Larvicidal Activities of Extract Flower Averrhoa bilimbi L. Towards Important Species Mosquito, Anopheles barbirostris (Diptera: Culicidae)

Sina, Ibnu; Zaharah,; Sabri, Mohd Shukri Mohd

doi:10.3923/ijzr.2016.25.31

Cited by 9 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarity in results was found by Vasanthi et al (2013) in case of Mugill cephalus due heavy metal pollution in Ennore Estuary. Coherence in results was found by Marchand et al (2012), Rajeshkumar et al (2015) and Sina et al (2015). Parallelism in results was found by Abdel-Moneim et al (2012) encase of Orechomius niloticus.…”

Section: Resultsmentioning

confidence: 67%

Histopathological alterations in gills, liver, kidney and muscles of Ictalurus punctatus collected from pollutes areas of River

Shahid

Sultana

et al. 2021

Braz. J. Biol.

View full text Add to dashboard Cite

Untreated sewage and industrial wastes from Faisalabad city are disposed to River Chenab through Chakbandi Main Drain (CMD). The present project is planned to investigate the effects of this freshwater pollution on the body of fish Ictalurus punctatus. The specimens of this fish species were collected upstream and downstream of the entrance of CMD into River Chenab. Fish gills, liver, kidney and muscles from dorsolateral regions of fish were subjected to histopathology. Farmed fish and fish from upstream areas were used as control. Fish collected from polluted experimental sites showed significant damage in selected organs. Gill tissues showed an abnormality in the form of an uplifting of the primary epithelium, fusion, vacuolation, hypertrophy, and necrosis. While liver tissues subjected to hepatocytes degeneration, necrosis, mitochondrial granular hepatocyte, and sinusoids dilation. Kidney tissues indicated increased bowmen space and constricted glomerulus and degenerated nephrons. Edema, necrosis, and atrophy were observed in muscle tissues of fish from polluted areas. Fish from the upstream area showed fused gill lamellae, inflammatory cell infiltration, hypertrophy and vacuolation in hepatocytes. Kidney tissues indicated the presence of nuclear tubular cells, destructive renal tubules, hemorrhage, and necrosis at tubular epithelium. Intra myofibril spaces were also observed in muscles. Specimens of control fish indicated no variation in gills, liver, kidney, and muscles. The present study revealed a strong correlation between the degree of tissue damage and environmental contamination. Present findings also compel global warnings to protect our water bodies and fish to rescue the human population.

show abstract

Section: Resultsmentioning

confidence: 67%

Histopathological alterations in gills, liver, kidney and muscles of Ictalurus punctatus collected from pollutes areas of River

Shahid

Sultana

et al. 2021

Braz. J. Biol.

View full text Add to dashboard Cite

show abstract

“…Secondary metabolites lower the surface tension of the mucosal membrane and as a result damage the digestive system. Damage in the midgut region changes various functions, such as osmoregulation, digestion, nutrition absorption, and ion transport ( Sina and Shukri, 2016 ). However, as in other insects, the mosquito’s stomach does not function only in digestion, but also in mechanical and chemical defense against invaders ( Terra, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Target and non-target effects of Foeniculum vulgare and Matricaria chamomilla combined extract on Culex pipiens mosquitoes

Galil

Abutaha

Al‐Doaiss

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

The present study focused on extracting green larvicides from extracts of the combination of Foeniculum vulgare and Matricaria chamomilla using different solvents of increasing polarity in a Soxhlet extractor and evaluating their ovicidal, larvicidal, and cytotoxic activities. The most promising among all tested extracts was hexane extract. The ovicidal activity of the hexane PH2 extract resulted in a significant (p < 0.05) decrease in egg hatchability from 95.00 ± 6.16% to 15 ± 9.04% at doses ranging from 62.5 to 500 µg/mL. The larval mortality with the hexane extract ranged from 13.33 ± 3.3% to 93.33 ± 3.3% at doses ranging from 31.25 to 250 µg/mL, respectively. The LC 50 and LC 90 values of the larvicidal activity of the hexane extract were estimated to be 148.3 and 242.17 µg/mL, respectively, after 24 h of exposure. Similarly, the LC 50 values after 48 and 72 h of exposure were 124.93 and 100.3 µg/mL, respectively, against the third instar of Cx. pipiens . PH2 treatment of larvae resulted in histopathological changes such as degenerated epithelial cells and destruction of microvilli on the epithelial cells. The PH2 extract achieved a dose-dependent decrease in the rate of cell survival. The IC 50 value of PH2-treated HUVECs was 192.07 µg/mL after 24 h of incubation. The cells showed changes in cellular and nuclear morphology. In conclusion, the hexane extract of PH2 could be used in mosquito management programs.

show abstract

“…aegypti larvae by lowering surface tension of the mucosa membrane in the digestive tract, making it easier to be damaged. Damage occurred in the midgut of larvae mainly because of various functions occuring in this place, such as digestion, nutrition absorption, ion transport, and osmoregulation [ 9 ]. The midgut is mainly composed of basal membrane-supported epithelium cells that layer body wall.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal Activity and Histopathological Effect of Averrhoa bilimbi Fruit Extract on Aedes aegypti from Surabaya, Indonesia

Rohmah

Subekti

Rudyanto

2020

Journal of Parasitology Research

View full text Add to dashboard Cite

Averrhoa bilimbi has been long thought to have biological activity. The aim of this study was to determine the activity of primary and secondary metabolites from A. bilimbi fruit extract on Aedes aegypti larvae mortality and midgut histopathology. Experiment was performed to third-instar Ae. aegypti larvae collected from Surabaya, which then exposed to A. bilimbi crude fruit extract at various concentration for 24 hours. After exposure, larvae were evaluated of its mortality and fixed in 2.5% neutral buffer formalin before processed and sectioned into histological slides and stained with hematoxylin-eosin (HE). Statistical analysis was performed using Spearman rank correlation to determine histopathological damage on midgut of Ae. aegypti larvae. Phytochemical screening of A. bilimbi crude fruit extract found that it contained saponins, tannins, and terpenoids. Minimum concentration able to induce mortality on Ae. aegypti larvae (LC50) was 977 ppm, while LC90 was at 1380 ppm. Severe alteration of larvae midgut was found after 24 hours exposure to 2000 ppm extract. Features of damage mostly found in larvae midgut were disruption of the microvilli, columnar cell vacuolization, epithelial nucleus crossed midgut lumen, and basal membrane damage. Damage caused by fruit extract in midgut of Ae. aegypti third instar larvae inhibited development of larvae. This study reported first finding of histopathological effect of A. bilimbi fruits extract on Ae. aegypti larvae midgut. Result of study was expected to contribute to better understand extract bioactivity of this plant to be applied as natural larvicide for Ae. aegypti.

show abstract

Larvicidal Activities of Extract Flower Averrhoa bilimbi L. Towards Important Species Mosquito, Anopheles barbirostris (Diptera: Culicidae)

Cited by 9 publications

References 5 publications

Histopathological alterations in gills, liver, kidney and muscles of Ictalurus punctatus collected from pollutes areas of River

Histopathological alterations in gills, liver, kidney and muscles of Ictalurus punctatus collected from pollutes areas of River

Target and non-target effects of Foeniculum vulgare and Matricaria chamomilla combined extract on Culex pipiens mosquitoes

Larvicidal Activity and Histopathological Effect of Averrhoa bilimbi Fruit Extract on Aedes aegypti from Surabaya, Indonesia

Contact Info

Product

Resources

About