Larvicidal and repellent potential of Ageratum houstonianum against Culex pipiens

Hadidy, Doaa El; Sayed, Abeer M. El; Tantawy, Mona E. El; Alfy, Taha El; Farag, Shaimaa M.; Haleem, Doaa R Abdel

doi:10.1038/s41598-022-25939-z

Cited by 5 publications

(1 citation statement)

References 64 publications

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“…Adults were reared in wooden cages and they were fed everyday with 10% sucrose solution. For toxicological studies, the early third instar larvae were used 24 …”

Section: Methodsmentioning

confidence: 99%

Anti‐Culex pipiens activity of different pomegranate cultivars and determination of their bioactive compounds using LC‐MS profiling

Salem,

Farag,

Gad

et al. 2023

Phytochemical Analysis

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IntroductionPomegranate (Punica granatum L.) peels are rich in various bioactive compounds. Characterization of these compounds is crucial for the utilization of peel waste in industrial processing.ObjectiveThe study aimed (1) to establish and compare the metabolic profiles of the peel of seven pomegranate cultivars and (2) to identify bioactive compounds contributing to the larvicidal activity against the third instar larvae of Culex pipiens.Materials and MethodsUPLC‐ESI‐MS/MS was utilized to analyze peel methanol extracts of different pomegranate cultivars. The larvicidal activity was determined by calculating the larval mortality among the third instar larvae of C. pipiens. Multivariate data analysis was conducted to identify the metabolites that exhibited a larvicidal effect.ResultsA total of 24 metabolites, including hydrolyzable tannins, flavonoids, and alkaloids, were tentatively identified in both negative and positive ionization modes. The extract of cultivar ‘Black’ exhibited the most potent larvicidal effect with LC50 values of 185.15, 156.84, and 138.12 ppm/mL after 24, 48, and 72 h of treatment, respectively. By applying chemometric techniques, the larvicidal activity could be directly correlated to the bioactive compounds punicalagin, quercetin‐O‐rhamnoside, quercetin‐O‐pentoside, and galloyl‐HHDP‐glucose.ConclusionThe present study implemented UPLC‐ESI‐MS/MS and chemometric techniques as potential tools for metabolomics analysis and differentiation between peels of different pomegranate cultivars. In addition, cultivar ‘Black’ extract could be a promising natural insecticide against mosquitoes since it is rich in bioactive compounds with larvicidal activity.

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“…Adults were reared in wooden cages and they were fed everyday with 10% sucrose solution. For toxicological studies, the early third instar larvae were used 24 …”

Section: Methodsmentioning

confidence: 99%

Anti‐Culex pipiens activity of different pomegranate cultivars and determination of their bioactive compounds using LC‐MS profiling

Salem,

Farag,

Gad

et al. 2023

Phytochemical Analysis

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Chemical composition of four essential oils and their adulticidal, repellence, and field oviposition deterrence activities against Culex pipiens L. (Diptera: Culicidae)

Farag,

Moustafa,

Fónagy

et al. 2024

Parasitol Res

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Effective mosquito repellents can limit the transmission of vector-borne diseases to humans. Consequently, there is an urgent need to develop mosquito control strategies that prioritize eco-friendly and cost-effective repellents. Essential oils (EOs) have enormous potential for mosquito repellency. Here, cinnamon, basil, eucalyptus, and peppermint EOs were investigated for adulticide and repellency properties against Culex pipiens as well on the oviposition behavior of gravid females from laboratory (lab test) and field (field test) populations. Cinnamon oil was an effective oviposition deterrent regardless of the population and had high adulticidal activity with toxicity index of 75.00% at 24 h of exposure, relative to deltamethrin. In addition, it exhibited effective repellency at 98.01% and 71.22% at 6.67 and 1.71 µl/cm2, respectively. Peppermint oil had the least adulticidal activity with toxicity index of 6.2% at 24 h, and it resulted in low repellency at 70.90% and 50.64% at 6.67 and 1.71 µl/cm2, respectively. On average, basil and eucalyptus oils showed some adulticidal efficiency, repellency, and oviposition deterrent activity. For all treatments, the oviposition deterrent index values of gravid females from natural populations (field test) were lower than those from lab-reared (lab test) females. Different ratios of monoterpenoids, phenylpropanoids, and fatty acids in the EOs tested likely account for the activity variations observed. Our results suggest cinnamon, basil, eucalyptus, and peppermint EOs, which are widely available, economical, and eco-friendly, with good potential for mosquito control strategies.

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Larvicidal, Ovicidal, and Repellent Activities of Leucas stachydiformis (Hochst. ex Benth.) Briq Essential Oil against Anopheles arabiensis

Fikru,

Tolossa,

Lindemann

et al. 2024

Journal of Tropical Medicine

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Larvicidal, ovicidal, and repellent activities of the essential oil extracted by hydrodistillation from the leaves of the endemic Ethiopian plant Leucas stachydiformis (Hochst. ex Benth.) Briq were investigated against Anopheles arabiensis, the dominant malaria vector species in Ethiopia with the objective of searching for a plant-based malaria vector control strategy from medicinal plants. The larvicidal effect was tested against the fourth instar An. arabiensis wild larvae whilst freshly laid ova of An. arabiensis were used to determine the ovicidal activity of the essential oil at concentrations ranging from 6.25 to 400 ppm. Concentrations of 41.6–366.7 µg/cm2 were used to evaluate the repellent activity of the essential oil on 3–5 days old adult female An. arabiensis. The oil composition of L. stachydiformis was also analyzed using GC-MS. The study revealed that the oil possesses the highest larvicidal activity at 400 ppm and 200 ppm after 24 h and 48 h of treatment. LC50 values for the fourth larval instar after 24 h and 48 h of treatment were 43.4 ppm and 34.2 ppm, respectively. After 72 h of exposure, the oil displayed 100% ovicidal activity at 400 ppm with an IH50 value of 32.2 ppm. In the repellency test, at concentrations of 366.7, 133.3, and 41.6 µg/cm2, the oil gave a total percentage protection of 67.9 ± 4.2%, 37.2 ± 2.8%, and 32 ± 2.2%, respectively, for 4 h. The highest concentration (366.7 µg/cm2) gave 100% protection up to 90 min. GC-MS analysis of the oil revealed the presence of 24 compounds representing 90.34% of the total oil with caryophyllene oxide, germacrene D, and trans-caryophyllene constituting more than 50% of its components. Results of the present study suggest that the essential oil of L. stachydiformis has the potential to be used for the control of An. arabiensis mosquitoes.

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Larvicidal and repellent potential of Ageratum houstonianum against Culex pipiens

Cited by 5 publications

References 64 publications

Anti‐Culex pipiens activity of different pomegranate cultivars and determination of their bioactive compounds using LC‐MS profiling

Anti‐Culex pipiens activity of different pomegranate cultivars and determination of their bioactive compounds using LC‐MS profiling

Chemical composition of four essential oils and their adulticidal, repellence, and field oviposition deterrence activities against Culex pipiens L. (Diptera: Culicidae)

Larvicidal, Ovicidal, and Repellent Activities of Leucas stachydiformis (Hochst. ex Benth.) Briq Essential Oil against Anopheles arabiensis

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