Large-sized bone defect repair by combining a decalcified bone matrix framework and bone regeneration units based on photo-crosslinkable osteogenic microgels

When 2D nanomaterials meet biomolecules: design strategies and hybrid nanostructures for bone tissue engineering

“…The products aided the continuous proliferation of BMSCs for 14 days, with high cell viability and abundant ECM spread well on the surface. 87 For tissue engineering scaffolds, 2D nanosheets are combined with a variety of molecules, such as polypeptides (BFP), proteins (fibrin, bone morphogenetic protein), and polysaccharides (chitosan, cellulose). This process can improve the adhesion, proliferation, and differentiation of cells on the surface of the scaffold.…”

Acellular cartilage matrix biomimetic scaffold with immediate enrichment of autologous bone marrow mononuclear cells to repair articular cartilage defects

“…After 14 days of culture, the total RNA of each cell-scaffold construct (n ​= ​5 per group) was isolated using TRIzol reagent (Life Technologies, USA) and the RNA concentration was measured using a NanoDrop system (Thermo Scientific, USA) based on the manufacture's protocol. Reverse transcription was performed with a cDNA synthesis kit and gene expression was analyzed quantitatively with the SYBR Green using LightCycler® 480 Real-Time PCR system (Roche Ltd) according to a previously established method [ 25 ]. Forward and reverse primer sequences for collagen type Ⅱ alpha 1 (COL 2A1), aggrecan (ACAN), SRY-box transcription factor Ⅸ (SOX 9), and beta-actin housekeeping gene were designed based on published gene sequences from NCBI and PubMed ( Table S1 ).…”

Bone Engineering Scaffolds With Exosomes: A Promising Strategy for Bone Defects Repair

“…Therefore, processed and modified allogeneic bones overcome their own shortcomings and become the most available grafts, considered the best alternative to autografts. Demineralized bone matrix (DBM) is a highly processed allogeneic graft, which is often used to fill in bone defects ( Hao et al, 2022 ). Similarly, heterogenous bone grafts face the same concerns, such as immunogenicity and disease transference ( Amiryaghoubi et al, 2020 ).…”