Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis

Casson, Stuart A.; Spencer, Matthew W.B.; Walker, K. P.; Lindsey, Keith

doi:10.1111/j.1365-313x.2005.02355.x

Cited by 184 publications

(180 citation statements)

References 59 publications

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“…BBM is expressed during the early stages of in vitro and seed embryogenesis, and in the seedling root meristem (Boutilier et al 2002;Casson et al 2005;Nawy et al 2005). Ectopic BBM expression in Arabidopsis is suYcient to induce spontaneous somatic embryogenesis and shoot development from seedlings and explants, and its expression in older tissues such as leaves and Xowers induces pleiotropic morphological alterations.…”

Section: Discussionmentioning

confidence: 99%

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Srinivasan

Liu

Heidmann

et al. 2006

Planta

143

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Gain-of-function studies have shown that ectopic expression of the BABY BOOM (BBM) AP2/ ERF domain transcription factor is suYcient to induce spontaneous somatic embryogenesis in Arabidopsis (Arabidopsis thaliana (L.) Heynh) and Brassica napus (B. napus L.) seedlings. Here we examined the eVect of ectopic BBM expression on the development and regenerative capacity of tobacco (Nicotiana tabacum L.) through heterologous expression of Arabidopsis and B. napus BBM genes. 35S::BBM tobacco lines exhibited a number of the phenotypes previously observed in 35S::BBM Arabidopsis and B. napus transgenics, including callus formation, leaf rumpling, and sterility, but they did not undergo spontaneous somatic embryogenesis. 35S::BBM plants with severe ectopic expression phenotypes could not be assessed for enhanced regeneration at the seedling stage due to complete male and female sterility of the primary transformants, therefore fertile BBM ectopic expression lines with strong misexpression phenotypes were generated by expressing a steroid-inducible, posttranslationally controlled BBM fusion protein (BBM:GR) under the control of a 35S promoter. These lines exhibited spontaneous shoot and root formation, while somatic embryogenesis could be induced from in-vitro germinated seedling hypocotyls cultured on media supplemented with cytokinin. Together these results suggest that ectopic BBM expression in transgenic tobacco also activates cell proliferation pathways, but diVerences exist between Arabidopsis/B. napus and N. tabacum with respect to their competence to respond to the BBM signalling molecule.

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Section: Discussionmentioning

confidence: 99%

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Srinivasan

Liu

Heidmann

et al. 2006

Planta

143

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show abstract

“…The BABY BOOM (BBM) AP2/ERF domain protein is a seed and root-meristem expressed transcription factor that was identified as marker for embryo development in Brassica napus microspore-derived embryo cultures (Boutilier et al 2002), as a gene showing preferential expression in the basal region of the Arabidopsis embryo (Casson et al 2005), and as an auxin-inducible root expressed gene in Medicago truncatula (Imin et al 2006). Ectopic expression of BBM in Arabidopsis primarily induces spontaneous somatic embryo formation from seedlings, although ectopic shoots and callus also develop at a lower frequency (Boutilier et al 2002).…”

Section: Introductionmentioning

confidence: 99%

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Passarinho¹,

Ketelaar²,

Xing³

et al. 2008

Plant Mol Biol

104

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Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.

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“…5). LCM technology has been used successfully in rice (Oryza sativa), maize (Zea mays), tobacco, soybean, and Arabidopsis in which a variety of plant tissues and cell types have been isolated and studied, including those in an early Arabidopsis embryo (Asano et al, 2002;Kerk et al, 2003;Nakazono et al, 2003;Casson et al, 2005;Klink et al, 2005;Sanders et al, 2005;Spencer et al, 2007).…”

Section: Using Soybean To Identify Genes Required To Make a Seedmentioning

confidence: 99%

Using Genomics to Study Legume Seed Development

Wagmaister²,

Kawashima³

et al. 2007

Plant Physiology

148

157

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Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis

Cited by 184 publications

References 59 publications

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Using Genomics to Study Legume Seed Development

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