Acellular cartilage matrix biomimetic scaffold with immediate enrichment of autologous bone marrow mononuclear cells to repair articular cartilage defects

Jia, Litao; Zhang, Peiling; Ci, Zheng; Hao, Xiaoyan; Bai, Baoshuai; Zhang, Wei; Jiang, Haiyue; Zhou, Guangdong

doi:10.1016/j.mtbio.2022.100310

Cited by 13 publications

(11 citation statements)

References 57 publications

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“…Articular cartilage defect is usually accompanied by subchondral bone degeneration, which is an important and intractable clinical problem [ [1] , [2] , [3] , [4] , [5] , [6] , [7] ]. At present, tissue engineering is emerging as a promising alternative approach: seeding cells on biomaterials to reconstruct desired substitutes for tissue repair [ [8] , [9] , [10] , [11] , [12] ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Hua

Huo

Bai³

et al. 2022

Materials Today Bio

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Section: Introductionmentioning

confidence: 99%

Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Hua

Huo

Bai³

et al. 2022

Materials Today Bio

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“…[ 13,14,19 ] Therefore, in this study, we fabricated novel WJ‐based hydrogels for cartilage regeneration. Compared with complex techniques, such as those used for making decellularized cartilage ECM of cartilage sheets, [ 20 ] cartilage powder, [ 18b,21 ] and laser micropores, [ 22 ] a simple and mild decellularization process effectively removed the cellular components of WJ while retaining the content of collagen, HA and GAGs and the structure of WJ in this study. The resulting DWJMA hydrogels demonstrated rapid gelation under UV light and had tunable swelling capability, degradability, and mechanical properties, indicating that they can be used for regenerating various tissues.…”

Section: Discussionmentioning

confidence: 99%

C‐Shaped Cartilage Development Using Wharton's Jelly‐Derived Hydrogels to Assemble a Highly Biomimetic Neotrachea for use in Circumferential Tracheal Reconstruction

Gao

Wang

et al. 2023

Adv Funct Materials

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A highly biomimetic neotrachea with C-shaped cartilage rings has promising clinical applications in the treatment of circumferential tracheal defects (CTDs) owing to its structure and physiological function. However, to date, most fabricated tracheal cartilages are O-shaped. In this study, finite element analysis demonstrates C-shaped cartilage rings that exhibit better compliance than O-shaped. Hydrogel is developed using methacryloyl-modified decellularized Wharton's jelly matrix (DWJMA) for the regeneration of C-shaped cartilage rings. This novel hydrogel possesses adjustable physicochemical properties and favorable cytocompatibility. When loaded with chondrocytes, DWJMA hydrogels support the optimal cartilage regeneration both in vitro and in vivo. More importantly, a highly biomimetic neotrachea simultaneously simulating the structural and physiological properties of the normal trachea is regenerated via modular assembly of several individual C-shaped cartilage rings. The results demonstrate the highly biomimetic neotrachea have better patency (88.6 ± 6.1% vs 74.4 ± 9.4%, p < 0.05), improve the survival rate, alleviate weight loss and mucoid impaction, than its O-shaped counterpart when used for the treatment of CTDs in a rabbit model. Therefore, this study proposes a novel hydrogel for the regeneration of C-shaped cartilage and provides new insights into the treatment of CTDs using a highly biomimetic neotrachea with C-shaped cartilage rings.

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“…This might be due to the presence of different growth factors and extracellular matrix in CdECM, as indicated by previous reports. 53 Following the incubation, the viability of the BMSCs in different hydrogels was generally comparable. However, a notable difference was observed on day 7, with a higher cell density observed in CdECM in comparison to HAMA and the hybrid hydrogel.…”

Section: Cell Viability and Chondrogenesis-related Gene Expression Ev...mentioning

confidence: 93%

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

Li,

Jiang,

Zhang

et al. 2024

ACS Appl. Polym. Mater.

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Articular cartilage defects caused by trauma or osteoarthritis remain a significant challenge in clinical practice due to their poor self-healing capacity. Current clinical approaches are generally limited by their complex procedures or certain postoperation complications. Therefore, it is crucial to develop an effective strategy for one-step cartilage repair with long-term functional cartilage regeneration. This study reports the creation of an injectable hydrogel composed of cartilage decellularized extracellular matrix (CdECM) and hyaluronic acid methacrylate (HAMA). In the presence of photoinitiator ruthenium (Ru)/sodium persulfate (SPS), visible light (450 nm) not only triggered the polymerization of CdECM and HAMA to form stable hydrogel in 1 min but also facilitated the tyrosine cross-links between biotissue and CdECM without any further moiety grafting, which cannot be achieved by routinely used photoinitiators and provides the hybrid hydrogel with bioadhesive properties. This dual-network hybrid hydrogel demonstrated robust adhesion strength to cartilage and significantly enhanced mechanical performance in comparison to that of CdECM or HAMA alone. Further in vitro culture demonstrated the promotive effect of hybrid hydrogel on encapsulated porcine bone marrow mesenchymal stem cells toward chondrogenic differentiation after 21 days of incubation compared with HAMA. Through in vivo subcutaneous implantation in a mouse model for two and 4 weeks, we further illustrated that the hybrid hydrogel has improved biocompatibility in comparison to HAMA alone. Thus, the presented hydrogel is a promising biomaterial for cartilage regeneration through minimally invasive arthroscopic injection in clinical practice.

show abstract

Acellular cartilage matrix biomimetic scaffold with immediate enrichment of autologous bone marrow mononuclear cells to repair articular cartilage defects

Cited by 13 publications

References 57 publications

Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

Fabrication of biphasic cartilage-bone integrated scaffolds based on tissue-specific photo-crosslinkable acellular matrix hydrogels

C‐Shaped Cartilage Development Using Wharton's Jelly‐Derived Hydrogels to Assemble a Highly Biomimetic Neotrachea for use in Circumferential Tracheal Reconstruction

Cartilage Decellularized Extracellular Matrix-Based Hydrogel with Enhanced Tissue Adhesion and Promoted Chondrogenesis for Cartilage Tissue Engineering

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