Characteristics of PRD1, a Plasmid-Dependent Broad Host Range DNA Bacteriophage

Olsen, Ronald H.; Siak, J.; Gray, Robert H.

doi:10.1128/jvi.14.3.689-699.1974

Cited by 268 publications

(114 citation statements)

References 31 publications

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“…The six other viruses studied are previously characterized isolates from different environments. PRD1 is a non-tailed icosahedral double-stranded DNA (dsDNA) phage with an internal membrane Cockburn et al, 2004), originating from sewage (Olsen et al, 1974). Phage P22 is a short-tailed dsDNA phage (Lander et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Virus–host interactions in environments with a wide range of ionic strengths

Kukkaro

Bamford²

2009

Environ Microbiol Rep

107

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Although high-salt environments are rich in viruses, virus-host interactions have not been much explored. Here we study the virus-host interactions occurring in a variety of salt environments, up to saturated salinity. We chose viruses from different environments with diverse morphologies that included both bacterial and archaeal viruses. To extend the test virus set five new haloviruses were isolated and initially characterized in this study. We observed adsorption rates that varied over four orders of magnitude among the virus-host cell systems used in this study. Changes in ionic strength affected the adsorption of these viruses to their host cells in a variety of ways. All the studied viruses, regardless from which environment they were isolated, were more resistant to variations in ionic strength conditions than their host cells. Our study provides a glimpse of the early events in virus life cycles for a number of viruses from different environments. We also gained information on viral responses to changing environments, a valuable piece of information in extending our understanding of the viruses in the environment.

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Section: Introductionmentioning

confidence: 99%

Virus–host interactions in environments with a wide range of ionic strengths

Kukkaro

Bamford²

2009

Environ Microbiol Rep

107

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“…Bacteriophages are useful model systems because they and their hosts are amenable to genetic and biochemical studies (Mindich and Bamford, 1988;Bamford et al, 1995). PRD1 (Olsen et al, 1974) is the type member of a commonly occurring group, the Tectiviridae (Francki et al, 1991). PRD 1 and PR4 are the best characterized of this group and are closely related (Bamford et al, 1981;Myung et al, 1994;Savilahti and Bamford, 1986) PRD1 infects Gram-negative bacteria which contain plasmids belonging to the P, W or N incompatability groups (Bradley, 1974;Olsen et al, 1974;Bradley and Rutherford, 1975).…”

Section: Introductionmentioning

confidence: 99%

“…PRD1 (Olsen et al, 1974) is the type member of a commonly occurring group, the Tectiviridae (Francki et al, 1991). PRD 1 and PR4 are the best characterized of this group and are closely related (Bamford et al, 1981;Myung et al, 1994;Savilahti and Bamford, 1986) PRD1 infects Gram-negative bacteria which contain plasmids belonging to the P, W or N incompatability groups (Bradley, 1974;Olsen et al, 1974;Bradley and Rutherford, 1975). These plasmids encode the receptor complex for the bacteriophage (Davis et al, 1982;Kotilainen et al, 1993;Pansegrau et al, 1994), which also functions as a DNA transfer complex in conjugation (Dreiseikelmann, 1994;Lessl and Lanka, 1994).…”

Section: Introductionmentioning

confidence: 99%

DNA packaging orders the membrane of bacteriophage PRD1.

1995

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Bacteriophage PRD1 contains a linear dsDNA genome enclosed by a lipid membrane lying within a protein coat. Determination of the structure of the detergenttreated particle to 2 nm by cryo-electron microscopy and three-dimensional reconstruction has defined the position of the major coat protein P3. The coat contains 240 copies of trimeric P3 packed into positions of local 6-fold symmetry on a T = 25 lattice. The threedimensional structures of the PRD1 virion and a DNA packaging mutant to a resolution of 2.8 nm have revealed specific interactions between the coat and the underlying membrane. The membrane is clearly visible as two leaflets separated by 2 nm and spanned by transmembrane density. The size of the coat does not change upon DNA packaging. Instead, the number of interactions seen between the protein shell and the membrane and the order of the membrane components increase. Thus the membrane of PRD1 plays a role in assembly which is akin to that played by the nucleocapsid in other membrane viruses.

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“…Bacteriophage PRD1 is the prototype of the Tectiviridae family, with virions characterized by their unusual possession of an internal membrane that lies beneath the icosahedral protein capsid. PRD1 infects a great variety of Gram-negative hosts, including Escherichia, Pseudomonas, Salmonella, Proteus, Vibrio and Acinetobacter species (Olsen et al, 1974). Like in the case of Φ29TP, the priming residue (Tyr 190) is located in the C-terminal region of the PRD1 terminal protein (PRD1TP) (Shiue et al, 1991), but functional domains for DNA polymerase interaction or DNA binding have not been identified.…”

Section: Prd1 Tp Truncated Mutants With Impaired Nucleoid Targeting Mmentioning

confidence: 99%

Nuclear and nucleoid localization are independently conserved functions in bacteriophage terminal proteins

Redrejo-Rodríguez

Muñoz‐Espín

Holguera

et al. 2013

Molecular Microbiology

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SummaryBacteriophage terminal proteins (TPs) prime DNA replication and become covalently linked to the DNA 5′-ends. In addition, they are DNA-binding proteins that direct early organization of phage DNA replication at the bacterial nucleoid and, unexpectedly, contain nuclear localization signals (NLSs), which localize them to the nucleus when expressed in mammalian cells. In spite of the lack of sequence homology among the phage TPs, these three properties share some common features, suggesting a possible evolutionary common origin of TPs. We show here that NLSs of three different phage TPs, Φ29, PRD1 and Cp-1, are mapped within the protein region required for nucleoid targeting in bacteria, in agreement with a previously proposed common origin of DNA-binding domains and NLSs. Furthermore, previously reported point mutants of Φ29 TP with no nuclear localization still can target the bacterial nucleoid, and Cp-1 TP contains two independent NLSs, only one of them required for nucleoid localization. Altogether, our results show that nucleoid and nucleus localization sequence requirements partially overlap, but they can be uncoupled, suggesting that conservation of both features could have a common origin but, at the same time, they have been independently conserved during evolution.

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Characteristics of PRD1, a Plasmid-Dependent Broad Host Range DNA Bacteriophage

Cited by 268 publications

References 31 publications

Virus–host interactions in environments with a wide range of ionic strengths

Virus–host interactions in environments with a wide range of ionic strengths

DNA packaging orders the membrane of bacteriophage PRD1.

Nuclear and nucleoid localization are independently conserved functions in bacteriophage terminal proteins

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