Large-Scale Production of Lambda Bacteriophage and Purified Lambda Deoxyribonucleic Acid

Abstract

Large amounts of a heat-inducible phage lambda mutant (λCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken λ DNA molecules.