Laser ablation based bioimaging with simultaneous elemental and molecular mass spectrometry: towards spatially resolved speciation analysis

Abstract

The first simultaneous elemental and molecular MS imaging approach based on laser ablation sampling was introduced for spatially resolved speciation analysis. The combination of the advantages of LA-ICP-MS such as low detection limits and high spatial resolution with information on the chemical identity promises not only localization of metals, but also identification of metal species in biological samples. Therefore, this novel technique opens up new possibilities to address complex challenges in life science research.

Elemental Bioimaging by Means of Fast Scanning Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry

“…Experiments were approved by a governmental committee on animal welfare (LaNUV, permission number A 140.2011) and performed in accordance with national animal protection guidelines. Perfusion, fixation, staining, and embedding of the tissue sample before sectioning to 3μm was done as described recently [33]. Lymph node samples were obtained from anonymized human patients as a waste product after surgeries and prepared to 8-μm-thick sections as described elsewhere [33].…”

“…Perfusion, fixation, staining, and embedding of the tissue sample before sectioning to 3μm was done as described recently [33]. Lymph node samples were obtained from anonymized human patients as a waste product after surgeries and prepared to 8-μm-thick sections as described elsewhere [33]. Human whole blood was taken from a healthy volunteer.…”

“…It dyes cytoplasmic material in red color [38]. Moreover, it has been recently of interest in elemental bioimaging as a reference marker for structural information [8,33,39].…”

Elemental bioimaging of platinum in mouse tissues by laser ablation-inductively coupled plasma-mass spectrometry for the study of localization behavior of structurally similar complexes

“…In this study, we used a third method, formalin-fixed paraffin embedding (FFPE) for sections of liver and kidney; thus, there were no restrictions in sample preparation and storage as mentioned for the previous two methods. The FFPE method is conventionally used to prepare soft tissues for histology study, and there are several examples of LA imaging using this kind of sample [38,39]. This method is more convenient as both storage and analysis can take place at room temperature.…”

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…Herdering et al 107 described the novel online combination of LA-ICP-MS and atmospheric pressure chemical ionization-MS for simultaneous spatially-resolved speciation analysis. Herdering et al 107 described the novel online combination of LA-ICP-MS and atmospheric pressure chemical ionization-MS for simultaneous spatially-resolved speciation analysis.…”