Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine

Malinauskas, Mangirdas; Purlys, Vytautas; Žukauskas, Andrius; Rutkauskas, Marius; Danilevičius, Paulius; Paipulas, Domas; Bickauskaite, Gabija; Bukelskis, Laurynas; Baltriukienė, Daiva; Širmenis, Raimondas; Gaidukevičiūtė, Arūnė; Bukelskienė, Virginija; Gadonas, R.; Sirvydis, Vytautas; Piskarskas, A.; Vainos, Nikolaos; Couris, Stelios; Paspalakis, Emmanuel; Koutselas, Ioannis; Pissadakis, Stavros

doi:10.1063/1.3521344

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…The potential of composite scaffold approach could be applicable for single or multiple cell adhesion, migration, proliferation and differentiation mechanism study in 3D as well as suggesting the answer for best material chemistry and architecture combinations for scaffold needed in tissue engineering and regenerative medicine. [35][36][37]…”

Section: D Polymerization: Structuring Without Photo-initiatorsmentioning

confidence: 99%

Three-dimensional nanostructuring of polymer materials by controlled avalanche using femtosecond laser pulses

et al. 2014

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We report direct laser fabrication of free-standing 3D structures in a sol-gel photo-polymer SZ2080, poly(ethylene glycol) diacrylate (PEG-DA-700) and thermo-polymer polydimethylsiloxane (PDMS) without use of two-photon absorbing photo-sensitizers. By estimating the multi-photon and avalanche ionization rates in the focal volume it is shown that bulk structuring of pure materials was achieved via a controlled avalanche. It is shown that several non-photosesitized materials can be combined for fabrication of composite material structures evoking a possibility to create non-toxic biocompatible scaffolds for tissue engineering, transparent microoptical elements and higher damage threshold photonic devices.

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Section: D Polymerization: Structuring Without Photo-initiatorsmentioning

confidence: 99%

Three-dimensional nanostructuring of polymer materials by controlled avalanche using femtosecond laser pulses

et al. 2014

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“…It combines the true 3D capability, 9 nanoscale resolution 10 and high efficiency (fabrication throughput) required for industrial applications. 11,12 Furthermore, it enables beam shaping techniques 13 to be implemented for a parallel processing via holography 14 or even direct image printing. 15 The quest for higher resolution is actively debated and is currently an object of intense study.…”

Section: Introductionmentioning

confidence: 99%

Mesoscale 3D manufacturing: varying focusing conditions for efficient direct laser writing of polymers

Jonušauskas

Malinauskas

2014

Nanophotonics V

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In this paper, we report a novel approach for efficient fabrication of mesoscale polymer 3D microstructures. It is implemented by direct laser writing varying exposure beam focusing conditions. By carefully optimizing the fabrication parameters (laser intensity, scanning velocity/exposure time, changing objective lens) complex 3D geometries of the microstructures can be obtained rapidly. Additionally, we demonstrate this without the use of the photoinitiator as photosensitizer doped in the pre-polymer material (SZ2080). At femtosecond pulsed irradiation ∼TW/cm 2 intensities the localized free radical polymerization is achieved via avalanche induced bond braking. Such microstructures have unique biocompatibility and optical transparency as well as optical damage threshold value. By creating the bulk part of the structure using low-NA (0.45) objective and subsequently fabricating the fine features using oil immersion high-NA (1.4) objective the manufacturing time is reduced dramatically (30 x is demonstrated). Using this two objective method a prototype of functional microdevice was produced: 80 and 85 µm diameter microfluidic tubes with the fine filter consisting of 4 µm period grating structure that has 400 nm wide threads, which corresponds to a feature precision aspect ratio of ∼200. Therefore, such method has great potential as a polymer fabrication tool for mesoscale optical, photonic and biomedical applications as well as highly integrated 3D µ-systems. Furthermore, the proposed approach is not limited to lithography and can be implemented in a more general type of laser writing, such as inscription within transparent materials or substractive manufacturing by ablation.

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“…36 Due to its exclusive advantages, multi-photon polymerization has already established its applications in fabrication of photonics, 37 microoptics, 38 microfluidics 39 as well as biomedical devices. 23,40 In this work we present the latest results of DLW application in TE by fabricating 3D artificial scaffolds for stem cell growing out of hybrid organic-inorganic biomaterials. Critical parameters for high throughput fabrication were defined and high quality structures were fabricated.…”

Section: Introductionmentioning

confidence: 99%

Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering

Danilevičius

2012

J. Biomed. Opt

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This work presents the latest results on direct laser writing of polymeric materials for tissue engineering applications. A femtosecond Yb:KGW laser (300 fs, 200 kHz, 515 nm) was used as a light source for non-linear lithography. Fabrication was implemented in various photosensitive polymeric materials, such as: hybrid organic-inorganic sol-gel based on silicon-zirconium oxides, commercial ORMOCER® class photoresins. These materials were structured via multi-photon polymerization technique with submicron resolution. Porous three-dimensional scaffolds for artificial tissue engineering were fabricated with constructed system and were up to several millimeters in overall size with 10 to 100 μm internal pores. Biocompatibility of the used materials was tested in primary rabbit muscle-derived stem cell culture in vitro and using laboratory rats in vivo. This interdisciplinary study suggests that proposed technique and materials are suitable for tissue engineering applications.

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Large Scale Laser Two-Photon Polymerization Structuring for Fabrication of Artificial Polymeric Scaffolds for Regenerative Medicine

Cited by 10 publications

References 18 publications

Three-dimensional nanostructuring of polymer materials by controlled avalanche using femtosecond laser pulses

Three-dimensional nanostructuring of polymer materials by controlled avalanche using femtosecond laser pulses

Mesoscale 3D manufacturing: varying focusing conditions for efficient direct laser writing of polymers

Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering

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