Laser diode thermal desorption mass spectrometry for the analysis of quinolone antibiotic residues in aquacultured seafood

Lohne, Jack J.; Andersen, Wendy C.; Clark, Stephen Nowland; Turnipseed, Sherri B; Madson, Mark R.

doi:10.1002/rcm.6414

Cited by 23 publications

(15 citation statements)

References 37 publications

(67 reference statements)

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“…They discovered that the residence time in the APCI region which is controlled by the carrier gas flow rate had an influence on the signal intensity. Furthermore, maximal signal intensity at a carrier gas flow rate of 3 L min −1 was also observed from other groups optimizing the LDTD-APCI parameters indicating that the carrier gas flow rate is not compound dependent but rather a combination of physical factors which influence the ionization efficiency [13,14,18]. The optimal corona discharge current Fig.…”

Section: Ldtd-apci Parameters Optimizationsupporting

confidence: 62%

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Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

et al. 2014

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A sensitive and ultra-fast method utilizing the laser diode thermal desorption ion source using atmospheric pressure chemical ionization coupled to tandem mass spectrometry (LDTD-APCI-MS/MS) was developed for the quantitative analysis of BKM120, an investigational anticancer drug in human plasma. Samples originating from protein precipitation (PP) followed by salting-out assisted liquid-liquid extraction (SALLE) were spotted onto the LazWell™ plate prior to their thermal desorption and detection by tandem mass spectrometry in positive mode. The validated method described in this paper presents a high absolute extraction recovery (>90 %) for BKM120 and its internal standard (ISTD) [D8]BKM120, with precision and accuracy meeting the acceptance criteria. Standard curves were linear over the range of 5.00 to 2000 ng mL(-1) with a coefficient of determination (R (2)) >0.995. The method specificity was demonstrated in six different batches of human plasma. Intra- and inter-run precision as well as accuracy within ±20 % at the lower limit of quantification (LLOQ) and ±15 % (other levels) were achieved during a three-run validation for quality control (QC) samples. The post-preparative stability on the LazWell™ plate at room temperature was 72 h and a 200-fold dilution of spiked samples was demonstrated. The method was applied successfully to three clinical studies (n = 847) and cross-checked with the validated LC-ESI-MS/MS reference method. The sample analysis run time was 10 s as compared to 4.5 min for the current validated LC-ESI-MS/MS method. The resultant data were in agreement with the results obtained using the validated reference LC-ESI-MS/MS assay and the same pharmacokinetic (PK) parameters were calculated for both analytical assays. This work demonstrates that LDTD-APCI-MS/MS is a reliable method for the ultra-fast quantitative analysis of BKM120 which can be used to speed-up and support its bioanalysis in the frame of the clinical trials.

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Section: Ldtd-apci Parameters Optimizationsupporting

confidence: 62%

“…The MS system was connected to a HTS PAL auto-sampler from CTC Analytics AG (Zwingen, Switzerland) and to an Agilent 1200 pump system (Wilmington, DE, USA). Chromatographic separation was performed at a flow rate of 400 μL min −1 on an Ascentis Express C 18 Table S1 in the Electronic Supplementary Material (ESM).…”

Section: Lc-esi-ms/ms (Reference Method)mentioning

confidence: 99%

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

et al. 2014

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“…The MDL was 1.7, 2.6, 4.4 ng/g for FLU, OXO and NAL, respectively. This method was found to meet many of the drug residue analysis requirements in shrimp tissue samples, using a single solvent extraction step, resulting in decreased sample analysis and increased sample throughput [36].…”

Section: Extraction Of Quinolonesmentioning

confidence: 99%

On the Extraction of Antibiotics from Shrimps Prior to Chromatographic Analysis

et al. 2016

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Abstract:The widespread use of antibiotics in veterinary practice and aquaculture has led to the increase of antimicrobial resistance in food-borne pathogens that may be transferred to humans. Global concern is reflected in the regulations from different agencies that have set maximum permitted residue limits on antibiotics in different food matrices of animal origin. Sensitive and selective methods are required to monitor residue levels in aquaculture species for routine regulatory analysis. Since sample preparation is the most important step, several extraction methods have been developed. In this review, we aim to summarize the trends in extraction of several antibiotics classes from shrimps and give a comparison of performance characteristics in the different approaches.

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“…Veterinary drug residue analysis of meat and seafood products is an important part of national regulatory agency food safety programs to ensure that consumers are not exposed to potentially dangerous substances [1]. Toussaint et al (2005) established a method for determination of quinolone antibiotic residues in pig kidney using liquid chromatography-tandem mass spectrometry [2].…”

Section: Introductionmentioning

confidence: 99%

A Review of Antibiotic Residue Determination in Aquatic Products

Mo¹,

Huang²,

Mingxing³

et al. 2017

Proceedings of the 2017 International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2017)

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Abstract. In recent years, with the development of fisheries, the aquaculture industry has developed rapidly and has become an important part of our country economy. At the same time more and more aquatic products is being used by people while the abuse of antibiotics is growing uncontrolled. This paper summarizes the detection methods of various kinds of antibiotics in aquatic products, as well as the method of spiked recovery, detection limit, quantitative limit, and puts forward the corresponding countermeasures proposal of controlling the abuse of antibiotics.

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Laser diode thermal desorption mass spectrometry for the analysis of quinolone antibiotic residues in aquacultured seafood

Cited by 23 publications

References 37 publications

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

On the Extraction of Antibiotics from Shrimps Prior to Chromatographic Analysis

A Review of Antibiotic Residue Determination in Aquatic Products

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