalyst, used for the selective hydrogenation process in the field of petrochemistry. We report an exhaustive study on the quantification capability of this technique with the possibility to perform imaging measurement over a large dynamical range, typically from few ppm to %. These results offer new insight into the use of LIBS imaging in the industry and paves the way for innumerable applications.2

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Laser spectrometry for multi-elemental imaging of biological tissues

Cited by 127 publications

References 46 publications

Photons and electrons for the study of a white veil covering some walls in prehistoric caves

Photons and electrons for the study of a white veil covering some walls in prehistoric caves

Nanoparticle-Mediated Tumor Vascular Disruption: A Novel Strategy in Radiation Therapy

Quantitative elemental imaging of heterogeneous catalysts using laser-induced breakdown spectroscopy

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