laser collapsing of blastocysts prior to vitrification leads to better embryonic survival and improved overall IVF cycle outcome

Fields, R.; Werland, H.; Nguyen, J.; Sieren, K.R.; Turner, T.; Silverberg, Kaylen M.

doi:10.1016/j.fertnstert.2013.07.1680

Cited by 2 publications

(2 citation statements)

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“…Several papers whose authors experimented the application of the laser collapse technique in expanded blastocysts demonstrated its efficiency, with improvement in survival rate, pregnancy rate and implantation rate, reducing cancellations of transfers after thawing. 12,20,31 On the other hand, in the research conducted by Marcos et al, 32 the authors affirm that the collapse reduced the implantation potential of the transferred blastocysts. They reported that the collapse could act adversely in the implantation, either because it affects the quality of the embryo or by directly reducing its implantation capacity resulting in lower rates of clinical pregnancy.…”

Section: Discussionmentioning

confidence: 93%

Laser collapse performance in blastocysts applied before vitrification

Macedo¹,

Oliveira²,

Gomes³

et al. 2018

Hum. Reprod. Arch.

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The objetive of the present study was to investigate the effect of the reduction of blastocele fluid before vitrification with the application of collapsing and the effects of this procedure on the expected results of in vitro fertilization protocols. Methods: A retrospective casecontrol study was conducted with patients who sought assisted fertilization services from January 2015 to October 2017 at Reproferty Clinic. After submission to the ethics committee, data from 106 patients were divided into two groups: 42 cases represented by those who had artificially collapsed blastocysts per laser pulse before vitrification and 64 controls corresponding to patients where artificial shrinkage of expanded blastocysts was not performed. By statistical analysis, the means and standard deviation of each group were obtained, which were compared by the Mann-Whitney test, at a 95% significance level. Results: the results showed that there was statistical significance comparing the group of cases and controls in the parameters related to embryo freezing, survival and transference as well as to the rates of positive beta human chorionic gonadotropin, clinical pregnancy and live births. Conclusions: the study demonstrated evidence that vitrification after artificial laser collapse of the blastocyst is safe, effective, and more efficient than vitrification of non-collapsed blastocysts in vitro fertilization protocols, however further studies are needed to evaluate the efficiency of artificial shrinkage in routine protocols.

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Section: Discussionmentioning

confidence: 93%

Laser collapse performance in blastocysts applied before vitrification

Macedo¹,

Oliveira²,

Gomes³

et al. 2018

Hum. Reprod. Arch.

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“…It is well known that the efficiency of blastocyst vitrification depends on the expansion stage of fluid-filled cavity [1,2,3,7]. While expanded blastocysts are more vulnerable to ice formation, early blastocysts have better post thaw survival rate due to the small amount of blastocoeli fluid [5].…”

Section: Introductionmentioning

confidence: 99%

Quality of Intact and Artificially Collapsed Human Blastocysts after Vitrification

Nenkova¹,

Yaneva²,

Dimitrova³

et al. 2022

AMA

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It is well known that the survival rate of embryos after vitrification depends on their expansion stage. We aimed to compare behavior of early (n=31) and expanded blastocysts (n=83) after vitrification. Expanded Day 5 embryos were divided into two groups -artificially collapsed (n=38) and intact (n=45). All blastocysts selected for vitrification were Day 5, classified into three groups -excellent, good and average quality. Thawed blastocysts were cultivated for at least 3 hours before embryo transfer. Assessment of their vitality and re-expansion was conducted every hour. Our study showed that mechanical collapse of blastocoele through micropipette puncture has a positive effect on embryo survival rate. The greatest survival rate (96.8%) and the fastest re-expansion were observed in early blastocysts. 35 (92.1%) survived thawing in the group of deflated blastocysts, while 39 embryos (86.6%) survived in the group of untreated before vitrification.

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laser collapsing of blastocysts prior to vitrification leads to better embryonic survival and improved overall IVF cycle outcome

Cited by 2 publications

References 0 publications

Laser collapse performance in blastocysts applied before vitrification

Laser collapse performance in blastocysts applied before vitrification

Quality of Intact and Artificially Collapsed Human Blastocysts after Vitrification

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