Laser-Assisted aPDT Protocols in Randomized Controlled Clinical Trials in Dentistry: A Systematic Review

Mylona, Valina; Anagnostaki, Eugenia; Parker, Steven; Cronshaw, Mark; Lynch, Edward; Grootveld, Martin

doi:10.3390/dj8030107

Cited by 15 publications

(19 citation statements)

References 85 publications

(144 reference statements)

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“…Recently, aPDT has been introduced as an alternative therapy for preventing and treating dental caries via control the formation of the microbial bio lm and controlling the incidence of microbial pathogens without the development of resistance [29][30][31] . Several variables such as the type and concentration of photosensitizers, light sources, irradiation parameters, and physiologic condition of the microorganisms studied must be considered for the effectiveness of aPDT 32 .…”

Section: Discussionmentioning

confidence: 99%

Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

Pourhajibagher

Bahador

2021

Preprint

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The aim of this study was to evaluate the anti-biofilm and anti-metabolic activities of zeolite-zinc oxide nanoparticles (Zeo\ZnONPs)-based antimicrobial photodynamic therapy (aPDT) against pre-formed polymicrobial biofilms on the orthodontic brackets, as well as, assess the remineralization efficacy on polymicrobial biofilms induced enamel lesions. Following synthesis and characterization of Zeo\ZnONPs, cell cytotoxicity, hemolytic effect, and intracellular reactive oxygen species (ROS) production were determined. The anti-biofilm and anti-metabolic activities of aPDT using different concentrations of Zeo\ZnONPs were investigated. Microhardness tester and DIAGNOdent Pen were used to evaluate the changes of remineralization degree on the treated enamel slabs duration one and three months. No significant cytotoxicity and erythrocyte hemolysis were observed in treated cells with Ze\ZnONPs. When irradiated, suggesting that the Ze\ZnONPs were photoactivated, generating ROS and leading to reduce dose-dependently the cell viability and metabolic activity of polymicrobial biofilms. Also, the enamel surface microhardness value of exposed enamel showed a steady increase with the concentration of Zeo\ZnONPs. No statistically significant differences were shown between aPDT and sodium fluoride varnish as the control group. Overall, Zeo\ZnONPs-based aPDT with the greatest remineralization efficacy of enamel surface can be used as an anti-biofilm therapeutic method, which is involved with their potent ability to produce ROS.

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Section: Discussionmentioning

confidence: 99%

Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

Pourhajibagher

Bahador

2021

Preprint

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“…Especially in oral lichen planus (OLP), PDD might be a good treatment option [103][104][105]. PDD is also used as a therapy for (fungal) infections [106,107].…”

Section: Update Of Opmds Therapeutic Strategiesmentioning

confidence: 99%

Overview of Oral Potentially Malignant Disorders: From Risk Factors to Specific Therapies

Lorini

Bescós‐Atín

Thavaraj

et al. 2021

Cancers

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Oral squamous cell carcinoma (OSCC) is a very aggressive cancer, representing one of the most common malignancies worldwide. Oral potentially malignant disorders (OPMDs) regroup a variegate set of different histological lesions, characterized by the potential capacity to transform in OSCC. Most of the risk factors associated with OSCC are present also in OPMDs’ development; however, the molecular mechanisms and steps of malignant transformation are still unknown. Treatment of OSCC, including surgery, systemic therapy and radiotherapy (alone or in combination), has suffered a dramatic change in last years, especially with the introduction of immunotherapy. However, most cases are diagnosed during the advanced stage of the disease, decreasing drastically the survival rate of the patients. Hence, early diagnosis of premalignant conditions (OPMDs) is a priority in oral cancer, as well as a massive education about risk factors, the understanding of mechanisms involved in malignant progression and the development of specific and more efficient therapies. The aim of this article is to review epidemiological, clinical, morphological and molecular features of OPMDs, with the purpose to lay the foundation for an exhaustive comprehension of these lesions and their ability of malignant transformation and for the development of more effective and personalized treatments.

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“…The antimicrobial effect of a PS is strictly dependent on its physical properties (absorption peak (k max ), intensity of absorption (e max ), and quantum yield for singlet oxygen) and chemical parameters (lipophilicity/hydrophilicity balance (logP) and the presence of charged groups that determine the mechanism of cellular uptake). As a rule, hydrophilicity and the presence of charged groups are better for cellular uptake [13].…”

Section: Introductionmentioning

confidence: 99%

Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

Wiench

Nowicka

Pajączkowska

et al. 2021

IJMS

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(1) Background and the aim: The appropriate incubation time in the antimicrobial photodynamic therapy protocol seems to have a huge impact on the efficacy of this process. This is particularly important in relation to Candida strains, due to the size of these cells and the presence of the cell wall. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. krusei and C. parapsilosis using direct observation by optical microscopy, and to evaluate the efficacy of TBO-mediated aPDT on planktonic cells of these strains. (2) Methods: The microscopic evaluation consisted of taking a series of images at a magnification of 600× and counting the % of stained cells. The in vitro effect of TBO-mediated aPDT combined with a diode laser (635 nm, 400mW, 12 J/cm2, CW) on the viability of yeast cells with different incubation times was evaluated. (3) Results: The presence of TBO within the cytoplasm was observed in all tested Candida strains and at all microscopic evaluation times. However, the highest percentages of cells were stained at 7 and 10 min. The highest % reduction of CFU/mL after TBO-mediated aPDT against Candida was obtained for the strain C. albicans ATCC 10,231 and it was 78.55%. (4) Conclusions: TBO-mediated aPDT against Candida was effective in reducing the number of CFU/mL at all assessed incubation times. However, the most efficient period for almost all strains was 7–10 min.

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Laser-Assisted aPDT Protocols in Randomized Controlled Clinical Trials in Dentistry: A Systematic Review

Cited by 15 publications

References 85 publications

Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

Overview of Oral Potentially Malignant Disorders: From Risk Factors to Specific Therapies

Influence of Incubation Time on Ortho-Toluidine Blue Mediated Antimicrobial Photodynamic Therapy Directed against Selected Candida Strains—An In Vitro Study

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