Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice

Li, Gezi; Wu, Yuh Lin; Liu, Guoyu; Xiao, Xiang-Hong; Wang, Pengfei; Gao, Tian; Xu, Mei; Han, Qinghua; Wang, Yonghua; Guo, Tao; Kang, Guozhang

doi:10.1074/mcp.ra117.000032

Cited by 51 publications

(45 citation statements)

References 80 publications

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“…As previously reported by Li et al (2017) and Troufflard et al (2010), we also observed induction in JA content due to K deficiency though the increase is meager which may be due to the prolonged period of the deficiency, i.e. 15 days or the milder K deficiency conditions as compared to the conditions used in previous reports.…”

Section: Resultssupporting

confidence: 86%

“…By constitutively overexpressing a JAZ repressor we may have disturbed that finely tuned JA homeostasis resulting in enhanced JA levels and signaling in OsJAZ9 overexpression plants as also reported earlier in Arabidopsis (Thatcher et al, 2016). Noticeably, JA levels were also enhanced in knockdown lines of AJAZ1 while remaining unchanged in the knockdown lines of NaZAZh (Li et al, 2017; Oh et al, 2012). Further research is needed to pinpoint the chief regulator of JA negative feedback regulation on JAZ overexpression.…”

Section: Discussionmentioning

confidence: 96%

“…Root growth patterns during K deficiency mimic the root phenotype observed on JA treatment in various plants (Staswick et al, 1992; Gruber et al, 2013; Cai et al, 2014). Recently, Troufflard et al (2010) and Li et al (2017) have found enhanced JA levels during K deficiency in Arabidopsis, rice, and wheat. JAZ repressors are pivotal to JA signaling, and their manipulation leads to many altered plant processes (Wasternack and Song, 2017).…”

Section: Discussionmentioning

confidence: 99%

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OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

Pandey

Mehra

et al. 2018

Preprint

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Potassium (K) which makes around 2-10% of plants total dry biomass, when become deficient, makes the plants highly susceptible to both abiotic and biotic stresses. Recent evidences suggest overlapping transcriptional responses to K deficiency and Jasmonate (JA) treatment in plants. However, a link between these responses was missing. Notably, K deficiency and JA application produce similar phenotypic and transcriptional responses. Here, we used molecular, physiological and morphological studies to analyze the role of OsJAZ9 in JA homeostasis, K deficiency and sheath blight resistance. We raised OsJAZ9 overexpression, knockdown, translational reporter and C-terminal deleted translational reporter lines in rice to establish the role of JA signaling in K ion homeostasis and OsJAZ9 as a critical component of JA signaling for K deficiency response. OsJAZ9 overexpression and knockdown provide K deficiency tolerance and sensitivity, respectively, by modulating various K transporters and root system architecture. Furthermore, RNA Seq and JA profiling revealed an elevation of JA responsive genes and JA levels in OsJAZ9 OE lines under K deficiency. Our data provide clear evidence on the crucial role of JAZ repressor, OsJAZ9 in improving K deficiency tolerance in rice by altering JA levels and signaling.

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Section: Resultssupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

Pandey

Mehra

et al. 2018

Preprint

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“…In plants, photosynthesis is an important metabolic process and is susceptible to environmental stress. It has been reported that photosynthesis rate was promoted in Arabidopsis under drought stress [69]. In this study, iTRAQ data show that MeJA application could remarkably enhance the expression of photosynthesis-related proteins.…”

Section: Photosynthesis-related Proteinsmentioning

confidence: 49%

iTRAQ-Based Quantitative Proteomic Analysis of the Arabidopsis Mutant opr3-1 in Response to Exogenous MeJA

Zhao

2020

IJMS

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Jasmonates (JAs) regulate the defense of biotic and abiotic stresses, growth, development, and many other important biological processes in plants. The comprehensive proteomic profiling of plants under JAs treatment provides insights into the regulation mechanism of JAs. Isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis was performed on the Arabidopsis wild type (Ws) and JA synthesis deficiency mutant opr3-1. The effects of exogenous MeJA treatment on the proteome of opr3-1, which lacks endogenous JAs, were investigated. A total of 3683 proteins were identified and 126 proteins were differentially regulated between different genotypes and treatment groups. The functional classification of these differentially regulated proteins showed that they were involved in metabolic processes, responses to abiotic stress or biotic stress, the defense against pathogens and wounds, photosynthesis, protein synthesis, and developmental processes. Exogenous MeJA treatment induced the up-regulation of a large number of defense-related proteins and photosynthesis-related proteins, it also induced the down-regulation of many ribosomal proteins in opr3-1. These results were further verified by a quantitative real-time PCR (qRT-PCR) analysis of 15 selected genes. Our research provides the basis for further understanding the molecular mechanism of JAs’ regulation of plant defense, photosynthesis, protein synthesis, and development.

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“…ITRAQ proteome assessment showed that the differentially expressed proteins in wheat under low potassium stress showed organ-specific differences. In addition, most of the differently expressed proteins associated with hormone synthesis were found to be involved in JA synthesis [28].Banana (Musa acuminata L.) is a kind of large herbaceous tropical and subtropical fruit tree. It is considered to be a potassium-loving fruit tree.…”

mentioning

confidence: 99%

Transcriptome Changes Induced by Different Potassium Levels in Banana Roots

Lin

et al. 2019

Plants

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Potassium plays an important role in enhancing plant resistance to biological and abiotic stresses and improving fruit quality. To study the effect of potassium nutrient levels on banana root growth and its regulation mechanism, four potassium concentrations were designed to treat banana roots from no potassium to high potassium. The results indicated that K2 (3 mmol/L K 2 SO 4 ) treatment was a relatively normal potassium concentration for the growth of banana root, and too high or too low potassium concentration was not conducive to the growth of banana root. By comparing the transcriptome data in each treatment in pairs, 4454 differentially expressed genes were obtained. There were obvious differences in gene function enrichment in root systems treated with different concentrations of potassium. Six significant expression profiles (profile 0, 1, 2, 7, 9 and 13) were identified by STEM analysis. The hub genes were FKF1, HsP70-1, NRT1/PTR5, CRY1, and ZIP11 in the profile 0; CYP51 in profile 1; SOS1 in profile 7; THA, LKR/SDH, MCC, C4H, CHI, F3 H, 2 PR1s, BSP, TLP, ICS, RO, chitinase and peroxidase in profile 9. Our results provide a comprehensive and systematic analysis of the gene regulation network in banana roots under different potassium stress.Plants 2020, 9, 11 2 of 24 area [18]. The lack of potassium had a significant effect on the growth of rapeseed, and the lack of potassium significantly inhibited the growth of taproots and lateral roots [19]. Potassium deficiency of tobacco decreased root growth and mainly affected the formation and elongation of primary lateral roots [20]. Low potassium not only significantly reduced the dry weight of cabbage root, but also reduced its leaf area [21]. Therefore, the morphological structure of root system was greatly affected by potassium stress. It is significant to study the response mechanism of root system under potassium stress. There has been extensive research on root structural changes in response to stress of nutrients such as phosphate and nitrate and the signaling pathways that mediate these changes [22], and practical breakthroughs have been made. However, the mechanism of root structural change and response to potassium stress is still unclear.With the purpose to explore the molecular mechanism of plant root response to potassium pressure, there are more and more reports on transcriptome, metabolome and proteomics of potassium stress (especially low potassium stress) in recent years. Transcriptome responses to K starvation in Arabidopsis thaliana [16], rice [4], soybean [23] and wild barley [24] indicated that genes related to ion transport, metabolism, signal transduction and protein phosphorylation significantly changed. Transcriptional analysis of sugarcane under low potassium stress showed significant differences in the expression of transcription factors, ion transporters, protein kinases and genes related to oxidative stress in the Ca 2+ signal and ethylene signal pathways [25]. The results of transcriptome of rice potassium deficient seedlings show...

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Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice

Cited by 51 publications

References 80 publications

OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

iTRAQ-Based Quantitative Proteomic Analysis of the Arabidopsis Mutant opr3-1 in Response to Exogenous MeJA

Transcriptome Changes Induced by Different Potassium Levels in Banana Roots

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