Large Scale Production of Liquid Wax Ester by Immobilized Lipase

Abstract

Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 , amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, 90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage ( 80%) up to 4 cycles.

Response surface methodological approach for optimizing production of geranyl propionate catalysed by carbon nanotubes nanobioconjugates

“…[29] As the reaction proceeded, the substrate concentration decreased, which consequently reduced the degree of saturation of the enzyme with the substrate. [30] It can be seen that the percentage yield increased slightly with the increasing of the solvent hydrophobicity (log P) up to 6 h. The observed increase in yield was attributed to the activity of the enzyme, which was affected by the polarity of the employed organic solvent. [31] Corroborating previous reports has shown that solvent with a higher log P had a better rate of esterification.…”

Formulation and in vitro Evaluation of Ketoprofen in Palm Oil Esters Nanoemulsion for Topical Delivery

“…Among of the works are syntheses of esters from palm oil. Alcoholysis of triglycerides from palm oil to produce palm oil esters using lipase as a catalyst is a relatively simple process and moreover the starting materials are cheap [3][4][5][6] . Synthesis or modification of lipids for example; modification of Palm Oil into Palm Oil Esters (POEs) has grown due to the possibility of obtaining a wide variety of high quality natural products under mild reaction conditions utilizing selective enzyme substrate as environment friendly biocatalysts 2,7) .…”

Kinetics of Enzymatic Synthesis of Liquid Wax Ester from Oleic Acid and Oleyl Alcohol

“…High performance enzymatic synthesis of a liquid wax ester using immobilized lipase from C. antartica has been previously reported by Radzi et al 9) and the optimized reaction conditions was achieved through response surface methodology 10) . The high conversion and productivity of wax ester was further studied in large scale production using batch mode stirred tank reactor with one-multi bladed impeller 11) .…”